河南不同地区棉蚜种群共生菌Wolbachia的检测及系统发育分析
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摘要
【目的】本研究旨在揭示河南省不同地区棉蚜Aphis gossypii种群共生菌Wolbachia的感染情况,明确Wolbachia的感染类型及分类地位。【方法】2016-2017年间采集河南省不同地区的13个棉蚜种群,通过扩增COI基因片段对其进行种类鉴定;通过扩增棉蚜种群中Wolbachia的wsp基因片段对其进行Wolbachia感染率的检测,应用neighbor-joining法构建系统进化树进行棉蚜种群中Wolbachia的系统发育分析。【结果】对河南省内不同地区采集的13个棉蚜种群的Wolbachia感染率而言,郑州(ZZ)种群最高(46.67%),信阳2(XY2)种群最低(6.67%),13个种群Wolbachia的感染率范围为6.67%～46.67%,平均感染率为28.35%。基于wsp基因构建的系统发育树表明,安阳和信阳的棉蚜种群感染的Wolbachia株系属于B大组,其余地区棉蚜种群感染的Wolbachia株系属于A大组。【结论】河南省不同地区的棉蚜种群Wolbachia感染率差别较大,且不同种群感染的Wolbachia株系分别属于A大组或B大组。
【Aim】 This study aims to investigate the infection of secondary endosymbiont Wolbachia in Aphis gossypii populations from different areas of Henan province, central China, and further to ascertain its infection type and phylogeny. 【Methods】 A. gossypii samples were collected from 13 areas of Henan province during 2016-2017, and identified by amplifying the COI gene. The infection rate of Wolbachia was detected by amplification of the wsp gene sequence from the Wolbachia in different A. gossypii populations. The phylogenetic relationship of Wolbachia in different A. gossypii populations was constructed with neighbor-joining method. 【Results】 The infection rates of Wolbachia in 13 populations of A. gossypii collected from different areas of Henan province were different, being the highest(46.67%) in Zhengzhou(ZZ) population and the lowest(6.67%) in Xinyang 2(XY2) population. The infection rates of Wolbachia in the 13 populations ranged from 6.67% to 46.67%, with the average value of 28.35%. The phylogenetic relationship based on wsp gene indicated that Wolbachia strains in both Anyang and Xinyang populations of A. gossypii were identified as supergroup B, while those in other populations were supergroup A. 【Conclusion】 The infection rate of Wolbachia in different A. gossypii populations of Henan province varies distinctly, and either supergroup A or B Wolbachia is present in these A. gossypii populations.
【Aim】 This study aims to investigate the infection of secondary endosymbiont Wolbachia in Aphis gossypii populations from different areas of Henan province, central China, and further to ascertain its infection type and phylogeny. 【Methods】 A. gossypii samples were collected from 13 areas of Henan province during 2016-2017, and identified by amplifying the COI gene. The infection rate of Wolbachia was detected by amplification of the wsp gene sequence from the Wolbachia in different A. gossypii populations. The phylogenetic relationship of Wolbachia in different A. gossypii populations was constructed with neighbor-joining method. 【Results】 The infection rates of Wolbachia in 13 populations of A. gossypii collected from different areas of Henan province were different, being the highest(46.67%) in Zhengzhou(ZZ) population and the lowest(6.67%) in Xinyang 2(XY2) population. The infection rates of Wolbachia in the 13 populations ranged from 6.67% to 46.67%, with the average value of 28.35%. The phylogenetic relationship based on wsp gene indicated that Wolbachia strains in both Anyang and Xinyang populations of A. gossypii were identified as supergroup B, while those in other populations were supergroup A. 【Conclusion】 The infection rate of Wolbachia in different A. gossypii populations of Henan province varies distinctly, and either supergroup A or B Wolbachia is present in these A. gossypii populations.
引文
Aldyhim YN, Khalil AF, 1993. Influence of temperature and day length on population development of Aphis gossypii on Cucurbita pepo. Entomol. Exp. Appl., 67(2): 167-172.
Augustinos AA, Santos-Garcia D, Dionyssopoulou E, Moreira M, Papapanagiotou A, Scarvelakis M, Doudoumis V, Ramos S, Aguiar AF, Borges PAV, Khadem M, Latorre A, Tsiamis G, Bourtzis K, 2011. Detection and characterization of Wolbachia infections in natural populations of aphids: is the hidden diversity fully unraveled? PLoS ONE, 6(12): e28695.
