一种由可可毛色二孢引起的春芋新病害
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摘要
<正>可可毛色二孢(Lasiodiplodia theobromae(Pat.) Griffon&Maubl.)是由Griffon和Maublanc~([1])在对Botryodiplodia theobromae进行订正的基础上而确立,Alves等~([2])于2008年对这个种又进行了研究并补充了DNA序列信息。可可毛色二孢是一种重要的病原菌,可引起多种植物的枯萎、根腐及焦枯等病害症状~([3,4])。而假可可毛色二
Two fungal isolates were isolated from diseased leaves of Philodendron selloum in Guangxi medicinal garden,Nanning,Guangxi. It indicated that they should belong to Lasiodiplodia based on morphological observation,and they were very similar to Lasiodiplodia theobromae after further comparison in morphology.Meanwhile,the multi-gene phylogenetic tree based on the DNA sequences of ITS,β-tubulin and elogation factor( EF-α) of isolates collected in this study and the ex-type strain of L. theobromae was built up. The results also supported that these two fungi were L. theobromae. The test of Koch's rule confirmed that L. theobromae was the pathogen of this disease. This is the first report about L. theobromae causing P. selloum disease in China.
Two fungal isolates were isolated from diseased leaves of Philodendron selloum in Guangxi medicinal garden,Nanning,Guangxi. It indicated that they should belong to Lasiodiplodia based on morphological observation,and they were very similar to Lasiodiplodia theobromae after further comparison in morphology.Meanwhile,the multi-gene phylogenetic tree based on the DNA sequences of ITS,β-tubulin and elogation factor( EF-α) of isolates collected in this study and the ex-type strain of L. theobromae was built up. The results also supported that these two fungi were L. theobromae. The test of Koch's rule confirmed that L. theobromae was the pathogen of this disease. This is the first report about L. theobromae causing P. selloum disease in China.
引文
[1]Griffon E,Maublanc A,Sur une maladie du cacaoyer.Bulletin de la SociétéM ycologique de France[J],1909,25(1):51-58
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[3]Sreerama K P,Singh L.Lasiodiplodia theobromae is a M ycoparasite of a Pow dery M ildew Pathogen[J].M ycobiology,2009,37(4):308-309.
[4]Pedraza J M T,Aguilera J A M,Díaz C N,et al.Control of Lasiodiplodia theobromae,the causal agent of dieback of sapote mamey[Pouteria sapota(Jacq.)H.E.M oore and Stearn]Grafts in M exio[J].Revista Fitotecnia M exicana Publ Por La Sociedad M exicana De Fitogene'tica,2013,36(3):233-238.
[5]Wang J N,Zhao H H,Yu Y Y,et al.The pathogen causing Lasiodiplodia tw ig blight of bluseberry(in Chinese)[J].Mycosystema(菌物学报),2016,35(3):1-9.
[6]Kwon J H,Choi O,Kang B,et al.Identification of Lasiodiplodia pseudotheobromae causing mango dieback in Korea[J].Canadian Journal of Plant Pathology,2017,39(2):241-245.
[7]Wee J I,Back C G,Park M J,et al.First report of die-back on rose(Rosa hybrida)caused by Lasiodiplodia pseudotheobromae in Korea[J].Research in Plant Disease,2017,23(4):367-371.
[8]Chomnunti P,Hongsanan S,Aguirre-Hudson B,et al.The sooty moulds[J].Fungal Diversity,2014,66(1):1-36.
[9]White T J,Bruns T,Lee S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies[A].Innis M A,Gelfand D H,Sninsky JJ,et al.PCR protocols:A guide to methods and applications[M].San Diego:Academic Press,1990:315-322.
[10]Glass N L,Donaldson G C.Development of primer sets designed for use w ith the PCR to amplify conserved genes from filamentous ascomycetes[J].Applied and Environmental M icrobiology,1995,61(4):1323-1330.
[11]Carbone I,Kohn L.A method for designing primer sets for speciation studies in filamentous ascomycetes[J].Mycologia,1999,91(3):553-556.
[12]Yang T,Groenewald J Z,Cheewangkoon R,et al.Families,genera,and species of Botryosphaeriales[J].Fungal Biology,2017,121(4):322-346.
[13]Swofford,DL.PAUP*.Phylogenetic Analysis Using Parsimony(*and other methods)Version 4[M].Sunderland,M assachusetts:Sinauer Associates,2003.
[14]Dissanayake A J,Zhang W,Liu M,et al.Lasiodiplodia pseudotheobromae causes pedicel and peduncle discolouration of grapes in China[J].Australasian Plant Disease Notes,2015,10(1):1-5.
[2]Alves A,Crous P W,Correia A,et al.Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae[J].Fungal Diversity,2008,28(2):1-13.
[3]Sreerama K P,Singh L.Lasiodiplodia theobromae is a M ycoparasite of a Pow dery M ildew Pathogen[J].M ycobiology,2009,37(4):308-309.
[4]Pedraza J M T,Aguilera J A M,Díaz C N,et al.Control of Lasiodiplodia theobromae,the causal agent of dieback of sapote mamey[Pouteria sapota(Jacq.)H.E.M oore and Stearn]Grafts in M exio[J].Revista Fitotecnia M exicana Publ Por La Sociedad M exicana De Fitogene'tica,2013,36(3):233-238.
[5]Wang J N,Zhao H H,Yu Y Y,et al.The pathogen causing Lasiodiplodia tw ig blight of bluseberry(in Chinese)[J].Mycosystema(菌物学报),2016,35(3):1-9.
[6]Kwon J H,Choi O,Kang B,et al.Identification of Lasiodiplodia pseudotheobromae causing mango dieback in Korea[J].Canadian Journal of Plant Pathology,2017,39(2):241-245.
[7]Wee J I,Back C G,Park M J,et al.First report of die-back on rose(Rosa hybrida)caused by Lasiodiplodia pseudotheobromae in Korea[J].Research in Plant Disease,2017,23(4):367-371.
[8]Chomnunti P,Hongsanan S,Aguirre-Hudson B,et al.The sooty moulds[J].Fungal Diversity,2014,66(1):1-36.
[9]White T J,Bruns T,Lee S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies[A].Innis M A,Gelfand D H,Sninsky JJ,et al.PCR protocols:A guide to methods and applications[M].San Diego:Academic Press,1990:315-322.
[10]Glass N L,Donaldson G C.Development of primer sets designed for use w ith the PCR to amplify conserved genes from filamentous ascomycetes[J].Applied and Environmental M icrobiology,1995,61(4):1323-1330.
[11]Carbone I,Kohn L.A method for designing primer sets for speciation studies in filamentous ascomycetes[J].Mycologia,1999,91(3):553-556.
[12]Yang T,Groenewald J Z,Cheewangkoon R,et al.Families,genera,and species of Botryosphaeriales[J].Fungal Biology,2017,121(4):322-346.
[13]Swofford,DL.PAUP*.Phylogenetic Analysis Using Parsimony(*and other methods)Version 4[M].Sunderland,M assachusetts:Sinauer Associates,2003.
[14]Dissanayake A J,Zhang W,Liu M,et al.Lasiodiplodia pseudotheobromae causes pedicel and peduncle discolouration of grapes in China[J].Australasian Plant Disease Notes,2015,10(1):1-5.