高粱种质资源对黑束病的抗性鉴定与评价
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摘要
2016-2017年,在人工接种条件下对110份高粱种质资源进行了抗黑束病鉴定与评价研究,拟探明高粱抗病性及不同寄主来源的高粱、玉米黑束病菌致病力差异。110份高粱种质资源中,对高粱来源的黑束病菌(GHS-1)表现免疫(IM)的13份(占11.8%),高抗(HR)的17份(占15.5%),抗病(R)和中抗(MR)的各14份(占25.4%);感病(S)的17份(占15.5%),高感(HS)的35份(占31.8%);对玉米来源的黑束病菌(YHS-1)表现免疫(IM)的21份(占19.1%),高抗(HR)的11份(占10.0%),抗病(R)的15份(占13.6%),中抗(MR)的12份(占10.9%);感病(S)的21份(占19.1%),高感(HS)的30份(占27.3%)。上述结果表明,目前高粱育种中广泛应用的育种种质恢复系中抗黑束病材料较为丰富,且不同寄主来源的高粱和玉米黑束病菌致病力存在差异。
In order to determine the resistance difference of sorghum germplasm and the pathogenicity difference of Sarocladium strictum from sorghum and maize,a total of 110 sorghum germplasm accessions were subjected for evaluation for the resistance to Black Bundle Disease under arti?cial inoculation conditions from2016 to 2017. With this collection,13 lines(11.8%)were scored as immune(IM),17 lines(15.5%)were highly resistant(HR),14 lines(12.7%)were resistant(R)and 14 lines(12.7%)were moderately resistant(MR),17 lines(15.5%)were susceptible(S),35 lines(31.8%)were highly susceptible(HS)to GHS-1. Twenty-one lines(19.1%)were immune(IM),11 lines(10.0%)were highly resistance(HR),15 lines(13.6%)were resistance(R),12 lines(10.9%)were moderately resistance(MR),21 lines(19.1%)were susceptibility(S)and 30 lines(27.3%)with highly susceptibility(HS)to YHS-1. These results showed that a proportion of restorer lines widely used in sorghum breeding were resistant to Black Bundle Disease,and the pathogenicity of Black Bundle Disease pathogens isolated from sorghum and maize was different.
In order to determine the resistance difference of sorghum germplasm and the pathogenicity difference of Sarocladium strictum from sorghum and maize,a total of 110 sorghum germplasm accessions were subjected for evaluation for the resistance to Black Bundle Disease under arti?cial inoculation conditions from2016 to 2017. With this collection,13 lines(11.8%)were scored as immune(IM),17 lines(15.5%)were highly resistant(HR),14 lines(12.7%)were resistant(R)and 14 lines(12.7%)were moderately resistant(MR),17 lines(15.5%)were susceptible(S),35 lines(31.8%)were highly susceptible(HS)to GHS-1. Twenty-one lines(19.1%)were immune(IM),11 lines(10.0%)were highly resistance(HR),15 lines(13.6%)were resistance(R),12 lines(10.9%)were moderately resistance(MR),21 lines(19.1%)were susceptibility(S)and 30 lines(27.3%)with highly susceptibility(HS)to YHS-1. These results showed that a proportion of restorer lines widely used in sorghum breeding were resistant to Black Bundle Disease,and the pathogenicity of Black Bundle Disease pathogens isolated from sorghum and maize was different.
