两种油脂对小鼠皮肤创伤修复作用研究
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摘要
本研究旨在观察阿勒泰大尾羊尾脂油脂对损伤皮肤是否具有修复作用,并与牛油脂、市售商品做比较。研究选择40只体重在31±1 g之间的昆明小鼠,雌雄各半,通过构建小鼠皮肤损伤模型,将小鼠分为4组(羊尾油组、牛油组、市售商品组和空白组)进行试验观察。小鼠皮肤伤口愈合良好,雌鼠的创面愈合能力强于雄鼠,雌鼠伤口愈合速度:羊尾油组>牛油组>市售商品组>空白对照组。雄鼠伤口愈合速度:羊尾油组>市售商品组>牛油组>空白对照组。皮肤组织病理学结果显示,涂抹羊尾脂、牛油脂、市售商品与空白对照处皮肤相比,创伤修复组织中脂肪细胞、胶原纤维以及其他细胞的数量、排列分布均有差异。羊尾油和牛油均有修复皮肤创伤的作用,且羊尾油修复作用更强,羊尾油可通过促进胶原纤维的生成加速皮肤修复。
The study aims at exploring the role of the tail lipids of Altay sheep in wound healing of skin, and comparing its healing effect with that of the cattle lipids and commercial products. Forty Kunming mice weighing 31 ± 1 g, half male and half female were selected. The wound models of mice skin were constructed,and then these mice were divided into four groups(sheep lipids group, cattle lipids group, commercial product group and blank control group). The wounds healed well after different treatments, the wound healing ability of female mice were better than that of the males, and the wound healing rates of female mice were: sheep lipids >cattle lipids > commercial product > blank control group, whereas those of the male mice were: sheep lipids >commercial product > cattle lipids > blank control group. The results of skin histopathology showed that compared with the blank control group, in the other three groups, the quantities and distribution of adipose cell, collagen fibrils and other cells in wound repair tissue were different. Both the sheep tail lipids and the cattle lipids have effects on repairing skin injury, and the sheep tail lipids have the better one. The sheep tail lipids can accelerate the skin repair by promoting collagen fibers producing.
The study aims at exploring the role of the tail lipids of Altay sheep in wound healing of skin, and comparing its healing effect with that of the cattle lipids and commercial products. Forty Kunming mice weighing 31 ± 1 g, half male and half female were selected. The wound models of mice skin were constructed,and then these mice were divided into four groups(sheep lipids group, cattle lipids group, commercial product group and blank control group). The wounds healed well after different treatments, the wound healing ability of female mice were better than that of the males, and the wound healing rates of female mice were: sheep lipids >cattle lipids > commercial product > blank control group, whereas those of the male mice were: sheep lipids >commercial product > cattle lipids > blank control group. The results of skin histopathology showed that compared with the blank control group, in the other three groups, the quantities and distribution of adipose cell, collagen fibrils and other cells in wound repair tissue were different. Both the sheep tail lipids and the cattle lipids have effects on repairing skin injury, and the sheep tail lipids have the better one. The sheep tail lipids can accelerate the skin repair by promoting collagen fibers producing.
引文
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[20]杨瑞,李亚洁.创伤修复动物模型的建立[J].护理研究,2003(23):1369-1370.
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[22]张彩丽,张俊霞,李瑞琴.小鼠皮肤组织石蜡切片制作经验[J].中国现代医药杂志,2016(5):91-92.
[23]尹玉鑫,施振声,曾申明.犬脂肪干细胞在犬皮肤创伤修复中的作用[J].犬业科技,2016(4):18-22.
[24]魏振东,郝泗城,孙建华,等.中药去腐生肌散等促进动物实验性皮肤溃疡修复作用的病理观察[J].天津医药,1990(09):547-548,577.
[25]郝薇,李福海,王玉林. Omega-3多不饱和脂肪酸对角化细胞及成纤维细胞的作用[J].山东大学学报,2012,50(9):50-54.
[26]殷红艳,佟慧丽,刘丹,等.脂肪酸对牛骨骼肌卫星细胞分化的影响[J].中国民康医学,2015(14):157-157.
[27]丁志丽,曹访,罗娜,等.花生四烯酸对日本沼虾肝胰腺细胞脂质代谢基因表达的影响[J].动物营养学报,2017(2):536-546.
[28]陆伦根,曾民德,李继强,等.花生四烯酸和亚油酸对肝星状细胞增殖的影响[J].世界华人消化杂志,1999(1):10-12.
[29]杨立杰,杨在宾,姜淑贞.必需脂肪酸对动物的功能及其需要量再认识[J].饲料工业,2016(12):31-33.
[2]贺文琦,赵魁,高丰.动物病理解剖学[M].北京:科学出版社,2013,6:38-39.
[3]霍春芬,霍慧兰,朱红霞.中药换药法治愈难愈性溃疡126例[J].中国美容医学,2012(12):285-286.
[4]付小兵,程飙.伤口愈合的新概念[J].中国实用外科杂志2005,25(1):29-32.
