别直参抑制人乳腺癌细胞MCF-7体外增殖实验研究
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摘要
[目的]评价别直参对MCF-7细胞体外增殖的影响,并检测其对凋亡相关蛋白bax/bcl-2表达的影响。[方法]采用MTT比色法检测对MCF-7细胞增殖的作用;流式细胞术检测对MCF-7细胞凋亡的影响;免疫组化法检测bax/bcl-2的表达。[结果]别直参高剂量组作用MCF-7细胞24h后,MTT检测增殖率为89.83%,提示有抑制MCF-7细胞体外增殖的作用(P<0.05);流式细胞术检测高剂量组细胞凋亡率明显升高(P<0.05);免疫组化法检测高剂量组能上调MCF-7细胞bax的表达,并下调bcl-2的表达。[结论]别直参高剂量组具有一定的诱导MCF-7细胞凋亡效应作用,其机制可能通过上调bax的表达和下调bcl-2的表达。
[Purpose] To investigate the impact of serum containing Korean Ginseng on MCF-7 cell proliferation and the expression of bax/bal-2 proteins. [Methods] By using the MTT method to detect cell proliferation and flow cytometry to examine cell apoptosis,while the bax/bcl-2 proteins were detected by IHC. [Results] The high dose of Korean ginseng could make an anti-proliferative effect on MCF-7 cell with the proliferation rate of 89.83% by MTT method as well as high apoptosis rate by flow cytometry(P<0.05). The IHC revealed high dose of Korean ginseng increase the expression of bax protein and decrease the expression of bcl-2 protein. [Conclusion] The high dose of Korean ginseng induced apoptosis in MCF-7 cell by increasing the expression of bax protein and decreasing the expression of bcl-2 protein.
[Purpose] To investigate the impact of serum containing Korean Ginseng on MCF-7 cell proliferation and the expression of bax/bal-2 proteins. [Methods] By using the MTT method to detect cell proliferation and flow cytometry to examine cell apoptosis,while the bax/bcl-2 proteins were detected by IHC. [Results] The high dose of Korean ginseng could make an anti-proliferative effect on MCF-7 cell with the proliferation rate of 89.83% by MTT method as well as high apoptosis rate by flow cytometry(P<0.05). The IHC revealed high dose of Korean ginseng increase the expression of bax protein and decrease the expression of bcl-2 protein. [Conclusion] The high dose of Korean ginseng induced apoptosis in MCF-7 cell by increasing the expression of bax protein and decreasing the expression of bcl-2 protein.
引文
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[2]Xie CS,Xu LJ.Effect of serum containing radix Ginseng Rubra on cell proliferation and cell cycle in human breast cancer cell line MCF-7[J].China Cancr,2013,22(4):291-294.[谢长生,徐丽君.红参含药血清对人乳腺癌MCF-7细胞增殖和细胞周期的影响[J].中国肿瘤,2013,22(4):291-294.]
[3]Zhu CY,Zhang X,Ye LH.Advance on antitumor activity of ginsenoside Rb[J].Acta Chin Med Pharmacol,2014,42(2):81-86.[朱春燕,张旭,叶丽红.人参皂苷Rd的抗癌机制研究进展[J].中医药学报,2014,42(2):81-86.]
[4]Yang QY,Li XY,Liu GL.Research progress in pharmacological activities of Ginsenoside Rb1[J].Chin Pharm J,2013,48(15):1233-1237.[杨秋娅,李晓宇,刘皋林.人参皂苷Rb1的药理作用研究进展[J].中国药学杂志,2013,48(15):1233-1237.]
[5]Feng XN,Jang G,Li Q.Ginsenoside Rh2 sensitized 5-fluorouracil which induced human breast cancer MCF-7apoptosis[J].Journal of Shenyang Pharmaceutical University,2012,29(5):383-389.[冯晓娜,蒋革,李清.人参皂苷Rh2提高人乳腺癌细胞MCF-7对5-氟尿嘧啶诱导凋亡的敏感性[J].沈阳药科大学学报,2012,29(5):383-389.]
[6]Chen Y,Zhang B,Yu Y,et al.Effect of ginsenoside Rg3 on cell proliferation and invasion of breast cancer MCF-7 cell line[J].Chin JMAP,2013,30(7):722-725.[陈云,张竝,俞勇,等.人参皂苷Rg3对乳腺癌MCF-7细胞增殖和侵袭的影响[J].中国现代应用药学,2013,30(7):722-725.]
[7]Li GD,Yang H,Lu Y.Effects of ginsenoside Rg3 on vasculogenic mimicry of breast cancer MCF-7 cell line[J].Tianjin Med J,2016,44(9):1069-1072.[黎国栋,杨慧,陆颖.人参皂苷Rg3对人乳腺癌MCF-7细胞血管生成拟态的影响[J].天津医药,2016,44(9):1069-1072.]
[8]Xie XD,Zhu YK.Effects of ginsenoside on VEGF in gastric cancer patients with chemotherapy after surgery[J].Chinese Journal of Surgical Oncology,2014,6(3):149-152.[谢晓东,朱永康.人参皂苷对胃癌术后化疗患者血清VEGF的影响[J].中国肿瘤外科杂志,2014,6(3):149-152.]
[9]Yin HF,Xiu YC,Zhao WM,et al.Effects of ginsenoside Rg3on prostate cancer cells[J].Modern Medical Journal,2016,44(1):1-6.[尹怀福,修有成,赵维明,等.人参皂甙Rg3对前列腺癌细胞的作用研究[J].现代医学,2016,44(1):1-6.]
[10]Jian J,Liu LZ,Huang Y,et al.Effect of Ginsenoside Rg3on cell proliferation of human colon cancer cell line SW480 and its mechanism[J].Shan Dong Med,2016,56(3):8-10.[简捷,刘利珍,黄缘,等.人参皂苷Rg3对人结肠癌细胞株SW480增殖的影响及其作用机制[J].山东医药,2016,56(3):8-10.]
[11]Wang H,Liu S.Initial research on inhibitory effect of Ginsenoside CK on lung adenocarcinoma A549 cell line proliferation and its mechanism[J].Modern Oncology,2016,24(4):534-537.[王红,刘珊.人参皂苷CK对人肺腺癌A549细胞株的增殖抑制作用及其机制[J].现代肿瘤医学,2016,24(4):534-537.]
[12]Lee DG,Jang SI,Kim YR.Anti-proliferative effects of Ginsenosides extracted from mountain ginseng on lung cancer[J].Chin J Inteqr Med,2016,22(5):344-352.
[13]Rao YQ,Liu XE,Wang YX,et al.Effect of Ginsenoside Rg3 on cell proliferation of human lung cancer cell line NCI-H1650[J].Journal of Chinese Oncology,2013,19(6):413-416.[饶远权,刘杏娥,王廷祥,等.人参皂苷Rg3抑制肺癌NCI-H1650细胞增殖作用研究[J].肿瘤学杂志,2013,19(6):413-416.]