紫外线等离子体复合诱变赤红球菌提高红色素产量
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摘要
为提高赤红球菌产红色素的能力,以实验室保藏的赤红球菌(Rhodococcus ruber YM-2)为出发菌株,采用等离子体单独诱变、等离子体连续诱变、紫外线单独诱变、紫外线等离子体复合诱变等方法,最终在等离子体连续诱变处理分别45、30 s时,经筛选得到一株遗传稳定的突变株A_2,红色素粗品产量达到1.12 g/L,相比于出发菌株提高了30.32%。同时发现等离子连续诱变比等离子体、紫外线单独诱变,或紫外线和等离子复合诱变的正突变率高,达到39.40%,等离子体连续诱变可作为一种高效的诱变手段。
In order to improve the ability of Rhodococcus ruber to produce red pigment,an original strain Rhodococcus ruber YM-2 preserved by our laboratory was used to be screened with plasma mutation,plasmaplasma continuous mutation,ultraviolet mutation,ultraviolet-plasma compound mutation. Under the condition of plasma-plasma continuous treatment at 45,30 s,a genetically stable mutant A_2 was screened by screening plate and flask fermentation culture. The red pigment yield of A_2 was 1.12 g/L,increased by 30.32 % compared with that of the original strain YM-2. In addition,comparing the effect of plasma,ultraviolet and ultravioletplasma treatments on positive mutation rate,the positive mutation rate of plasma-plasma continuous mutation was the highest 39.40 %,plasma-plasma continuous mutation could be used as a highly effective mutation method.
In order to improve the ability of Rhodococcus ruber to produce red pigment,an original strain Rhodococcus ruber YM-2 preserved by our laboratory was used to be screened with plasma mutation,plasmaplasma continuous mutation,ultraviolet mutation,ultraviolet-plasma compound mutation. Under the condition of plasma-plasma continuous treatment at 45,30 s,a genetically stable mutant A_2 was screened by screening plate and flask fermentation culture. The red pigment yield of A_2 was 1.12 g/L,increased by 30.32 % compared with that of the original strain YM-2. In addition,comparing the effect of plasma,ultraviolet and ultravioletplasma treatments on positive mutation rate,the positive mutation rate of plasma-plasma continuous mutation was the highest 39.40 %,plasma-plasma continuous mutation could be used as a highly effective mutation method.
引文
[1]车双辉,杜琪珍,夏明.天然蓝色素的研究进展[J].食品研究与开发,2003,24(2):18-20
[2]于淼,侯莉,黄娟,等.产红色素赤红球菌的筛选鉴定和产色素条件的优化[J].食品科技,2017,42(2):2-8
[3]刘小娟.产红色素菌株的筛选、鉴定、色素提取、红色素发酵工艺及性质的研究[D].安徽:安徽农业大学,2008
[4]赵海军,汪朝阳,侯晓娜.天然红色素的提取研究进展[J].广州化学,2008(3):32-39
[5]石永峰.国外食品天然色素的研究进展[J].粮食与食品工业,1996(4):23-27
[6]张雪,张晓菲,王立言,等.常压室温等离子体生物诱变育种及其应用研究进展[J].化工学报,2014,65(7):2676-2684
[7]刘燕,杨建松,王露,等.等离子诱变选育柠檬酸高产菌[J].天津科技大学学报,2014,29(1):16-19,35
[8]程功,徐建中,郭燕风,等.常压室温等离子体诱变选育L-精氨酸生产菌及发酵条件优化[J].微生物学通报,2016,43(2):360-369
[9]朱传合,贺亚男,路福平,等.N+离子注入阿维拉霉素产生菌诱变效应的研究[J].核技术,2006,29(8):609-613
[10]石秀峰,纪曾春,常传友,等.利用多功能等离子体诱变系统快速诱变选育γ-氨基丁酸高产菌株[J].中国酿造,2016,35(4):127-130
[11]黎恒,张琳,陈红爽,等.紫外线等离子体复合诱变解淀粉芽孢杆菌提高NAG产量[J].食品研究与开发,2018,39(12):172-176
[12]Ueno R,Hamada-Sato N,Ishida M,et al.Potential of carotenoids in aquatic yeasts as a phylogenetic reliable marker and natural colorant for aquaculture[J].Biosci Biotechnol Biochem,2011,75(9):1654-1661
[13]吴鹤云.常压室温等离子诱变结合高通量筛选选育尿苷产生菌[D].天津:天津科技大学,2016
[14]Morfill G E,Kong M G,Zimmermann J L.Focus on Plasma Medicine[J].New Journal of Physics,2009,11(11):115011
[15]蒋汶,张庆庆,汤文晶,等.紫外-等离子体复合诱变红曲霉产胞外多糖[J].食品与发酵工业,2016,42(1):64-69
[2]于淼,侯莉,黄娟,等.产红色素赤红球菌的筛选鉴定和产色素条件的优化[J].食品科技,2017,42(2):2-8
[3]刘小娟.产红色素菌株的筛选、鉴定、色素提取、红色素发酵工艺及性质的研究[D].安徽:安徽农业大学,2008
[4]赵海军,汪朝阳,侯晓娜.天然红色素的提取研究进展[J].广州化学,2008(3):32-39
[5]石永峰.国外食品天然色素的研究进展[J].粮食与食品工业,1996(4):23-27
[6]张雪,张晓菲,王立言,等.常压室温等离子体生物诱变育种及其应用研究进展[J].化工学报,2014,65(7):2676-2684
[7]刘燕,杨建松,王露,等.等离子诱变选育柠檬酸高产菌[J].天津科技大学学报,2014,29(1):16-19,35
[8]程功,徐建中,郭燕风,等.常压室温等离子体诱变选育L-精氨酸生产菌及发酵条件优化[J].微生物学通报,2016,43(2):360-369
[9]朱传合,贺亚男,路福平,等.N+离子注入阿维拉霉素产生菌诱变效应的研究[J].核技术,2006,29(8):609-613
[10]石秀峰,纪曾春,常传友,等.利用多功能等离子体诱变系统快速诱变选育γ-氨基丁酸高产菌株[J].中国酿造,2016,35(4):127-130
[11]黎恒,张琳,陈红爽,等.紫外线等离子体复合诱变解淀粉芽孢杆菌提高NAG产量[J].食品研究与开发,2018,39(12):172-176
[12]Ueno R,Hamada-Sato N,Ishida M,et al.Potential of carotenoids in aquatic yeasts as a phylogenetic reliable marker and natural colorant for aquaculture[J].Biosci Biotechnol Biochem,2011,75(9):1654-1661
[13]吴鹤云.常压室温等离子诱变结合高通量筛选选育尿苷产生菌[D].天津:天津科技大学,2016
[14]Morfill G E,Kong M G,Zimmermann J L.Focus on Plasma Medicine[J].New Journal of Physics,2009,11(11):115011
[15]蒋汶,张庆庆,汤文晶,等.紫外-等离子体复合诱变红曲霉产胞外多糖[J].食品与发酵工业,2016,42(1):64-69