超声辅助酶法合成异丙酯的研究
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摘要
文章以茶油为原料,利用超声辅助酶法合成了异丙酯。考察了超声功率、超声预处理时间、酶添加量、底物比和反应温度对异丙酯得率的影响。通过响应面确定了最优工艺条件为超声功率200 W、超声预处理时间5 min、酶添加量220 U/g、底物比6∶1、反应温度60℃,在此条件下反应6 h异丙酯得率在93.64%以上;同时Novozym 435脂肪酶在最佳条件下反应10批次后仍有较高的稳定性。
In this paper, isopropyl ester was synthesized by ultrasonic assisted enzymatic method using camellia oil as raw materials. The effects of ultrasonic power, ultrasonic pretreatment time, enzyme dosage, substrate ratio and reaction temperature on the yield of isopropyl ester were researched. The optimal process conditions were determined by response surface methodology: ultrasonic power was 200 W, ultrasonic pretreatment time was 5 min, enzyme dosage was 220 U/g, substrate ratio was 6∶1 and reaction temperature was 60 ℃. Under the above conditions, the yield of isopropyl ester was above 93.64% after reaction for 6 h. At the same time, Novozym 435 lipase had higher stability under the optimal conditions for the reaction of 10 batches.
In this paper, isopropyl ester was synthesized by ultrasonic assisted enzymatic method using camellia oil as raw materials. The effects of ultrasonic power, ultrasonic pretreatment time, enzyme dosage, substrate ratio and reaction temperature on the yield of isopropyl ester were researched. The optimal process conditions were determined by response surface methodology: ultrasonic power was 200 W, ultrasonic pretreatment time was 5 min, enzyme dosage was 220 U/g, substrate ratio was 6∶1 and reaction temperature was 60 ℃. Under the above conditions, the yield of isopropyl ester was above 93.64% after reaction for 6 h. At the same time, Novozym 435 lipase had higher stability under the optimal conditions for the reaction of 10 batches.
引文
[1] 聂海瑜.油茶籽的综合利用[J].粮油加工与食品机械,2004(6):39-41.
[2] 庄瑞林.中国茶油[M].2版.北京:中国林业出版社,2008.
[3] 李丽,吴雪辉,寇巧花.茶油的研究现状及应用前景[J].中国油脂,2010,35(3):10-13.
[4] 左伟英,杨旸,邱礼鸿.油茶籽油紫外光吸收特性分析及应用[J].粮油食品科技,2013,21(3):47-49.
[5] DESHMANE V G,GOGATE P R,PANDIT A B.Ultrasound assisted synthesis of isopropyl esters from palm fatty acid distillate[J].Ultrasonics sonochemistry,2009,16(3):345-350.
[6] 倪永全.脂肪酸异丙酯的制取、性能及应用[J].日用化学工业,1988(1):26-28.
[7] LIU Y,TAN H,ZHANG X,et al.Effect of monohydric alcohols on enzymatic transesterification for biodiesel production[J].Chemical Engineering Journal,2010,157(1):223-229.
[8] 陈小威,孙尚德,毕艳兰,等.无溶剂体系超声协同脂肪酶催化合成阿魏酸酰基甘油的初步探讨[J].食品科技,2013 (4):281-285.
[9] 王建勋,黄庆德,黄凤洪,等.超声波辅助下脂肪酶催化高酸值废油脂制备生物柴油[J].生物工程学报,2007,23(6):1121-1128.
[10] LEE S H,NGUYEN H M,KOO Y M,et al.Ultrasound-enhanced lipase activity in the synthesis of sugar ester using ionic liquids[J].Process Biochemistry,2008,43(9):1009-1012.
[11] 廖凯波,曾虹燕,邓欣,等.微水相超声波协同固定化脂肪酶催化酯交换过程优化[J].天然产物研究与开发,2009(5):871-874.
[2] 庄瑞林.中国茶油[M].2版.北京:中国林业出版社,2008.
[3] 李丽,吴雪辉,寇巧花.茶油的研究现状及应用前景[J].中国油脂,2010,35(3):10-13.
[4] 左伟英,杨旸,邱礼鸿.油茶籽油紫外光吸收特性分析及应用[J].粮油食品科技,2013,21(3):47-49.
[5] DESHMANE V G,GOGATE P R,PANDIT A B.Ultrasound assisted synthesis of isopropyl esters from palm fatty acid distillate[J].Ultrasonics sonochemistry,2009,16(3):345-350.
[6] 倪永全.脂肪酸异丙酯的制取、性能及应用[J].日用化学工业,1988(1):26-28.
[7] LIU Y,TAN H,ZHANG X,et al.Effect of monohydric alcohols on enzymatic transesterification for biodiesel production[J].Chemical Engineering Journal,2010,157(1):223-229.
[8] 陈小威,孙尚德,毕艳兰,等.无溶剂体系超声协同脂肪酶催化合成阿魏酸酰基甘油的初步探讨[J].食品科技,2013 (4):281-285.
[9] 王建勋,黄庆德,黄凤洪,等.超声波辅助下脂肪酶催化高酸值废油脂制备生物柴油[J].生物工程学报,2007,23(6):1121-1128.
[10] LEE S H,NGUYEN H M,KOO Y M,et al.Ultrasound-enhanced lipase activity in the synthesis of sugar ester using ionic liquids[J].Process Biochemistry,2008,43(9):1009-1012.
[11] 廖凯波,曾虹燕,邓欣,等.微水相超声波协同固定化脂肪酶催化酯交换过程优化[J].天然产物研究与开发,2009(5):871-874.