高效液相色谱法同时测定蒙药森登-4中绿原酸和槲皮素含量
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摘要
建立反相高效液相色谱法(RP-HPLC)同时测定蒙药森登-4中绿原酸和槲皮素含量的方法.采用超声提取方法作为样品提取方法制备供试品溶液.分析色谱柱为romasil C18(250 mm×4.6 mm,5μm)色谱柱;流动相为甲醇∶水(加0.02%的磷酸)=45∶55;检测波长为360 nm;流速为0.8 mL·min-1,柱温为室温.结果显示,绿原酸和槲皮素分别在17.6μg·mL~(-1)～132μg·mL~(-1)和2.16μg·mL~(-1)～16.2μg·mL~(-1)浓度范围内具有良好的线性关系,其相关系数分别为0.9994和0.9996.平均加样回收率测定结果分别为97.3%和98.2%(n=6),相应的RSD值分别为1.2%和1.6%.该方法简便、快速,方法重现性、稳定性和可靠性良好,可作为蒙药森登-4中绿原酸和槲皮素含量测定方法.
A high-performance liquid chromatography method(HPLC)for determination of chlorogenic acid and quercetin in Mongolian medicine Sendeng-4 was established. Preparation of sample solution was carried out with ultrasonic extraction. The chromatographic column was romasil C18(250 mm×4.6 mm,5 μm),the mobile phase was methanol:water(0.02% phosphoric acid added)=45∶55,the detection wavelength was 360 nm,the flow rate was0.8 m L·min-1,and the column temperature was room temperature. There was a good linear relationship between chlorogenic acid and quercetin in the range of 17.6 μg·mL~(-1)～132 μg·mL~(-1) and 2.16 μg·mL~(-1)～16.2 μg·mL~(-1),with correlation coefficients of 0.9994 and 0.9996,respectively. The average recovery was 97.3% and 98.2%,respectively(n=6),and the corresponding RSD was 1.2% and 1.6%,respectively. The method is simple and rapid,with good reproducibility and stability,and can be used for the determination of chlorogenic acid and quercetin in Mongolian medicine sendeng-4.
A high-performance liquid chromatography method(HPLC)for determination of chlorogenic acid and quercetin in Mongolian medicine Sendeng-4 was established. Preparation of sample solution was carried out with ultrasonic extraction. The chromatographic column was romasil C18(250 mm×4.6 mm,5 μm),the mobile phase was methanol:water(0.02% phosphoric acid added)=45∶55,the detection wavelength was 360 nm,the flow rate was0.8 m L·min-1,and the column temperature was room temperature. There was a good linear relationship between chlorogenic acid and quercetin in the range of 17.6 μg·mL~(-1)～132 μg·mL~(-1) and 2.16 μg·mL~(-1)～16.2 μg·mL~(-1),with correlation coefficients of 0.9994 and 0.9996,respectively. The average recovery was 97.3% and 98.2%,respectively(n=6),and the corresponding RSD was 1.2% and 1.6%,respectively. The method is simple and rapid,with good reproducibility and stability,and can be used for the determination of chlorogenic acid and quercetin in Mongolian medicine sendeng-4.
引文
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[17]白玉霞,巴根那,武晓兰,等.薄层-紫外法测定森登-4汤中栀子甙含量[J].时珍国医国药,1999,10(10):734-735.
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[2]许良,高扬,刘妍,等.蒙药森登-4汤抗炎镇痛有效部位及其化学成分的研究[J].时珍国医国药,2013,24(1):1-3.
[3]宋海超,白埔,董玉.蒙药森登-4有效部位化学成分分离与鉴定(Ⅱ)[J].内蒙古大学学报(自然科学版),2015,46(1):71-74.
[4]李时荣,张文涛,田滋,等.蒙药森等-4对胶原性大鼠关节炎的实验研究[J].内蒙古医科大学学报,2013,35(6):479-483.
[5]白玉霞,包宝柱,巴根那,等.正交试验法研究森登-4汤煎煮工艺[J].中国民族医药杂志,2001,7(4):22-23.
[6]许良,毕开顺.多元线性回归分析法在蒙药森登-4汤谱效关系解析中的应用[J].计算计与应用化学,2008,25(10):1189-1192.
[7]董玉,马强,武娜,等.蒙药复方森登-4不同配伍对总黄酮和杨梅素提取量的影响[J].内蒙古大学学报(自然科学版),2011,42(3):323-327.
[8]董玉,武娜,马强,等.蒙药复方森登-4不同配伍对总萜和栀子苷提取量的影响[J].内蒙古大学学报(自然科学版),2011,42(6):686-690.
[9]Liang Xu,Lei Zhang,Qing Li,et al.Determination of Geniposide in Rats Plasma.Application to Pharmacokinetic Studies of Sendeng-4 Decoction[J].Chromatographia,2006,63(9-10):493-497.
[10]王曦烨,李丹,唐兴盟,等.基于组学方法的蒙药森登-4配伍机制研究[J].中草药,2016,47(5):736-740.
[11]Xiye Wang,Dan Li,Mingyang Jiang,et al.K Nearest Neighbor algorithm coupled with metabonomics to study the therapeutic mechanism of Sendeng-4 in adjuvant-induced rheumatoid arthritis rat[J].Journal of Complementary and Alternative Medicine,2018,Article ID 2484912,9 pages,https://doi.org/10.1155/2018/2484912.
[12]许良,苏日塔拉图,刘淑琴.正交试验优化蒙药森登-4汤的乙醇提取工艺研究[J].数理医药学杂志,2009,22(4):456-458.
[13]范蕾,常福厚,韩瑞兰.森登-4胶囊的药理学作用研究[J].内蒙古医学院学报,2010,32(6):558-560.
[14]范蕾,常福厚,韩瑞兰.森登-4胶囊安全性研究[J].毒理学杂志,2010,24(3):246-248.
[15]雷清怡,白音夫,李德良,等.蒙药森登四味胶囊对大鼠长期毒性作用的研究[J].中国民族医药杂志,2008,24(3):49-50.
[16]王焕芸,鞠爱华,魏青,等.薄层扫描法测定蒙药森登四味汤散中槲皮素的含量[J].中国民族医药杂志,2007,12(12):45-46.
[17]白玉霞,巴根那,武晓兰,等.薄层-紫外法测定森登-4汤中栀子甙含量[J].时珍国医国药,1999,10(10):734-735.
[18]董玉,王爱民,陈朝军,等.蒙药复方森登-4中总黄酮含量的测定[J].内蒙古医学院学报,2008,30(6):440-442.