温阳通络方对去势雌性大鼠瘦素、脂联素及骨密度的影响
摘要
目的:观察温阳通络方对去势雌性大鼠骨密度及脂肪因子的影响,探讨温阳通络方治疗骨质疏松症的作用机制。方法:48只SPF级SD雌性大鼠分为4组,假手术组、模型组、中药组及西药对照组,模型组、中药组及西药对照组采用双侧卵巢并行氢化可的松皮下注射的方法造模,假手术组采用切除卵巢周边与卵巢等量的脂肪,中药组灌胃温阳通络方,西药组给与雌激素戊酸雌二醇片,假手术组、模型组灌服等量蒸馏水,12周后进行称重及骨密度检测,摘眼球采血,Elisa法检测血清瘦素及脂联素水平。结果:各组大鼠造模前及给药前体重无差异;经药物干预12周后各组大鼠体重均有增加,与假手术组比较,模型组、中药组及西药对照组大鼠体重均有显著升高(P<0.05);与模型组比较,中药温阳通络方及西药对照组体重显著降低(P<0.05),中药组与西药对照组两组体重差异无统计学意义(P>0.05);瘦素水平中,中药组与西药对照组比较,西药对照组水平稍高,但差异无统计学意义(P>0.05);在脂联素水平方面,模型组与假手术组比较差异有统计学意义(P<0. 05);经给药干预后,中药组、西药对照组与模型组比较差异有统计学意义(P<0. 05),中药组与西药对照组两组间差异无统计学意义(P>0.05)。结论:温阳通络方可有效增加去除卵巢联合氢化可的松造模成功的肾阳虚证大鼠的骨密度,并明显提高瘦素水平并降低脂联素水平,促进成骨、抑制破骨。
引文
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[3]陈剑磨,张胜军,夏炳江.骨质疏松肾阳虚病证结合模型构建的实验研究[J].中国中医急症,2015,24(2):201-203,254.
[4]Kershaw EE,Flier JS.Adipose tissue as an endocrine organ[J].JClin Endocrinol Metab,2004,89(6):2548-2556.
[5]Zhang Y,Proenca R,Maffei M,et al.Positional cloning of the mouse obese gene and its human homologue[J].Nature,1994,372(6505):425-432.
[6]宋守君.瘦素与衰老[J].老年医学与保健,2000,(6):44-45.
[7]李华青,刘师伟,刘伯轩,等.瘦素对人骨髓间充质干细胞成骨分化的影响[J].中国组织工程研究与临床康复,2009,13(23):4536-4540.
[8]熊成海,刘保龙,闫华,等.升高脑脊液瘦素水平预防兔骨质疏松的实验研究[J].中华骨科杂志,2016,36(18):1191-1201.
[9]Scherer PE,Williams S,Foglinano M,et al.A novel serum protein similar to C1q,produced exclusively in adipocytes[J].J Biol Chem,1995,270(45):26746-26749.
[10]Shinoda Y,Yamaguchi M,Ogata N,et al.Regulation of bone formation by adiponectin through autocrine/paracrine and endocrine pathways[J].J Cell Biochem,2006,99(1):196-208.
[11]Kontogianni MD,Dafni UG,Routsias JG,et al.Blood leptin and adiponectin as possible mediators of the relation between fat mass and BMD in perimenopausal women[J].J Bone Miner Res,2004,19(4):546-551.
[12]袁凌青,罗湘杭,谢辉,等.脂联素对人成骨细胞增殖作用的研究[J].中国骨质疏松杂志,2006,12(2):135-138.
[13]沈自尹.中医虚证辨证参考标准[J].中西医结合杂志,1986,6(10):598.
[14]Kim T,Ha H,Shim KS,et al.The anti-osteoporotic effect of Yijung-tang in an ovariectomized rat model mediated by inhibition of osteoclast differentiation[J].J Ethnopharmacol,2013,146(1):83-89.