黔产薏苡仁油成分分析及对破骨细胞活性的影响
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摘要
目的:分析黔产薏苡仁油成分并研究不同浓度黔产薏苡仁油对破骨细胞活性的影响。方法:采用无水乙醇超声辅助法提取黔产薏苡仁油,并对其进行脂肪酸残基的气相色谱-质谱联用(GC-MS)分析,以雌二醇(10-8mol/L)作为阳性对照,采用原代分离新西兰乳兔骨髓细胞,并用1α,25-(OH)_2Vit D_3诱导获得破骨细胞,通过抗酒石酸酸性磷酸酶(TRAP)+细胞数、TRAP酶活及骨陷窝面积,分析黔产薏苡仁油对破骨细胞活性的影响。结果:通过GC-MS分析黔产薏苡仁油的脂肪酸残基主要为十四烷酸(肉豆蔻酸)、十六烷酸(棕榈酸)、十八烷酸(硬脂酸)、十八碳一烯酸(油酸)、十八碳二烯酸(亚油酸)、二十二烷酸(山嵛酸)、角鲨烯和γ-谷甾醇;进一步实验表明,黔产薏苡仁油各浓度组(0.98、1.95、3.91、7.81、15.63、31.25μg/mL)对破骨细胞活性具有一定的抑制作用,其中以高浓度组31.25μg/mL对破骨细胞的活性的抑制作用最为显著(p <0.05),且呈现出较为良好的浓度依赖性。结论:黔产薏苡仁油对破骨细胞活性具有抑制作用。
Objective: To analyze the components of coix seed oil in Guizhou and study the effects of different concentrations of coix seed oil on osteoclast activity. Methods: The ethanol-assisted extraction of coix seed oil in Guizhou was carried out by anhydrous ethanol and the gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid residues was carried out.Estradiol (10-8 mol/L) was used as a positive control. Rabbit bone marrow cells were induced with 1α,25-(OH)_2 Vit D_3 to obtain osteoclasts.The effects of coix seed oil in Guizhou on osteoclast activity were analyzed by TRAP + cell number,TRAP activity and bone sag area. Results: The fatty acid residues of hazelnut oil produced by GC-MS were mainly myristic acid,palmitic acid,octadecanoic acid (stearic acid),and eighteen carbon monoenoic acid (oleic acid),octadecadienoic acid (linoleic acid),behenic acid,squalene and γ-sitosterol; further experiments showed that the concentration of coix seed oil in Guizhou (0.98,1.95,3.91,7.81,15.63,31.25 μg/mL) had a certain inhibitory effect on osteoclast activity,31.25 μg/mL in the high concentration group inhibited the activity of osteoclasts most significantly (p < 0.05),and showed a good concentration dependence.Conclusion: Coix seed oil in Guizhou has an inhibitory effect on osteoclast activity.
Objective: To analyze the components of coix seed oil in Guizhou and study the effects of different concentrations of coix seed oil on osteoclast activity. Methods: The ethanol-assisted extraction of coix seed oil in Guizhou was carried out by anhydrous ethanol and the gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid residues was carried out.Estradiol (10-8 mol/L) was used as a positive control. Rabbit bone marrow cells were induced with 1α,25-(OH)_2 Vit D_3 to obtain osteoclasts.The effects of coix seed oil in Guizhou on osteoclast activity were analyzed by TRAP + cell number,TRAP activity and bone sag area. Results: The fatty acid residues of hazelnut oil produced by GC-MS were mainly myristic acid,palmitic acid,octadecanoic acid (stearic acid),and eighteen carbon monoenoic acid (oleic acid),octadecadienoic acid (linoleic acid),behenic acid,squalene and γ-sitosterol; further experiments showed that the concentration of coix seed oil in Guizhou (0.98,1.95,3.91,7.81,15.63,31.25 μg/mL) had a certain inhibitory effect on osteoclast activity,31.25 μg/mL in the high concentration group inhibited the activity of osteoclasts most significantly (p < 0.05),and showed a good concentration dependence.Conclusion: Coix seed oil in Guizhou has an inhibitory effect on osteoclast activity.
引文
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[3]Gong X J,BAO YR,DIAO YP,et al,Adlay seed inhibits growth of H22 allografts,Hep G2 and SMMC-7721 human hepatoma cells in vitro and in vivo[J].Asian Journal of Chemistry,2012,24(2):128.
[4]Lin M H,Wu M C,Lu S,et al.Glycemic index,glycemic load and insulinemic index of Chinese starchy foods[J].World Journal of Gastroenterology,2010,16(39):4973-4979.
[5]Chung C P,Hsia S M,Lee M Y,et al.Gastroprotective activities of adlay(Coix lachryma-jobi L.var.ma-yuen Stapf)on the growth of the stomach cancer AGS cell line and indomethacin-induced gastric ulcers[J].J Agric Food Chem,2011,59(11):6025-6033.
[6]谭煌英,李园,于莉莉,等.康莱特对大鼠的镇痛作用及其对促炎细胞因子的影响[J].中国中西医结合外科杂志,2007,13(2):152-155.
[7]Fei Yu,Jing Gao,Yong Zeng,et al.Effects of adlay seed oil on blood lipids and antioxidant capacity in hyperlipidemic rats[J].Journal of the Science of Food and Agriculture,2011,91(10):1843-1848.
[8]Yang R S,Chiang W,Lu Y H,et al.Evaluation of osteoporosis prevention by adlay using a tissue culture model[J].Asia Pacific Journal of Clinical Nutrition,2008,17 Suppl 1(S1):143.
[9]田洪星,郑晓霞,胡蝶,等.薏苡仁的化学成分及质量控制研究进展[J].贵州农业科学,2017,45(7):82-87.
[10]Kogawa M,Anderson P H,Findlay D M,et al.The metabolism of 25-(OH)vitamin D3 by osteoclasts and their precursors regulates the differentiation of osteoclasts[J].Journal of Steroid Biochemistry&Molecular Biology,2010,121(1):277-280.
[11]黄俊飞,俸婷婷,陆毅,等.西瑞香素体外抑制破骨细胞分化和促进成骨细胞增殖的活性研究[J].中药药理与临床,2016(1):69-72.
[12]胡爱军,丘泰球,梁汉华.超声强化提取薏苡仁油的研究[J].现代化工,2002(10):34-37.
[13]Simonet W S,Lacey D L,Dunstan C R,et al.Osteoprotegerin:a novel secreted protein involved in the regulation of bone density[J].Cell,1997,89(2):309-319.
[14]王柄棋,罗文娟,孙雨晴,等.青娥方对去卵巢大鼠血清MMP-9、TRACP5b、Cath-K及骨组织形态结构的影响[J].福建中医药,2018,49(1):46-49.
[15]张春娥,张惠,刘楚怡,等.亚油酸的研究进展[J].粮油加工(电子版),2010(5):18-21.
[16]孔令杰,张东恒,魏观为,等.油酸组分对多元醇油酸酯抗氧化性影响的研究[J].润滑油,2015,30(6):38-41.
[17]刘纯友,马美湖,靳国锋,等.角鲨烯及其生物活性研究进展[J].中国食品学报,2015,15(5):147-156.
[18]马艳利,叶胜难.炎症激活的破骨细胞在胆脂瘤骨破坏中的作用[J].国际耳鼻咽喉头颈外科杂志,2006,30(3):164-166.