引文
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[4]Teixeira H, Machado J D C, Oride D, Alves M C, Noda A.Water restriction technique:effect on acremonium strictum,seeds protusion and obtaining of maize seeds infected.Fitopatologia Brasileira, 2005,30(2):109-114
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[7]Stephen K N, Vibeke L, Ibrahim D K A, Francis K T. Grain mold fungi from sorghum in ghana-global conference on sorghum. Millets Diseases, 2003:227-228
[8] Lin S F, Hwangl Y L, Tsai Y C. Mmobilization of glucooligosaccharide oxidase of acremonium strictum for oligosaccharic acids production. Biotechnology Techniques,1996, 10(1):63-68
[9]Know H K, Woo S H, Park J M. Thiocyanate degradation by acremonium strictum and inhibition by secondary toxicants.Biotechnology Letters, 2002,24(16):1347-1351
[10] Tagnel A, Neergaard E, Hansen H J. The studies of hostpathogen interaction between maize and acremonium strictum from cameroon. European Journal of Plant Pathology, 2002,108:93-102
[11]姜钰,徐婧,徐秀德,胡兰·高粱三系抗丝黑穗病鉴定与评价·植物遗传资源学报,2015, 16(2):417-421Jiang Y, Xu J, Xu X D, Hu L. Identification and evaluation of sorghum male-sterile, maintainer and restorer lines resistance tohead smut. Journal of Plant Genetic Resources, 2015, 16(2):417-421
[12] Domsch D K H, Gams W, Anderson T H-Compendium of soil fungi. Vol.Ⅰ.london:Academic Press, 1980, 895
[13]徐秀德,刘志恒·高粱病虫害原色图鉴·北京:中国农业科学技术出版社,2012:43-46Xu X D, Liu Z H. A color atlas of sorghum diseases and pests.Beijing:China Agriculture Scientech Press, 2012:43-46
[14] Garcia J J, Velazquze M T, Jorda C-Acremonium species as the causal agent of muskmelon collapse in Spain-Plant Disease,1994,78:416-419
[15]胡兰,姜钰,刘航,冯连第,刘可杰,秦培文,徐婧,张明会,毕英杰,董怀玉,徐秀德,张国志. DB 21/T2790高粱抗黑束病鉴定技术规程·沈阳:辽宁质量技术监督局,2017Hu L, Jiang Y, Liu H, Feng L D, Liu K J, Qin P W, Xu J,Zhang M H, Bi Y J, Dong H Y, Xu X D, Zhang G Z-DB 21/T2790 Rule for evaluation of sorghum resistance to acremonium wilt. Shenyang:Liaoning the Quality and Technology Supervision Bureau,2017
[16] Martinez-Culebras P V, Querol A, Suarez-Fernandez M B,Garcia-Lopez M D, Barrio E-Phylogenetic relationshop among Colletotrichun pathogens of strawberry and design of PCR primers for their identification. Phytopathology, 2003, 151:135-143
[17]王晓鸣·六种重要玉米病害病原名称的厘定·中国农业科学,2018, 51(18):3497-3507Wang X M. Collation of scientific names of six maize disease pathogens. Scientia Agricultura Sinica, 2018, 51(18):3497-3507
[18] Novicki T J, Lafe K, Bui L, Bui U, Geise R, Marr K, Cookson B T-Genetic diversity among clinical isolates of acremonium strictum determined during an investigation of a fatal mycosis.Journal of Clinical Microbiology, 2003,41(6):2623-2628
[19] Summerbell R C, Gueidan C, Schroers H J, Dehoog G S,Starink M, Rosete Y A, Guarro J, Scott J A-Acremonium phylogenetic overview and revision of gliomastix, sarocladium,and trichothecium-Studies in Mycology, 2011,68:139-162
[2]Bandyopadhyay R, Mughogho L K, Satyanarayana M V Systemic infection of sorghum by acremonium strictum and its transmission through seed. Plant Disease, 1987, 71(7):647-650
[3]Teixeira H, Machado J D C. Transmissibility and effect of acremonium strictum in maize seeds. Cie ncia E Agrotecnologia,2003,27(5):1045-1052