Baldo L, Dunning Hotopp JC, Jolley KA, Bordenstein SR, Biber SA, Choudhury RR, Hayashi C, Maiden MC, Tettelin H, Werren JH, 2006. Multilocus sequence typing system for the endosymbiont Wolbachia pipientis. Appl. Environ. Microbiol., 72(11): 7098-7110.
Bing XL, Rao Q, Luan JB, Liu SS, 2014. The distribution, transmission, functions and genomics of the symbiont Hamiltonella of invertebrates. J. Environ. Entomol., 36(2): 252-259. [邴孝利, 饶琼, 栾军波, 刘树生, 2014. 节肢动物共生细菌Hamiltonella的分布、传播、功能及基因组研究. 环境昆虫学报, 36(2): 252-259]
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotech., 3(5): 294-299.
Glaser RL, Meola MA, 2011. The native Wolbachia endosymbionts of Drosophila melanogaster and Culex quinquefasciatus increase host resistance to West Nile Virus infection. PLoS ONE, 5(8): e11977.
Gómez-Valero L, Soriano-Navarro M, Perez-Brocal V, Heddi A, Moya A, Garcia-Verdugo JM, Latorre A, 2004. Coexistence of Wolbachia with Buchnera aphidicola and a secondary symbiont in the aphid Cinara cedri. J. Bacteriol., 186(19): 6626-6633.
Guo W, Shen ZR, 2004. Molecular identification of wsp gene of Wolbachia infected in Aphis gossypii Glover. J. Microbiol., 24(2): 1-3. [国伟, 沈佐锐, 2004. 棉蚜体内感染沃尔巴克氏体(Wolbachia)的分子检测. 微生物学杂志, 24(2): 1-3]
Huigens ME, Luck RF, Klaassen RHG, Maas MFPM, Timmermans MJTN, Stouthamer R, 2000. Infectious parthenogenesis. Nature, 405(6783): 178-179.
Hurst GDD, Majerus MEN, Walker LE, 1993. The importance of cytoplasmic male killing elements in natural populations of the two spot ladybird, Adalia bipunctata (Linnaeus) (Coleoptera: Coccinellidae). Biol. J. Linn. Soc., 49(2): 195-202.
Jeyaprakash A, Hoy MA, 2000. Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species. Insect Mol. Biol., 9(4): 393-405.
Jones RT, Bressan A, Greenwell AM, Fierer N, 2011. Bacterial communities of two parthenogenetic aphid species cocolonizing two host plants across the Hawaiian Islands. Appl. Environ. Microb., 77(23): 8345-8349.
Kersting U, Sater S, Uygun N, 1999. Effect of temperature on development rate and fecundity of apterous Aphis gossypii Glover (Hom., Aphididae) reared on Gosspium hirsutum L. J. Appl. Entomol., 123(1): 23-27.
Kozek WJ, Rao RU, 2007. The discovery of Wolbachia in arthropods and nematodes-a historial perspective. In: Hoefauf A, Rao RU eds. Wolbachia: A Bug’s Life in Another Bug. Issues in Infectious Diseases, Vol. 5. Basel, Karger. 1-14.
Li T, Wu YQ, Xiao JH, Duan Y, Jiang YL, Miao J, Gong ZJ, 2013. Detection and phylogenetic analysis of Wolbachia in wheat and soybean aphids in China. Acta Entomol. Sin., 56(2): 195-200. [李彤, 武予清, 肖金花, 段云, 蒋月丽, 苗进, 巩中军, 2013. 小麦和大豆蚜虫中内共生菌Wolbachia的感染检测和系统发育分析. 昆虫学报, 56(2): 195-200]
Qu AJ, Wei ZS, Zhu CM, Wang ZW, Sun XG, Zhang WG, Chen ZL, 2004. Study on selectivity of Aphis gossypii to host plant odour sources. J. Shandong Agric. Univ. (Nat. Sci.), 35(3): 363-367. [曲爱军, 魏志顺, 朱承美, 王志武, 孙绪艮, 张卫光, 陈子雷, 2004. 棉蚜对寄主植物的选择性研究. 山东农业大学学报(自然科学版), 35(3): 363-367]
Rigaud T, Souty-Grosset C, Raimond R, Mocquard J, Juchault P, 1991. Feminizing endocytobiosis in the terrestrial crustacean Armadillidium vulgare Latr. (Isopoda): recent acquisitions. Endocyt. Cell Res., 7(3): 259-273.
