微弧氧化和碱处理技术在多孔钽修复兔颅骨缺损中的应用
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摘要
目的观察微弧氧化和碱处理对多孔钽表面性状、生物相容性和成骨能力的影响。方法微弧氧化和碱处理多孔钽片后,扫描电镜观察表面微孔数量、表面钙磷沉积和接触角。植入钽片修复兔颅骨缺损模型,在4周和12周观察骨愈合情况。结果扫描电镜显示处理组表面有更多的微孔和钙磷沉积以及更小的接触角(P<0. 05)。植入多孔钽片后,所有动物均生长良好,伤口愈合佳。CT观察多孔钽片和周围骨组织耦合良好;钙黄绿素标记检测显示12周时有新生骨长入多空钽材料内部;扫描电镜观察发现4周时多空钽材料内部有新生血管,12周时有骨小梁长入材料内部。结论微弧氧化和碱处理能改变多孔钽材料表面形状,处理后多孔钽片具有良好的生物相容性和成骨能力。
Objective To observe the effects of micro-arc oxidation( MAO) and alkali( Na OH) treatment on the porous surface tantalum,and evaluate the biocompatibility and osteogenic ability of porous tantalum in vivo. Methods Porous tantalum was immersed in simulated body fluid( SBF) for 1 week after MAO and alkali treatment. Micropores,calcium,and phosphorus deposition on the surface were observed by scanning electron microscopy( SEM). The contact angle was observed by X-ray photoelectron spectroscopy( XPS). For in vivo experiments,bone healing was evaluated at 4 and 12 weeks after the porous tantalum implantation into a rabbit skull defect model. Results Compared with the control and MAOonly treated specimens,Na OH-treated tantalum metal formed more apatite on its surface in SBF. Moreover,a sodium tantalate hydrogel layer and reduced contact angle were observed on the specimen surface by XPS( P < 0. 05). In vivo,the wounds healed well and there was no swelling or suppuration in the rabbit implant model. Computerized tomography imaging revealed that the porous bract and surrounding bone tissue were well coupled after 4 and 12 weeks. Calcein fluorescent labeling and detection indicated that new bone had grown into the interior of the multi-porous material at 12 weeks. Moreover,neovascularization and new trabecular bone were observed by SEM. Conclusions MAO and alkali treatment can change the surface shape of porous tantalum material,exhibiting good biocompatibility and osteogenic ability in vivo.
Objective To observe the effects of micro-arc oxidation( MAO) and alkali( Na OH) treatment on the porous surface tantalum,and evaluate the biocompatibility and osteogenic ability of porous tantalum in vivo. Methods Porous tantalum was immersed in simulated body fluid( SBF) for 1 week after MAO and alkali treatment. Micropores,calcium,and phosphorus deposition on the surface were observed by scanning electron microscopy( SEM). The contact angle was observed by X-ray photoelectron spectroscopy( XPS). For in vivo experiments,bone healing was evaluated at 4 and 12 weeks after the porous tantalum implantation into a rabbit skull defect model. Results Compared with the control and MAOonly treated specimens,Na OH-treated tantalum metal formed more apatite on its surface in SBF. Moreover,a sodium tantalate hydrogel layer and reduced contact angle were observed on the specimen surface by XPS( P < 0. 05). In vivo,the wounds healed well and there was no swelling or suppuration in the rabbit implant model. Computerized tomography imaging revealed that the porous bract and surrounding bone tissue were well coupled after 4 and 12 weeks. Calcein fluorescent labeling and detection indicated that new bone had grown into the interior of the multi-porous material at 12 weeks. Moreover,neovascularization and new trabecular bone were observed by SEM. Conclusions MAO and alkali treatment can change the surface shape of porous tantalum material,exhibiting good biocompatibility and osteogenic ability in vivo.
引文
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[2] Bratton EM,Durairaj VD. Orbital implants for fracture repair[J]. Curr Opin Ophthalmol,2011,22(5):400-406.
[3] Hefni EK,Bencharit S,Kim SJ,et al. Transcriptomic profiling of tantalum metal implant osseointegration in osteopenic patients[J]. BDJ Open,2018,4:17042.
[4]张大鹏,刘永庆,王志强.医用多孔钽的特性及其表面改性的研究进展[J].中国煤炭工业医学杂志,2017,2:113-116.Zhang DP, Liu YQ, Wang ZQ. Research advances on the properties and surface modification of medical porous tantalum[J]. Chin J of Coal Ind Med,2017,2:113-116.
