穴位注射疗法对脑瘫幼鼠脑组织及神经行为能力的影响
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摘要
目的:研究穴位注射疗法对脑瘫幼鼠脑组织神经细胞及肢体运动功能和学习记忆能力的影响并探讨相关机制。方法:采用颈动脉夹断法制备30只7日龄SD脑瘫幼鼠模型并随机分为3组:模型对照组、针灸组、穴位注射组,每组10只,另设10只7日龄SD健康幼鼠为正常对照组。穴位注射组分别于幼鼠左侧顶颞前斜线和右肢穴位处注射药物,针灸组在相同部位进行针刺。治疗结束后通过悬吊实验、斜坡实验、拒俘反应实验、Morris水迷宫实验等进行神经行为学的疗效评价。取各组幼鼠脑组织进行HE染色,计录正常神经细胞的数量,并检测海马区脑组织及血清中神经元特异性烯醇化酶(neuron-specific enolase,NSE)的含量。结果:穴位注射组各神经行为学指标均优于模型对照组及针灸组,差异有统计学意义(P <0. 05);与模型对照组和针灸组相比,穴位注射组脑组织中正常神经细胞数量较多,海马区NSE含量较高而血清中NSE水平降低。结论:穴位注射疗法可以有效提高脑瘫幼鼠的肢体运动和学习记忆能力,且效果优于单纯针灸疗法,其机制可能是在穴位与药物双重作用下,受损神经元得以修复,减少了NSE向血液中的渗漏,从而有效地改善脑瘫状态。
Objective: To study the effect of acupoint injection therapy on the motor function and learning and memory ability of cerebral nerve cells and limbs in infant rats with cerebral palsy and to explore the possible mechanism. Methods: 30 7-day-old SD cerebral palsy rats models were prepared by carotid artery clamping method and randomly divided into three groups: model control group,acupuncture group and acupoint injection group,10 in each group,and 10 healthy SD rats aged 7 days were set as normal control group. Acupoint injection group injected drugs at left parietal-temporal anterior oblique line and right limb acupoints respectively. Acupuncture group received acupuncture at the same location. After the treatment,the neurobehavioral effects were evaluated by suspension test,slope test,captive rejection test,morris water maze test and so on. HE staining was used to count the number of normal nerve cells in the brain tissues of young rats in each group,and the content of neuron-specific enolase( NSE) in hippocampus brain tissues and serum was detected. Results: The neurobehavioral indexes of acupoint injection group were better than those of model control group and acupuncture group. The difference was statistically significant( P < 0. 05). Compared with model control group and acupuncture group,the acupoint injection group had more normal neurons in brain tissue,higher NSE content in hippocampus and lower NSE level in serum. Conclusion: Acupoint injection therapy can effectively improve limb movement and learning and memory ability of cerebral palsy young rats,and its effect is better than that of acupuncture alone. The mechanism may be that under the dual action of acupoints and drugs,damaged neurons can be repaired,the leakage of NSE into blood is reduced,and the state of cerebral palsy can be effectively improved.
Objective: To study the effect of acupoint injection therapy on the motor function and learning and memory ability of cerebral nerve cells and limbs in infant rats with cerebral palsy and to explore the possible mechanism. Methods: 30 7-day-old SD cerebral palsy rats models were prepared by carotid artery clamping method and randomly divided into three groups: model control group,acupuncture group and acupoint injection group,10 in each group,and 10 healthy SD rats aged 7 days were set as normal control group. Acupoint injection group injected drugs at left parietal-temporal anterior oblique line and right limb acupoints respectively. Acupuncture group received acupuncture at the same location. After the treatment,the neurobehavioral effects were evaluated by suspension test,slope test,captive rejection test,morris water maze test and so on. HE staining was used to count the number of normal nerve cells in the brain tissues of young rats in each group,and the content of neuron-specific enolase( NSE) in hippocampus brain tissues and serum was detected. Results: The neurobehavioral indexes of acupoint injection group were better than those of model control group and acupuncture group. The difference was statistically significant( P < 0. 05). Compared with model control group and acupuncture group,the acupoint injection group had more normal neurons in brain tissue,higher NSE content in hippocampus and lower NSE level in serum. Conclusion: Acupoint injection therapy can effectively improve limb movement and learning and memory ability of cerebral palsy young rats,and its effect is better than that of acupuncture alone. The mechanism may be that under the dual action of acupoints and drugs,damaged neurons can be repaired,the leakage of NSE into blood is reduced,and the state of cerebral palsy can be effectively improved.
引文
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[11]吴胜兰.神经节苷脂与神经生长因子联用治疗小儿脑损伤临床观察[J].中国社区医师(医学专业),2011,25(7):98-99.