Satar S, Kersting U, Uygun N, 1999. Development and fecundity of Aphis gossypii Glover (Homoptera: Aphididae) on three Malvaceae hosts. Turk. J. Agric. For., 23(6): 637-643.
Satar S, Kersting U, Uygun N, 2005. Effect of temperature on development and fecundity of Aphis gossypii Glover (Homoptera: Aphididae) on cucumber. J. Pest Sci., 78(3): 133-137.
Sepúlveda DA, Zepeda-Paulo F, Ramírez CC, Lavandero B, Figueroa CC, 2017. Diversity, frequency, and geographic distribution of facultative bacterial endosymbionts in introduced aphid pests. Insect Sci., 24(3): 511-521.
Sintupachee S, Milne JR, Poonchaisri S, Baimai V, Kittayapong P, 2006. Closely related Wolbachia strains within the pumpkin arthropod community and the potential for horizontal transmission via the plant. Microb. Ecol., 51(3): 294-301.
Song Y, Fan YL, Wu LJ, Zhang ZF, Liu YH, Liu TX, 2014. Comparison and optimization of genomic DNA extraction from endosymbionts of aphids. Acta Phytophy. Sin., 41(6): 643-648. [宋月, 樊永亮, 武丽娟, 张战凤, 刘艳红, 刘同先, 2014. 蚜虫内共生菌基因组DNA提取方法的比较和优化. 植物保护学报, 41(6): 643-648]
Stouthamer R, Breeuwert JA, Luck RF, Werren JH, 1993. Molecular identification of microorganisms associated with parthenogenesis. Nature, 361(6407): 66-68.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S, 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol., 30(12): 2725-2729.
Tsuchida T, Koga R, Shibao H, Matsumoto T, Fukatsu T, 2002. Diversity and geographic distribution of secondary endosymbiotic bacteria in natural populations of the pea aphid, Acyrthosiphon pisum. Mol. Ecol., 11(10): 2123-2135.
Wang Z, Shen ZR, Song Y, Liu HY, Li ZX, 2009. Distribution and diversity of Wolbachia in different populations of the wheat aphid Sitobion miscanthi (Hemiptera: Aphididae) in China. Eur. J. Entomol., 106(1): 49-55.
Wang Z, Su XM, Wen J, Jiang LY, Qiao GX, 2014. Widespread infection and diverse infection patterns of Wolbachia in Chinese aphids. Insect Sci., 21(3): 313-325.
Werren JH, Windsor D, Guo L, 1995. Distribution of Wolbachia among neotropical arthropods. Proc. R. Soc. B Biol. Sci., 262(1364): 197-204.
West SA, Cook JM, Werren JH, Godfray HC, 1998. Wolbachia in two insect host-parasitoid communities. Mol. Ecol., 7(11): 1457-1465.
Wu YX, Zhang CL, Hong XY, 2008. Analysis of the phylogenetic relationships between Myzus persicae and its endosymbiotic bacterium Buchnera. J. Nanjing Agric. Univ., 31(1): 51-56. [吴玉新, 张春玲, 洪晓月, 2008. 桃蚜与其内共生菌Buchnera之间的系统进化关系分析. 南京农业大学学报, 31(1): 51-56]
Xiao YL, Yin XC, Liu TX, 2013. Performance of the two host-biotypes of Aphis gossypii (Hemiptera: Aphididae) on different cucurbitaceous host plants. Acta Ecol. Sin., 33(12): 3706-3711. [肖云丽, 印象初, 刘同先, 2013. 不同生物型棉蚜对夏寄主葫芦科作物的选择. 生态学报, 33(12): 3706-3711]
Yen JH, Barr AR, 1971. New hypothesis of the cause of cytoplasmic incompatibility in Culex pipiens L. Nature, 232(5313): 657-658.
Zhou W, Rousset F, O′Neill S, 1998. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proc. R. Soc. B Biol. Sci., 265(1395): 509-515.