[5] Lin DJ,Fuh LJ,Chen CY,et al. Rapid nano-scale surface modification on micro-arc oxidation coated titanium by microwave-assisted hydrothermal process[J]. Mater Sci Eng C Mater Biol Appl,2019,95:236-247.
[6] Huang Q,Li X,Elkhooly TA,et al. The Cu-containing Ti O2coatings with modulatory effects on macrophage polarization and bactericidal capacity prepared by micro-arc oxidation on titanium substrates[J]. Colloids Surf B Biointerfaces,2018,170:242-250.
[7]潘宜鹏,刘煜,李明,等.改良颅内加压法兔脑死亡模型建立及其状态的维持[J].中国比较医学杂志,2016,26(1):25-28.Pan YP,Liu Y,Li M,et al. Establishment of brain death model for rabbits in a modified way by increasing intracranial pressure and maintaining the state of brain death[J]. Chin J Comp Med,2016,26(1):25-28.
[8] Doonan C,Ricco R,Liang K,et al. Metal-organic frameworks at the biointerface:synthetic strategies and applications[J]. Acc Chem Res,2017,50(6):1423-1432.
[9] Kim SY, Kim YK, Kim KS, et al. Enhancement of bone formation on LBL-coated Mg alloy depending on the different concentration of BMP-2[J]. Colloids Surf B Biointerfaces,2019,173:437-446.
[10]陆雨桐,李涛,商晓盼,等.镁合金微弧氧化表面处理研究进展[J].生物医学工程学杂志,2016,33(5):1016-1019.Lu YT,Li T,Shang XP,et al. Research progress on surface treatment of magnesium alloy by micro-arc oxidation[J]. J Biomedical Eng,2016,33(5):1016-1019.
[11] Kim SY,Kim YK,Ryu MH,et al. Corrosion resistance and bioactivity enhancement of MAO coated Mg alloy depending on the time of hydrothermal treatment in Ca-EDTA solution[J]. Sci Rep,2017,7(1):9061.
[12] Hsu HC,Wu SC,Hsu SK,et al. Effect of different posttreatments on the bioactivity of alkali-treated Ti-5Si alloy[J].Biomed Mater Eng,2017,28(5):503-514.
[13] Christensen E,Nilsen V,Hkonsen T,et al. Removal of model viruses,E. coli and Cryptosporidium oocysts from surface water by zirconium and chitosan coagulants[J]. J Water Health,2017,15(5):695-705.
[14] Kim C,Kendall MR,Miller MA,et al. Comparison of titanium soaked in 5 M Na OH or 5 M KOH solutions[J]. Mater Sci Eng C Mater Biol Appl,2013,33(1):327-339.
[15] Gad Allah AG,Badawy WA,Rehan HH. Stability of anodic tantalum oxide films in Na OH solutions[J]. J Appl Electrochem,1989,19(5):768-776.
[16] Li SJ,Yang R,Niinomi M,et al. Formation and growth of calcium phosphate on the surface of oxidized Ti-29Nb-13Ta-4. 6Zr alloy[J]. Biomaterials,2004,25(13):2525-2532.
[17] Whitcroft KL,Merkonidis C,Cuevas M,et al. Intranasal sodium citrate solution improves olfaction in post-viral hyposmia[J].Rhinology,2016,54(4):368-374.
[18] Alhalawani AM,Mehrvar C,Stone W,et al. A novel tantalumcontaining bioglass. Part II. Development of a bioadhesive for sternal fixation and repair[J]. Mater Sci Eng C Mater Biol Appl,2017,71:401-411.
[19] Liu Y,Ji X,Liu J,et al. Tantalum sulfide nanosheets as a theranostic nanoplatform for computed tomography imagingguided combinatorial chemo-photothermal therapy[J]. Adv Funct Mater,2017,27(39). pii:1703261.
[20] Miyazaki T,Kim HM,Kokubo T,et al. Enhancement of bonding strength by graded structure at interface between apatite layer and bioactive tantalum metal[J]. J Mater Sci Mater Med,2002,13(7):651-655.
[21] Capellato P,Escada AL,Popat KC,et al. Interaction between mesenchymal stem cells and Ti-30Ta alloy after surface treatment[J]. J Biomed Mater Res A,2014,102(7):2147-2156.
[22]李琪佳,王茜,甘洪全,等.多孔钽材料细胞毒性、生物相容性及体内成骨性研究[J].中华骨科杂志,2014,34(9):954-961. Li QJ, Wang Q, Gan HQ, et al. The cytotoxicity,biocompatibility and morphological observation of bone formation traced in porous tantalum[J]. Chin J Orthop,2014,34(9):954-961.