miRNA-374a在狼疮肾炎患者血清中的表达及其意义
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摘要
目的探讨狼疮肾炎患者血清miRNA-374a的表达情况及其意义。方法运用Target Scan靶基因预测分析软件等生物信息学方法,预测miRNA-374a靶基因并进行分析;采用qRT-PCR方法检测miRNA-374a、MCP-1在狼疮肾炎患者血清和健康对照组血清的表达变化,并分析其与临床参数的关系。结果生物信息学分析显示MCP-1为狼疮肾炎的靶基因;与健康对照组相比,miRNA-374a在狼疮肾炎患者血清低表达(P<0.05),MCP-1在狼疮肾炎患者血清高表达(P<0.05);miRNA-374a、MCP-1的表达水平与年龄没有明显关系(P>0.05),与临床分期有关(P<0.05)。结论 miRNA-374a在狼疮肾炎患者血清中低表达,可能通过对其下游靶基因MCP-1的调控,在狼疮肾炎发生过程中发挥作用。
Objective To investigate serum mi RNA-374 a expression in patients with lupus nephritis and its clinical significance. Methods Target genes of mi RNA-374 a were predicted by bioinformatics methods such as Target Scan and closely related gene with lupus nephritis were screened. The expression levels of serum mi RNA-374 a and MCP-1 in patients with lupus nephritis and healthy controls were detected using q RT-PCR,and the correlations between those and clinical parameters were analyzed. Results Bioinformatics analysis revealed that of mi RNA-374 a target genes was MCP-1 in lupus nephritis. Compared with healthy controls,serum mi RNA-374 a was significantly lowly expressed in patients with lupus nephritis while MCP-1 was significantly highly expressed(P<0.05);expression levels of mi RNA-374 a and MCP-1 showed no significant correlation with age(P>0.05),but with clinical stages(P<0.05). Conclusion Low expression of mi RNA-374 a was detected in the serum of patients with lupus nephritis. It may play an important role in the pathogenesis and progression of lupus nephritis by regulating the downstream target gene MCP-1.
Objective To investigate serum mi RNA-374 a expression in patients with lupus nephritis and its clinical significance. Methods Target genes of mi RNA-374 a were predicted by bioinformatics methods such as Target Scan and closely related gene with lupus nephritis were screened. The expression levels of serum mi RNA-374 a and MCP-1 in patients with lupus nephritis and healthy controls were detected using q RT-PCR,and the correlations between those and clinical parameters were analyzed. Results Bioinformatics analysis revealed that of mi RNA-374 a target genes was MCP-1 in lupus nephritis. Compared with healthy controls,serum mi RNA-374 a was significantly lowly expressed in patients with lupus nephritis while MCP-1 was significantly highly expressed(P<0.05);expression levels of mi RNA-374 a and MCP-1 showed no significant correlation with age(P>0.05),but with clinical stages(P<0.05). Conclusion Low expression of mi RNA-374 a was detected in the serum of patients with lupus nephritis. It may play an important role in the pathogenesis and progression of lupus nephritis by regulating the downstream target gene MCP-1.
引文
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[12]Wada T,Furuichi K.MIP-1 alpha and MCP-1 contribute to crescents Andinters-titial lesions in human crescentic glomerulonephritis[J].Kidney Int,1999,56:995-1003.
[13]Dai C,Liu Z,Zhou H,et al.Monocyte chemoattractant protein-1expression inrenal tissue isassociated with monocyte recruitment and Tubulointer-stitial lesions inpatients with lupus nephritis[J].Chin Med J(Engl),2001,114:864-886.
[14]刘中娟,林嘉友.单核细胞趋化蛋白-1表达与狼疮肾炎[J].中国医学科学院学报,2005,27(4):491-495.
[15]陈泉池,贾成友,余飞,等.微小RNA-374a抑制乳腺癌细胞增殖和迁移能力[J].中华实验外科杂志,2014,31(2):292-294.
[2]杨阳,张运津,崔苏萍,等.micro RNAs在肾透明细胞癌组织中的表达及意义[J].实验与检验医学,2012,30(2):119-122.
[3]陈钦开,王满庭,杨柳,等.多囊肾患者家系血清mi RNA-15a和mi RNA-17表达水平的比较[J].实验与检验医学,2011,29(1):1-2.
[4]吴珏,刘芬,阮琼芳,等.mi RNA-92a在缺血再灌注肾损伤中的表达变化[J].实验与检验医学,2010,28(1):9-11.
[5]殷俊辉,张长青,牛鑫,等.mi RNA-210在股骨头坏死患者血浆中的表达变化[J].实验与检验医学,2012,30(1):13-14.
[6]杨阳,张运津,谢安,等.糖尿病肾病患者血清mi RNA-93的表达变化及意义[J].实验与检验医学,2012,30(3):222-224.
[7]杨阳,张成,娄远蕾,等.前列腺癌患者血清mi RNA-192的表达及其靶基因预测分析[J].广东医学,2012,33(15):2259-2261.
[8]Vosa U,Vooder T,Kolde R,et al.Identification of mi R-374a as a prognostic marker for survival in patients with early-stage nonsmall cell lung cancer[J].Genes Chromosomes Cancer,2011,50(10):812-22.
[9]田瑞,李建一,张文海,等.mi RNA-374a在乳腺癌侵袭与转移中的作用及机制[J].中华内分泌外科杂志,2014,8(1):53-56,61.
[10]Li JY,Zhang Y,Zhang WH,et al.Effects of differential distribution of microvessel density,possibly regulated by mi R-374a,on breast cancer prognosis[J].Asian Pac J Cancer Prev,2013,14(3):1715-1720.
[11]Cai J,Guan H,Fang L,et al.Micro RNA-374a activates Wnt/β-catenin signaling to promote breast cancer metastasis[J].J Clin Invest,2013,123(2):566-779.
[12]Wada T,Furuichi K.MIP-1 alpha and MCP-1 contribute to crescents Andinters-titial lesions in human crescentic glomerulonephritis[J].Kidney Int,1999,56:995-1003.
[13]Dai C,Liu Z,Zhou H,et al.Monocyte chemoattractant protein-1expression inrenal tissue isassociated with monocyte recruitment and Tubulointer-stitial lesions inpatients with lupus nephritis[J].Chin Med J(Engl),2001,114:864-886.
[14]刘中娟,林嘉友.单核细胞趋化蛋白-1表达与狼疮肾炎[J].中国医学科学院学报,2005,27(4):491-495.
[15]陈泉池,贾成友,余飞,等.微小RNA-374a抑制乳腺癌细胞增殖和迁移能力[J].中华实验外科杂志,2014,31(2):292-294.