Augustinos AA, Santos-Garcia D, Dionyssopoulou E, Moreira M, Papapanagiotou A, Scarvelakis M, Doudoumis V, Ramos S, Aguiar AF, Borges PAV, Khadem M, Latorre A, Tsiamis G, Bourtzis K, 2011. Detection and characterization of Wolbachia infections in natural populations of aphids: is the hidden diversity fully unraveled? PLoS ONE, 6(12): e28695.
Baldo L, Dunning Hotopp JC, Jolley KA, Bordenstein SR, Biber SA, Choudhury RR, Hayashi C, Maiden MC, Tettelin H, Werren JH, 2006. Multilocus sequence typing system for the endosymbiont Wolbachia pipientis. Appl. Environ. Microbiol., 72(11): 7098-7110.
Bing XL, Rao Q, Luan JB, Liu SS, 2014. The distribution, transmission, functions and genomics of the symbiont Hamiltonella of invertebrates. J. Environ. Entomol., 36(2): 252-259. [邴孝利, 饶琼, 栾军波, 刘树生, 2014. 节肢动物共生细菌Hamiltonella的分布、传播、功能及基因组研究. 环境昆虫学报, 36(2): 252-259]
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotech., 3(5): 294-299.
Glaser RL, Meola MA, 2011. The native Wolbachia endosymbionts of Drosophila melanogaster and Culex quinquefasciatus increase host resistance to West Nile Virus infection. PLoS ONE, 5(8): e11977.
Gómez-Valero L, Soriano-Navarro M, Perez-Brocal V, Heddi A, Moya A, Garcia-Verdugo JM, Latorre A, 2004. Coexistence of Wolbachia with Buchnera aphidicola and a secondary symbiont in the aphid Cinara cedri. J. Bacteriol., 186(19): 6626-6633.
Guo W, Shen ZR, 2004. Molecular identification of wsp gene of Wolbachia infected in Aphis gossypii Glover. J. Microbiol., 24(2): 1-3. [国伟, 沈佐锐, 2004. 棉蚜体内感染沃尔巴克氏体(Wolbachia)的分子检测. 微生物学杂志, 24(2): 1-3]
Huigens ME, Luck RF, Klaassen RHG, Maas MFPM, Timmermans MJTN, Stouthamer R, 2000. Infectious parthenogenesis. Nature, 405(6783): 178-179.
Hurst GDD, Majerus MEN, Walker LE, 1993. The importance of cytoplasmic male killing elements in natural populations of the two spot ladybird, Adalia bipunctata (Linnaeus) (Coleoptera: Coccinellidae). Biol. J. Linn. Soc., 49(2): 195-202.
Jeyaprakash A, Hoy MA, 2000. Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species. Insect Mol. Biol., 9(4): 393-405.
Jones RT, Bressan A, Greenwell AM, Fierer N, 2011. Bacterial communities of two parthenogenetic aphid species cocolonizing two host plants across the Hawaiian Islands. Appl. Environ. Microb., 77(23): 8345-8349.
Kersting U, Sater S, Uygun N, 1999. Effect of temperature on development rate and fecundity of apterous Aphis gossypii Glover (Hom., Aphididae) reared on Gosspium hirsutum L. J. Appl. Entomol., 123(1): 23-27.
Kozek WJ, Rao RU, 2007. The discovery of Wolbachia in arthropods and nematodes-a historial perspective. In: Hoefauf A, Rao RU eds. Wolbachia: A Bug’s Life in Another Bug. Issues in Infectious Diseases, Vol. 5. Basel, Karger. 1-14.
Li T, Wu YQ, Xiao JH, Duan Y, Jiang YL, Miao J, Gong ZJ, 2013. Detection and phylogenetic analysis of Wolbachia in wheat and soybean aphids in China. Acta Entomol. Sin., 56(2): 195-200. [李彤, 武予清, 肖金花, 段云, 蒋月丽, 苗进, 巩中军, 2013. 小麦和大豆蚜虫中内共生菌Wolbachia的感染检测和系统发育分析. 昆虫学报, 56(2): 195-200]
Qu AJ, Wei ZS, Zhu CM, Wang ZW, Sun XG, Zhang WG, Chen ZL, 2004. Study on selectivity of Aphis gossypii to host plant odour sources. J. Shandong Agric. Univ. (Nat. Sci.), 35(3): 363-367. [曲爱军, 魏志顺, 朱承美, 王志武, 孙绪艮, 张卫光, 陈子雷, 2004. 棉蚜对寄主植物的选择性研究. 山东农业大学学报(自然科学版), 35(3): 363-367]
Rigaud T, Souty-Grosset C, Raimond R, Mocquard J, Juchault P, 1991. Feminizing endocytobiosis in the terrestrial crustacean Armadillidium vulgare Latr. (Isopoda): recent acquisitions. Endocyt. Cell Res., 7(3): 259-273.
