中国-哈萨克斯坦边境阿拉山口口岸地区凶小库蚊中首次检测到沃尔巴克氏体
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摘要
目的调查中国-哈萨克斯坦(中哈)边境阿拉山口口岸地区优势蚊种凶小库蚊携带沃尔巴克氏体情况及其基因型,为防控蚊媒传播疾病提供依据。方法 2015年4-10月,在中哈边境一区、二区、阿拉山口艾比湖湿地自然保护区,利用诱蚊灯和挥网法采集蚊虫,PCR扩增沃尔巴克氏体wsp基因,测序后进行Blast分析,利用Mega 6.0软件构建分子遗传进化树。结果凶小库蚊感染沃尔巴克氏体的阳性率为3.33%(2/60);wsp基因遗传进化树显示,沃尔巴克氏体wsp基因序列属于A(w Alb A)和B(w Alb B)2个超级基因组。结论中哈边境阿拉山口口岸地区首次检测到凶小库蚊携带沃尔巴克氏体,其基因型为w Alb A和w Alb B型,为应用沃尔巴克氏体通过种群替换阻断蚊媒疾病的传播等防控技术提供了基础资料。
Objective To investigate the infection and genotype of Wolbachia from Culex modestus at Alataw port, China-Kazakhstan border.Method sSweeping Netting and Light Trapping were utilized to collect adult mosquitoes at the Yiqu,Erqu and Aibi Lake, wsp genes of Wolbachia were detected by polymerase chain reaction(PCR), the sequence analysis byBlast and phylogenetic analysis by Mega 6.0.Results The positive rate of wsp gene of Wolbachia was 3.33%(2/60) atAlataw port. The wsp haplotype sequences were divided in Supergroup A(w Alb A) and B(w Alb B) by phylogenetic analysis.Conclusion The w Alb A and w Alb B Wolbachia exists in Cx. modestusat Alataw China-Kazakhstan port.
Objective To investigate the infection and genotype of Wolbachia from Culex modestus at Alataw port, China-Kazakhstan border.Method sSweeping Netting and Light Trapping were utilized to collect adult mosquitoes at the Yiqu,Erqu and Aibi Lake, wsp genes of Wolbachia were detected by polymerase chain reaction(PCR), the sequence analysis byBlast and phylogenetic analysis by Mega 6.0.Results The positive rate of wsp gene of Wolbachia was 3.33%(2/60) atAlataw port. The wsp haplotype sequences were divided in Supergroup A(w Alb A) and B(w Alb B) by phylogenetic analysis.Conclusion The w Alb A and w Alb B Wolbachia exists in Cx. modestusat Alataw China-Kazakhstan port.
引文
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[15]徐晓晗,奚志勇,郑小英.沃尔巴克氏体诱导细胞质不相容的模型和分子机制研究进展[J].国际医学寄生虫病杂志,2015,42(4):244-248.
[16]Walker T,Johnson PH,Moreira LA,et al.The w Mel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations[J].Nature,2011,476(7361):450-453.
[17]Hughes H,Britton NF.Modelling the use of Wolbachia to controldengue fever transmission[J].Bull Math Biol,2013,75(5):796-818.
[18]Turley AP,Moreira LA,ONeill SL,et al.Wolbachia infectionreduces blood-feeding success in the dengue fever mosquito,Aedes aegypti[J].PLo S Negl Trop Dis,2003,3(9):e516.
[19]申卉,徐静.明年试投“益蚊”控制登革热[EB/OL].(2014-11-07)[2016-11-07].http://news2.sysu.edu.cn/news03/140938.htm.
[2]林立丰,吴德,张欢,等.广东省蚊虫感染沃尔巴克氏体初步调查研究[J].中国媒介生物学及控制杂志,2014,25(2):113-115,118.
[3]潘晓玲,刘起勇,奚志勇,等.基于昆虫共生菌沃尔巴克氏体的蚊媒和蚊媒病控制研究进展[J].中国媒介生物学及控制杂志,2014,25(1):1-7.
[4]尹小平,郑重,田延河,等.新疆中哈边境阿拉山口口岸蚊虫调查研究[J].寄生虫与医学昆虫学报,2014,21(3):180-183.
[5]Hertig M,Wolbach SB.Studies on rickettsia-like micro-organismsin insects[J].J Med Res,1924,44(3):329-374,377.
[6]Castelli F,Cabona MG,Brunori A,et al.Short report:importedmosquito:an uninvited guest[J].Am J Trop Med Hyg,1994,50(5):548-549.
[7]Berry RL,Peterson ED,Restifo RA.Records of imported tire-breeding mosquitoes in Ohio[J].J Am Mosq Control Assoc,1988,4(2):187-189.
[8]Ramsdale CD.Anopheles mosquitoes and imported malaria inLibya[J].Mosq Syst,1990,22(1):34-40.
[9]徐卫民,项海青.西尼罗热研究进展[J].浙江预防医学,2005,17(1):54-55.
[10]刘然,张桂林,孙响,等.新疆艾比湖布尼亚病毒的分离与分子生物学鉴定[J].中华流行病学杂志,2014,35(8):939-942.
[11]赵睿.上海3个海港口岸入境船舶蚊媒传播防制现状及对策研究[D].上海:复旦大学,2012.
[12]史书路,于长友,王雪嵋,等.蚊媒传染病传入我国风险及防控策略[J].口岸卫生控制,2015,20(1):5-6.
[13]Aliota MT,Peinado SA,Velez ID,et al.The w Mel strain ofWolbachia reduces transmission of Zika virus by Aedes aegypti[J].Sci Rep,2016,6:28792.
[14]Dutra HLC,Rocha MN,Dias FBS,et al.Wolbachia blockscurrently circulating Zika virus isolates in Brazilian Aedes aegypti mosquitoes[J].Cell Host Microbe,2016,19(6):771-774.
[15]徐晓晗,奚志勇,郑小英.沃尔巴克氏体诱导细胞质不相容的模型和分子机制研究进展[J].国际医学寄生虫病杂志,2015,42(4):244-248.
[16]Walker T,Johnson PH,Moreira LA,et al.The w Mel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations[J].Nature,2011,476(7361):450-453.
[17]Hughes H,Britton NF.Modelling the use of Wolbachia to controldengue fever transmission[J].Bull Math Biol,2013,75(5):796-818.
[18]Turley AP,Moreira LA,ONeill SL,et al.Wolbachia infectionreduces blood-feeding success in the dengue fever mosquito,Aedes aegypti[J].PLo S Negl Trop Dis,2003,3(9):e516.
[19]申卉,徐静.明年试投“益蚊”控制登革热[EB/OL].(2014-11-07)[2016-11-07].http://news2.sysu.edu.cn/news03/140938.htm.