[5] Smith A, Willis E, Chan V, et al. Mesenchymal stem cells inducedermal fibroblast responses to injury[J]. Exp Cell Res, 2010, 316:48-54.
[6] Shojaku H, Takakura H, Okabe M, et al. Effect of hyperdryamniotic membrane patches attached over the bony surface ofmastoid cavities in canal wall down tympanoplasty[J].Laryngoscope, 2011, 121:1953-1957.
[7]霍双枝,施萍,庞希宁.人羊膜负载人羊膜间充质干细胞对SD大鼠皮肤创面愈合的影响[J].中国医学科学院报,2011,33:611-614.
[8] Moalla R D, Ben K S, Ksouda M K, et al. Evaluation of WoundHealing Properties of Grape Seed, Sesame, and Fenugreek Oils[J].Evidence-Based Complementary and Alternative Medicine,2016(4):1-12.
[9] Mori H M, Kawanami H, Kawahata H, et al. Wound healingpotential of lavender oil by acceleration of granulation and woundcontraction through induction of TGF-βin a rat model[J]. BMCComplementary and Alternative Medicine,2016,16(1).
[10] Frank S, Stallmeyer B, Kampfer H, et al. Leptin enhances wound re-epithelialization and constilutes a dinect function of leptin in shinrepair[J]. J Clin Invest, 2000, 106:501-509.
[11] Ring B D, Scully S, Davis C R, et al. Systemically and topicallyadministered lept in both accelerate wound healing in diabeticoh/obmice[J]. Endocrinology. 2000, 141:446-449.
[12]张凯,司艺玲,林季,等.瘦素及其抗体对小鼠皮肤创伤的修复作用[J].标记免疫分析与临床,2008(4):231-233,247.
[13]刘晓玉,王瑞,张涛,等.人脂肪间充质干细胞对皮肤创伤修复的作用[J].基础医学与临床,2013(11):1377-1381.
[14] Rosa AdS, Bandeira L G, Monte-Alto-Costa A, et al.Supplementation with olive oil, but not fish oil, improves cutaneouswound healing in stressed mice[J]. Wound Repair andRegeneration. 2014,22(4):537-47.
[15] Cardoso C R, Favoreto S Jr, Oliveira L L, et al. Oleic acidmodulation of the immune response in wound healing:a newapproach for skin repair[J]. Immunobiology 2011,216:409-415.
[16] Rodrigues H G, Vinolo M A, Magdalon J, et al. Oral administrationof oleic or linoleic acid accelerates the inflammatory phase ofwound healing[J]. J Invest Dermatol 2012,132:208-215.
[17] Pereira L M, Hatanaka E, Martins E F, et al. Effect of oleic andlinoleic acids on the inflammatory phase of wound healing in rats[J]. Cell Biochem Funct 2008,26:197-204.
[18] Rodrigues H G, Vinolo M A, Magdalon J, et al. Dietary free oleicand linoleic acid enhances neutrophil function and modulates theinflammatory response in rats[J]. Lipids 2010,45:809-819.
[19] Li H L, Chen L P, Hu Y H, et al. Crocodile Oil Enhances CutaneousBurn Wound Healing and Reduces Scar Formation in Rats.Academic Emergency Medicine.
[20]杨瑞,李亚洁.创伤修复动物模型的建立[J].护理研究,2003(23):1369-1370.
[21]程会军,陈明亮,孙自强,等.硫化氢对小鼠皮肤伤口愈合的促进作用[J].河南大学学报:医学版,2015(1):32-35.
[22]张彩丽,张俊霞,李瑞琴.小鼠皮肤组织石蜡切片制作经验[J].中国现代医药杂志,2016(5):91-92.
[23]尹玉鑫,施振声,曾申明.犬脂肪干细胞在犬皮肤创伤修复中的作用[J].犬业科技,2016(4):18-22.
[24]魏振东,郝泗城,孙建华,等.中药去腐生肌散等促进动物实验性皮肤溃疡修复作用的病理观察[J].天津医药,1990(09):547-548,577.
[25]郝薇,李福海,王玉林. Omega-3多不饱和脂肪酸对角化细胞及成纤维细胞的作用[J].山东大学学报,2012,50(9):50-54.
[26]殷红艳,佟慧丽,刘丹,等.脂肪酸对牛骨骼肌卫星细胞分化的影响[J].中国民康医学,2015(14):157-157.
[27]丁志丽,曹访,罗娜,等.花生四烯酸对日本沼虾肝胰腺细胞脂质代谢基因表达的影响[J].动物营养学报,2017(2):536-546.
[28]陆伦根,曾民德,李继强,等.花生四烯酸和亚油酸对肝星状细胞增殖的影响[J].世界华人消化杂志,1999(1):10-12.
[29]杨立杰,杨在宾,姜淑贞.必需脂肪酸对动物的功能及其需要量再认识[J].饲料工业,2016(12):31-33.