Satar S, Kersting U, Uygun N, 1999. Development and fecundity of Aphis gossypii Glover (Homoptera: Aphididae) on three Malvaceae hosts. Turk. J. Agric. For., 23(6): 637-643.
Satar S, Kersting U, Uygun N, 2005. Effect of temperature on development and fecundity of Aphis gossypii Glover (Homoptera: Aphididae) on cucumber. J. Pest Sci., 78(3): 133-137.
Sepúlveda DA, Zepeda-Paulo F, Ramírez CC, Lavandero B, Figueroa CC, 2017. Diversity, frequency, and geographic distribution of facultative bacterial endosymbionts in introduced aphid pests. Insect Sci., 24(3): 511-521.
Sintupachee S, Milne JR, Poonchaisri S, Baimai V, Kittayapong P, 2006. Closely related Wolbachia strains within the pumpkin arthropod community and the potential for horizontal transmission via the plant. Microb. Ecol., 51(3): 294-301.
Song Y, Fan YL, Wu LJ, Zhang ZF, Liu YH, Liu TX, 2014. Comparison and optimization of genomic DNA extraction from endosymbionts of aphids. Acta Phytophy. Sin., 41(6): 643-648. [宋月, 樊永亮, 武丽娟, 张战凤, 刘艳红, 刘同先, 2014. 蚜虫内共生菌基因组DNA提取方法的比较和优化. 植物保护学报, 41(6): 643-648]
Stouthamer R, Breeuwert JA, Luck RF, Werren JH, 1993. Molecular identification of microorganisms associated with parthenogenesis. Nature, 361(6407): 66-68.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S, 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol., 30(12): 2725-2729.
Tsuchida T, Koga R, Shibao H, Matsumoto T, Fukatsu T, 2002. Diversity and geographic distribution of secondary endosymbiotic bacteria in natural populations of the pea aphid, Acyrthosiphon pisum. Mol. Ecol., 11(10): 2123-2135.
Wang Z, Shen ZR, Song Y, Liu HY, Li ZX, 2009. Distribution and diversity of Wolbachia in different populations of the wheat aphid Sitobion miscanthi (Hemiptera: Aphididae) in China. Eur. J. Entomol., 106(1): 49-55.
Wang Z, Su XM, Wen J, Jiang LY, Qiao GX, 2014. Widespread infection and diverse infection patterns of Wolbachia in Chinese aphids. Insect Sci., 21(3): 313-325.
Werren JH, Windsor D, Guo L, 1995. Distribution of Wolbachia among neotropical arthropods. Proc. R. Soc. B Biol. Sci., 262(1364): 197-204.
West SA, Cook JM, Werren JH, Godfray HC, 1998. Wolbachia in two insect host-parasitoid communities. Mol. Ecol., 7(11): 1457-1465.
Wu YX, Zhang CL, Hong XY, 2008. Analysis of the phylogenetic relationships between Myzus persicae and its endosymbiotic bacterium Buchnera. J. Nanjing Agric. Univ., 31(1): 51-56. [吴玉新, 张春玲, 洪晓月, 2008. 桃蚜与其内共生菌Buchnera之间的系统进化关系分析. 南京农业大学学报, 31(1): 51-56]
Xiao YL, Yin XC, Liu TX, 2013. Performance of the two host-biotypes of Aphis gossypii (Hemiptera: Aphididae) on different cucurbitaceous host plants. Acta Ecol. Sin., 33(12): 3706-3711. [肖云丽, 印象初, 刘同先, 2013. 不同生物型棉蚜对夏寄主葫芦科作物的选择. 生态学报, 33(12): 3706-3711]
Yen JH, Barr AR, 1971. New hypothesis of the cause of cytoplasmic incompatibility in Culex pipiens L. Nature, 232(5313): 657-658.
Zhou W, Rousset F, O′Neill S, 1998. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proc. R. Soc. B Biol. Sci., 265(1395): 509-515.