[4]Teixeira H, Machado J D C, Oride D, Alves M C, Noda A.Water restriction technique:effect on acremonium strictum,seeds protusion and obtaining of maize seeds infected.Fitopatologia Brasileira, 2005,30(2):109-114
[5]Sener A G, Yucesoy M, Senturkun S, Afsar I, Yurtsever S G,Turk M. A case of acremonium strictum peritonitis. Medical Mycology, 2008,46(5):495
[6]徐秀德,赵廷昌,刘志恒·我国高粱上一种新病害——黑束病的初步研究·植物保护学报,1995,22(2):123-128Xu X D, Zhao T C, Liu Z H. Study on acremonium wilt in sorghum-Acta Phytophylacica Sinica, 1995,22(2):123-128
[7]Stephen K N, Vibeke L, Ibrahim D K A, Francis K T. Grain mold fungi from sorghum in ghana-global conference on sorghum. Millets Diseases, 2003:227-228
[8] Lin S F, Hwangl Y L, Tsai Y C. Mmobilization of glucooligosaccharide oxidase of acremonium strictum for oligosaccharic acids production. Biotechnology Techniques,1996, 10(1):63-68
[9]Know H K, Woo S H, Park J M. Thiocyanate degradation by acremonium strictum and inhibition by secondary toxicants.Biotechnology Letters, 2002,24(16):1347-1351
[10] Tagnel A, Neergaard E, Hansen H J. The studies of hostpathogen interaction between maize and acremonium strictum from cameroon. European Journal of Plant Pathology, 2002,108:93-102
[11]姜钰,徐婧,徐秀德,胡兰·高粱三系抗丝黑穗病鉴定与评价·植物遗传资源学报,2015, 16(2):417-421Jiang Y, Xu J, Xu X D, Hu L. Identification and evaluation of sorghum male-sterile, maintainer and restorer lines resistance tohead smut. Journal of Plant Genetic Resources, 2015, 16(2):417-421
[12] Domsch D K H, Gams W, Anderson T H-Compendium of soil fungi. Vol.Ⅰ.london:Academic Press, 1980, 895
[13]徐秀德,刘志恒·高粱病虫害原色图鉴·北京:中国农业科学技术出版社,2012:43-46Xu X D, Liu Z H. A color atlas of sorghum diseases and pests.Beijing:China Agriculture Scientech Press, 2012:43-46
[14] Garcia J J, Velazquze M T, Jorda C-Acremonium species as the causal agent of muskmelon collapse in Spain-Plant Disease,1994,78:416-419
[15]胡兰,姜钰,刘航,冯连第,刘可杰,秦培文,徐婧,张明会,毕英杰,董怀玉,徐秀德,张国志. DB 21/T2790高粱抗黑束病鉴定技术规程·沈阳:辽宁质量技术监督局,2017Hu L, Jiang Y, Liu H, Feng L D, Liu K J, Qin P W, Xu J,Zhang M H, Bi Y J, Dong H Y, Xu X D, Zhang G Z-DB 21/T2790 Rule for evaluation of sorghum resistance to acremonium wilt. Shenyang:Liaoning the Quality and Technology Supervision Bureau,2017
[16] Martinez-Culebras P V, Querol A, Suarez-Fernandez M B,Garcia-Lopez M D, Barrio E-Phylogenetic relationshop among Colletotrichun pathogens of strawberry and design of PCR primers for their identification. Phytopathology, 2003, 151:135-143
[17]王晓鸣·六种重要玉米病害病原名称的厘定·中国农业科学,2018, 51(18):3497-3507Wang X M. Collation of scientific names of six maize disease pathogens. Scientia Agricultura Sinica, 2018, 51(18):3497-3507
[18] Novicki T J, Lafe K, Bui L, Bui U, Geise R, Marr K, Cookson B T-Genetic diversity among clinical isolates of acremonium strictum determined during an investigation of a fatal mycosis.Journal of Clinical Microbiology, 2003,41(6):2623-2628
[19] Summerbell R C, Gueidan C, Schroers H J, Dehoog G S,Starink M, Rosete Y A, Guarro J, Scott J A-Acremonium phylogenetic overview and revision of gliomastix, sarocladium,and trichothecium-Studies in Mycology, 2011,68:139-162