推拿手法对坐骨神经损伤大鼠腓肠肌结蛋白表达的影响
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摘要
目的:研究推拿手法对延缓坐骨神经损伤大鼠腓肠肌萎缩的影响。方法:采用坐骨神经横断后外膜修复法建立坐骨神经损伤模型,将96只雄性SD大鼠按照体质量随机分为正常组、模型对照组、推拿手法组,每组分别于造模成功后第4、8、12、16周各取1小组8只大鼠检测取材,通过腓肠肌湿重恢复率评价肌肉萎缩情况,采用免疫组织化学方法观察腓肠肌内结蛋白(Desmin)表达情况。结果:推拿手法组和模型对照组的腓肠肌湿重恢复率均呈上升趋势,造模成功后第4、8周推拿手法组低于模型对照组(P>0.05),而第12、16周高于模型对照组(P<0.05);2组Desmin表达出现先降后升再降的变化趋势,造模成功后第4、8、12周推拿手法组高于模型对照组(P<0.05),第16周时低于模型对照组(P<0.05)。结论:推拿手法能够延缓坐骨神经损伤修复后骨骼肌萎缩,其作用机制可能通过上调Desmin表达促进新生肌细胞分化成熟实现。
Objective: To study the effect of Tuina manipulation on the gastrocnemius atrophy in rats with sciatic nerve injury.Methods: The model of sciatic nerve injury was established by repair of the outer membrane after the sciatic nerve transaction. A total of 96 male Sprague-Dawley(SD) rats were randomly divided into normal group, model group and Tuina group. The sciatic nerve was transected and the epineurium was sutured in the latter two groups. The Tuina group was given Tuina manipulation treatment once a day. Eight rats from each group were detected recovery rate of muscle wet weight to evaluate the recovery of muscular atrophy and Desmin expression to explore the mechanism of Tuina manipulation. Results: The recovery rate of muscle wet weight in the model group and the Tuina group were all ascended. Compared with the model group, the rate of the Tuina group was lower at the 4 and 8 weeks after successful modeling(P>0.05), higher at the 12 and 16 weeks after successful modeling(P<0.05). The expression of Desmin in the model group and the Tuina group showed a tendency to decrease at first, then increase and followed by decrease. The expression of Desmin in the Tuina group was higher at the 4, 8, 12 weeks after successful modeling(P<0.05), lower at the 16 weeks after successful modeling(P<0.05). Conclusion: Tuina can promote the recovery of gastrocnemius atrophy after sciatic nerve injury,and its mechanism may promote the differentiation and maturation of muscle satellite cells by up-regulating the expression of Desmin.
Objective: To study the effect of Tuina manipulation on the gastrocnemius atrophy in rats with sciatic nerve injury.Methods: The model of sciatic nerve injury was established by repair of the outer membrane after the sciatic nerve transaction. A total of 96 male Sprague-Dawley(SD) rats were randomly divided into normal group, model group and Tuina group. The sciatic nerve was transected and the epineurium was sutured in the latter two groups. The Tuina group was given Tuina manipulation treatment once a day. Eight rats from each group were detected recovery rate of muscle wet weight to evaluate the recovery of muscular atrophy and Desmin expression to explore the mechanism of Tuina manipulation. Results: The recovery rate of muscle wet weight in the model group and the Tuina group were all ascended. Compared with the model group, the rate of the Tuina group was lower at the 4 and 8 weeks after successful modeling(P>0.05), higher at the 12 and 16 weeks after successful modeling(P<0.05). The expression of Desmin in the model group and the Tuina group showed a tendency to decrease at first, then increase and followed by decrease. The expression of Desmin in the Tuina group was higher at the 4, 8, 12 weeks after successful modeling(P<0.05), lower at the 16 weeks after successful modeling(P<0.05). Conclusion: Tuina can promote the recovery of gastrocnemius atrophy after sciatic nerve injury,and its mechanism may promote the differentiation and maturation of muscle satellite cells by up-regulating the expression of Desmin.
引文
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[11]马书杰,严隽陶,黄品贤.推拿手法防治失神经骨骼肌萎缩的实验研究[J].江苏中医药,2014,46(11):79-81.
[12]马书杰,严隽陶,陶然,等.推拿手法联合跑台训练促进大鼠坐骨神经再生的效果[J].中国康复理论与实践,2016,22(11):1276-1280.
[13]陆永嘉,严隽陶,马书杰,等.推拿手法联合跑台训练对坐骨神经延期缝合大鼠神经再生的效果[J].中国康复理论与实践,2017,23(11):1273-1277.
[2]LUNDBORG G. A 25-year perspective of peripheral nerve surgery:evolving neuroscientific concepts and clinical significance[J].J Hand Surg Am,2000,25(3):391-414.
[3]PALLAFACCHINA G,FRANCOIS S,REGNAULT B,et al. An adult tissue-specific stem cell in its niche:a gene profiling analysis of in vivo quiescent and activated muscle satellite cells[J].Stem Cell Res,2010,4(2):77-91.
[4]马书杰,严隽陶.手法对家兔不同急性损伤骨骼肌卫星细胞影响观察[J].中华中医药学刊,2014,32(9):2118-2121.
[5]LOVERING R M,O`NEILL A,MURIEL J M,et al. Physiology structure and susceptibility to injury of skeketal muscle in mice lacking keratin 19-based and desmin-based intermediate filaments[J]. Am J Physiol Cell Physiol,2011,300(4):C803-C813.
[6]CHARRIER E E,ASNACIOS A,MILLOUD R,et al. Desmin mutation in the C-terminal domain impairs traction force generation in myoblasts[J]. Biophys J,2016,110(2):470-480.
[7]陈玉佩.电针“委中”穴对BPVC所致大鼠腰多裂肌损伤后修复作用和m TOR、Desmin表达的影响[D].北京:北京中医药大学,2016:1-5.
[8]LAWSON-SMITH M J,MCGEACHIE J K. The identification of myogenic cells in skeletal muscle,with emphasis on the use of tritiated thymidine autoradiography and desmin antibodies[J].J Anat,1998,192(Pt 2):161-171.
[9]高春刚,田野.补充谷氨酰胺对运动大鼠骨骼肌desmin表达的影响[J].中国运动医学杂志,2005,24(1):34-38.
[10]马书杰,严隽陶,陶然,等.手法联合跑台训练延缓周围神经损伤后骨骼肌萎缩的实验研究[J].康复学报,2016,26(5):29-32.
[11]马书杰,严隽陶,黄品贤.推拿手法防治失神经骨骼肌萎缩的实验研究[J].江苏中医药,2014,46(11):79-81.
[12]马书杰,严隽陶,陶然,等.推拿手法联合跑台训练促进大鼠坐骨神经再生的效果[J].中国康复理论与实践,2016,22(11):1276-1280.
[13]陆永嘉,严隽陶,马书杰,等.推拿手法联合跑台训练对坐骨神经延期缝合大鼠神经再生的效果[J].中国康复理论与实践,2017,23(11):1273-1277.