时间因素对PAMAM诱导牙本质再矿化的影响
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摘要
目的:探讨不同时间羧基改性的聚酰胺-胺型(PAMAM)对牙本质再矿化的影响。方法:选取20个牙本质块,随机分成A、B、C、D、E组,经37%的磷酸凝胶酸蚀后,A组作为空白对照不做任何处理,B~E组放置于羧基改性的PAMAM溶液中处理20min,再放置于饱和Ca(OH)2溶液中预处理30min,然后分别浸泡在人工唾液中矿化1,3,5,7d。利用扫描电镜(SEM)评估牙本质再矿化的效果。结果:SEM显示各组牙本质小管的矿化程度不同,随矿化时间延长,小管内矿化物逐渐形成,矿化物与牙本质小管的结合逐渐紧密。结论:PAMAM诱导牙本质形成的矿化物随着时间的增加而增加,提示了PAMAM在牙本质敏感的治疗方面有一定的潜在应用价值。
Objective:To evaluate the effect of carboxyl-modified polyamidoamine dendrimers(PAMAM)on the reminer alization of human dentin in different time.Methods:Twenty dentin discs were randomly divided into groups A,B,C,D and E.After demineralized with 37% phosphoric acid,group A was served as the control,and groups B to E were first treated with carboxyl-modified PAMAM for 20minutes,and then pretreatment with satu-rated Ca(OH)2solution for 30minutes before immersing into artificial saliva for a period of time(1,3,5and 7d)respectively.The dentin discs were characterized by scanning electron microscope(SEM).Results:SEM showed that the results were different in different groups.The minerals in dentinal tubules were formed gradually and the combination of them close gradually with the time.Conclusion:The experimental results showed that polyamidoamine dendrimers induce remineralization of dentin obviously with the time.Consequently,this strategy is valuable for developing a new method to treat dentin hype-rsensitivity.
Objective:To evaluate the effect of carboxyl-modified polyamidoamine dendrimers(PAMAM)on the reminer alization of human dentin in different time.Methods:Twenty dentin discs were randomly divided into groups A,B,C,D and E.After demineralized with 37% phosphoric acid,group A was served as the control,and groups B to E were first treated with carboxyl-modified PAMAM for 20minutes,and then pretreatment with satu-rated Ca(OH)2solution for 30minutes before immersing into artificial saliva for a period of time(1,3,5and 7d)respectively.The dentin discs were characterized by scanning electron microscope(SEM).Results:SEM showed that the results were different in different groups.The minerals in dentinal tubules were formed gradually and the combination of them close gradually with the time.Conclusion:The experimental results showed that polyamidoamine dendrimers induce remineralization of dentin obviously with the time.Consequently,this strategy is valuable for developing a new method to treat dentin hype-rsensitivity.
引文
[1]樊明文,周学东.牙体牙髓病学[M].3版.北京:人民卫生出版社,2008:144.
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[3]王天达,王磊,冯海兰.聚酰胺一胺型树枝状分子对牙本质小管的封闭作用[J].中华口腔医学杂志,2012,47(z1):153-157.
[4]Li JH,Yang JJ.Bioinspired intrafibrillar minerali-zation of human dentine by PAMAM dendrimer[J].Biomaterials,2013,34:6 738-6 747.
[5]Brannstrom M.Sensitivity of dentine[J].Oral Surg Oral med Oral Pathol,1966,21(4):517-526.
[6]Tomalia DA,Baker HA.Starburst dendritic macromolecules[J].Polym J,1985,17(1):117-132.
[7]Chen H,Banaszak Holl M,Orr BG,et al.Interaction of dendr-imers(artificial proteins)with biological hydroxyapatite crystals[J].Journal of Dental Research,2003,82(6):443-448.
[8]Wu D,Yang J,Li J,et al.Hydroxyapatite-anchored dendrimer for in situ remineralization of human tooth enamel[J].Biomaterials,2013,34:5 036-5 047.
[9]刘彦,谢方方.树枝状聚合物诱导牙釉质仿生再矿化的体外实验研究[J].临床口腔医学杂志,2013,29(4):381-383.
[10]Chen L,Liang KN,Li JS,et al.Regeneration of biomimetic hydroxyapatite on etched human enamel by anionic PAMAM template in vitro[J].Archives of Oral Biology,2013,58:975-980.
[2]荣文笙,胡德渝,冯希平,等.我国城市地区成人牙本质敏感的流行病学调查[J].中华口腔医学杂志,2010,45(3):141-145.
[3]王天达,王磊,冯海兰.聚酰胺一胺型树枝状分子对牙本质小管的封闭作用[J].中华口腔医学杂志,2012,47(z1):153-157.
[4]Li JH,Yang JJ.Bioinspired intrafibrillar minerali-zation of human dentine by PAMAM dendrimer[J].Biomaterials,2013,34:6 738-6 747.
[5]Brannstrom M.Sensitivity of dentine[J].Oral Surg Oral med Oral Pathol,1966,21(4):517-526.
[6]Tomalia DA,Baker HA.Starburst dendritic macromolecules[J].Polym J,1985,17(1):117-132.
[7]Chen H,Banaszak Holl M,Orr BG,et al.Interaction of dendr-imers(artificial proteins)with biological hydroxyapatite crystals[J].Journal of Dental Research,2003,82(6):443-448.
[8]Wu D,Yang J,Li J,et al.Hydroxyapatite-anchored dendrimer for in situ remineralization of human tooth enamel[J].Biomaterials,2013,34:5 036-5 047.
[9]刘彦,谢方方.树枝状聚合物诱导牙釉质仿生再矿化的体外实验研究[J].临床口腔医学杂志,2013,29(4):381-383.
[10]Chen L,Liang KN,Li JS,et al.Regeneration of biomimetic hydroxyapatite on etched human enamel by anionic PAMAM template in vitro[J].Archives of Oral Biology,2013,58:975-980.