低温快速成型多孔PLGA/COLⅡ支架的细胞生物相容性研究
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摘要
目的:评估多孔聚乳酸–羟基乙酸共聚物(PLGA)/Ⅱ型胶原(COLⅡ)软骨组织工程支架复合物的生物相容性。方法:通过低温快速成型技术(LDM)制备多孔PLGA支架,行低温等离子处理后复合COLⅡ,评估其力学性能。将多孔PLGA/COLⅡ软骨组织工程支架复合物与兔软骨细胞共培养,通过四甲基偶氮蓝比色法(MTT)测定检查细胞增殖,并通过扫描电子显微镜观察细胞与复合支架的黏附。结果:MTT检测实验组多孔复合支架细胞增殖与对照组比较,差异具有统计学意义(P <0.05);扫描电子显微镜显示兔软骨细胞在复合支架的表层和多孔结构中黏附、增殖和生长。结论:多孔PLGA/COLⅡ软骨组织工程复合支架可以适应软骨细胞的黏附和生长,具有良好的细胞相容性。
Objective To evaluate the biocompatibility of porous PLGA/COL Ⅱ composite cartilage tissue engineering scaffold. Method Porous PLGA scaffolds were prepared by low–temperature deposition manufacturing(LDM), and type Ⅱ collagen was grafted on the surface of porous PLGA by low temperature plasma treatment. Evaluate the mechanical properties of composite scaffold. The porous PLGA/COL Ⅱ cartilage tissue engineering composite scaffold was cocultured with rabbit chondrocytes, and cell proliferation was examined by MTT assay, and the adhesion of the cells to the composite scaffold was observed by scanning electron microscope(SEM). Results MTT assay showed that the proliferation of porous composite scaffold co–cultured cells in the experimental group was signi? cantly different from that in the control group(P < 0.05). SEM showed adhesion,proliferation and growth of rabbit chondrocytes on the surface and inside porous structure of the composite scaffold. Conclusion The porous PLGA/COL Ⅱ cartilage tissue engineering composite scaffold can adapt to the adhesion and growth of chondrocytes and has good biocompatibility.
Objective To evaluate the biocompatibility of porous PLGA/COL Ⅱ composite cartilage tissue engineering scaffold. Method Porous PLGA scaffolds were prepared by low–temperature deposition manufacturing(LDM), and type Ⅱ collagen was grafted on the surface of porous PLGA by low temperature plasma treatment. Evaluate the mechanical properties of composite scaffold. The porous PLGA/COL Ⅱ cartilage tissue engineering composite scaffold was cocultured with rabbit chondrocytes, and cell proliferation was examined by MTT assay, and the adhesion of the cells to the composite scaffold was observed by scanning electron microscope(SEM). Results MTT assay showed that the proliferation of porous composite scaffold co–cultured cells in the experimental group was signi? cantly different from that in the control group(P < 0.05). SEM showed adhesion,proliferation and growth of rabbit chondrocytes on the surface and inside porous structure of the composite scaffold. Conclusion The porous PLGA/COL Ⅱ cartilage tissue engineering composite scaffold can adapt to the adhesion and growth of chondrocytes and has good biocompatibility.
引文
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[2]Xie F,Kovic B,Jin X,et al.Economic and Humanistic Burden of Osteoarthritis:A Systematic Review of Large Sample Studies[J].Pharmacoeconomics,2016,34(11):1087-1100.
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[4]Liu W,Wang D,Huang J,et al.Low-temperature deposition manufacturing:A novel and promising rapid prototyping technology for the fabrication of tissue-engineered scaffold[J].Mater Sci Eng C Mater Biol Appl,2017,70(2):976-982.
[5]周勇,贾兆锋,刘威,等.聚乙烯醇/壳聚糖多孔水凝胶复合骨髓间充质干细胞修复膝关节软骨缺损[J].中国组织工程研究,2017,21(18):2881-2889.
[6]梁仟,朱飞燕,黄江鸿,等.Ⅱ型胶原水凝胶在软骨损伤修复中的研究进展[J].组织工程与重建外科杂志,2017,13(4):228-231.
[7]Huang J,Liu W,Liang Y,et al.Preparation and biocompatibility of diphasic magnetic nanocomposite scaffold[J].Mater Sci Eng C Mater Biol Appl,2018,87(6):70-77.
[8]黄江鸿,熊建义,王大平,等.低温快速成型3D打印磁性纳米复合人工骨的性能测定[J].海南医学,2017,28(4):525-530.
[9]Chang NJ,Lin CC,Shie MY,et al.Positive effects of cell-free porous PLGA implants and early loading exercise on hyaline cartilage regeneration in rabbits[J].Acta Biomater,2015,28(2):128-137.
[10]黄彰,王双利,潘政军,等.软骨PLGA-Ⅱ型胶原支架复合体修复兔膝关节骨软骨损伤[J].临床骨科杂志,2016,19(1):101-106.
[11]玄光善,穆兰兰,孙桐,等.等离子体处理聚氨酯-胶原-硫酸软骨素复合材料的制备及性能评价[J].中国组织工程研究,2016,20(43):6451-6457.
[12]Castillo-DalíG,Castillo-Oyagüe R,Terriza A,et al.Preprosthetic use of poly(lactic-co-glycolic acid)membranes treated with oxygen plasma and TiO2nanocomposite particles for guided bone regeneration processes[J].J Dent,2016,47(2):71-79.
[13]He Y,Liu W,Guan L,et al.A 3D-Printed PLCL Scaffold Coated with Collagen Type I and Its Biocompatibility[J].BioMed research international,2018,2018(12):5147156.
[14]欧阳建安,王大平,熊建义,等.新型钽铌多孔支架材料的细胞生物相容性研究[J].中国矫形外科杂志,2012,20(13):1226-1229.
[15]蒋婷,杨泽龙,李小兵.软骨脱细胞基质-Ⅱ型胶原纳米支架复合BMSC修复兔关节软骨缺损的实验研究[J].组织工程与重建外科杂志,2016,12(5):281-284.