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[13]尤玉慧,崔志慧,尹水贵,等. 0-6个月龄脑瘫高危儿血清NSE表达特点及临床价值[J].医学研究杂志,2013,42(6):98-101.
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[15]李佩青,周伟,梁妤婷,等.血清神经元特异性烯醇化酶与新生儿缺氧缺血性脑病神经损伤的相关性研究[J].实用医学杂志,2013,29(17):2806-2808.
[16]李常新,黄如训,陈立云,等.电针对大脑中动脉闭塞大鼠脑内神经元特异性烯醇化酶及胶质纤维酸性蛋白表达的影响[J].中华物理医学与康复杂志,2003,25(12):708-712.
[17]BHAROSAY A,BHAROSAY V V,VARMA M,et al. Correlation of brain biomarker neuron specific enolase(NSE)with degree of disability and neurological worsening in cerebrovascular stroke[J]. Indian J Clinical Biochemistry,2012,27(2):186-190.
[18]胡晓丽,王雪峰,庞伟,等.输合配穴针刺对LPS脑损伤幼鼠模型脑组织S100B,NSE表达的影响[J].中国中西医结合儿科学,2015,7(1):20-22.
[19]刘青,曾志,周卓妍,等. EPO对新生大鼠缺氧缺血性脑损伤NSE表达的影响[J].暨南大学学报(自然科学与医学版),2011,32(6):593-597.
[2]GABIS,L V,TSUBARY N M. Assessment of Abilities and Comorbidities in Children With Cerebral Palsy[J]. J Child Neurol,2015,30(12):1640-1645.
[3]李恩耀,介小素,郭鑫,等.穴位注射对痉挛性脑瘫患儿肌张力及智力影响临床分析[J].中国实用神经疾病杂志,2010,13(7):63-64.
[4]胡晓丽,王雪峰,张亮,等.新生小鼠缺氧缺血性脑损伤模型的制作研究[J].现代生物医学进展,2014,28(1):13.
[5]朱士光,杨荣礼,耿德勤,等.局灶性脑缺血大鼠Z-Longa评分的MRI研究[J].国际老年医学杂志,2012,33(5):199-201.
[6]郭义,方剑乔.实验针灸学[M].北京:中国中医药出版社,2012.
[7]李恩耀,娄元俊,郭鑫,等.水针注射、针刺运动配合作业训练治疗小儿脑瘫精细运动功能障碍73例[J].中医杂志,2010,60(7):628-629.
[8]姚志勇.脑苷肌肽,维生素B1,维生素B12穴位注射治疗痉挛型脑性瘫痪效果观察[J].河南医学研究,2013,22(6):875-876.
[9]TYLICKI A,SIEMIENIUK M. Thiamine and its derivatives in the regulation of cell metabolism[J]. Postepy Hig Med Dosw(Online),2011,65:447-469
[10]李文梅.维生素B12对宫内发育迟缓大鼠脑损伤的干预研究[D].苏州:苏州大学,2014.
[11]吴胜兰.神经节苷脂与神经生长因子联用治疗小儿脑损伤临床观察[J].中国社区医师(医学专业),2011,25(7):98-99.
[12]李燕玲.神经节苷脂联合神经生长因子治疗小儿脑损伤的效果分析[J].现代中西医结合杂志,2012,21(14):1537-1537.
[13]尤玉慧,崔志慧,尹水贵,等. 0-6个月龄脑瘫高危儿血清NSE表达特点及临床价值[J].医学研究杂志,2013,42(6):98-101.
[14]魏风,陈俭.神经元特异性烯醇化酶在颅脑损伤中的研究进展[J].医学综述,2012,18(1):13-15.
[15]李佩青,周伟,梁妤婷,等.血清神经元特异性烯醇化酶与新生儿缺氧缺血性脑病神经损伤的相关性研究[J].实用医学杂志,2013,29(17):2806-2808.
[16]李常新,黄如训,陈立云,等.电针对大脑中动脉闭塞大鼠脑内神经元特异性烯醇化酶及胶质纤维酸性蛋白表达的影响[J].中华物理医学与康复杂志,2003,25(12):708-712.
[17]BHAROSAY A,BHAROSAY V V,VARMA M,et al. Correlation of brain biomarker neuron specific enolase(NSE)with degree of disability and neurological worsening in cerebrovascular stroke[J]. Indian J Clinical Biochemistry,2012,27(2):186-190.
[18]胡晓丽,王雪峰,庞伟,等.输合配穴针刺对LPS脑损伤幼鼠模型脑组织S100B,NSE表达的影响[J].中国中西医结合儿科学,2015,7(1):20-22.
[19]刘青,曾志,周卓妍,等. EPO对新生大鼠缺氧缺血性脑损伤NSE表达的影响[J].暨南大学学报(自然科学与医学版),2011,32(6):593-597.