根域限制对“红地球”葡萄果实糖含量和蔗糖代谢相关酶活性的影响
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摘要
以3年生的"红地球"葡萄为试材,测定其果实中葡萄糖、果糖和蔗糖含量以及蔗糖代谢相关酶-可溶性酸性转化酶(SAI)、可溶性中性转化酶(SNI)、细胞壁转化酶(CBAI)和蔗糖合成酶(SSⅡ)活性,以研究葡萄果实糖类积累与蔗糖代谢相关酶活性的关系。结果表明:果实成熟期根域限制栽培果实的可溶性总糖含量明显高于对照;与对照组相比,根域限制组葡萄糖含量提高了12.8%,果糖含量提高了3.4%,蔗糖含量提高了20.0%,总糖含量提高了10.8%。说明根域限制栽培显著提高了"红地球"葡萄果实成熟期的葡萄糖、蔗糖和总糖含量,糖含量的提高受到了SAI、SNI、CBAI、SSⅡ活性的调控;根域限制栽培显著提高了转色期SAI和CBAI的活性和成熟期SNI和SSⅡ的活性。
The content of sucrose,glucose,fructose and the activities of sucrose-metabolizin enzymes(soluble acid invertase,soluble neutral invertase,cell wall-bound acid invertase,sucrose synthase and sucrose phosphate synthase)in the grape berry of‘Red Globe'were measured.This study aimed to explore the effect of root restriction on sugar metabolism and the activities of sucrose-metabolizin enzymes.The results showed that,the total soluble sugar content under root restriction was significantly higher than the control during grape berry maturing.Compared with the control group,the glucose content under root restriction increased by 12.8%,fructose content increased by 3.4%,sucrose content increased by 20.0%,total sugar content increased by10.8%.It showed that,root restriction significantly improved the content of glucose,sucrose and total sugar during‘Red Globe'grape berry maturing The addition of soluble sugar content was controlled by activities of SAI,SNI,CBAI,SSⅡ.Root restriction greatly increased the activities of SAI and CBAI at the turning color stage and increased the activities of SNI and SSⅡat the late stage of fruit development.
The content of sucrose,glucose,fructose and the activities of sucrose-metabolizin enzymes(soluble acid invertase,soluble neutral invertase,cell wall-bound acid invertase,sucrose synthase and sucrose phosphate synthase)in the grape berry of‘Red Globe'were measured.This study aimed to explore the effect of root restriction on sugar metabolism and the activities of sucrose-metabolizin enzymes.The results showed that,the total soluble sugar content under root restriction was significantly higher than the control during grape berry maturing.Compared with the control group,the glucose content under root restriction increased by 12.8%,fructose content increased by 3.4%,sucrose content increased by 20.0%,total sugar content increased by10.8%.It showed that,root restriction significantly improved the content of glucose,sucrose and total sugar during‘Red Globe'grape berry maturing The addition of soluble sugar content was controlled by activities of SAI,SNI,CBAI,SSⅡ.Root restriction greatly increased the activities of SAI and CBAI at the turning color stage and increased the activities of SNI and SSⅡat the late stage of fruit development.
引文
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[2]IMAI S,OKAMOTO G,ENDO M.Effect of dense planting and root system control on attaining greater early production and fruit stability of tetraploid grapes[J].Bull Hiroshima Fruit Tree Expt Sta,1987(12):1-9.
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[14]谢兆森,李渤,许文平,等,根域限制对葡萄果实发育和主要营养物质含量的影响[J].植物生理学通讯,2008,44(5):927-930.
[15]谢周,孙兴民,张萌,等.根域限制对‘宝满’葡萄光合特性及糖代谢的影响[J].中国农学通报,2012,28(22):190-196.
[16]谢兆森.根域限制对葡萄果实发育、源库器官及其输导组织结构的影响[D].上海:上海交通大学,2010.
[17]卢彩玉,郑小艳,贾惠娟,等.根域限制对‘巨玫瑰’葡萄果实可溶性糖含量及相关代谢酶活的影响[J].园艺学报,2011,38(5):825-832.
[18]WANG S P,OKAMOTO G,HIRANO K,et al.Effect of root restriction on vine nutrition of Kyoho gape from dormanoy to anthesis[J].J Japan Soe Hort Sci,1997,66(suppl.1):86-87.
[19]WANG S P,OKAMOTO G,HIRANO K,et al.Effecs of rootingzone restriction on the change carbohydrates and nitrogenous compounds in Kyoho grapevines during winter dormancy and early shoot growth[J].J Japan Soc Hort Sci,1998,67:577-582.
[20]WANG S P,OKAMOTO G,HIRANO K,et al.Effects of restricted rooting volume on vine growth and berry development of‘Kyoho’grapevines[J].American Journal of Enology and Viticulture,2001,52(3):248-253.
[21]IMAI S,FUJIWARA T,TANAKA S,et al.Effect of soil moisture on vine growth and fruit production of‘Kyoho’grapes growing on restricted rooting volume[J].Environ Control in Biol,1991,29:133-140.
[22]RICHARD D,ROWE R N.Effects of root restrietion,root pruning and 6-benyzlaminopurine on the growth of peach seedlings[J].Ann Bot,1977,41:729-740.
[23]STOLL M B,LOVEYS B,DRY P.Hormonal changes inducde by partial rootzone drying of irrigated grapevine[J].J Exp Bot,2000,51:1627-1634.
[2]IMAI S,OKAMOTO G,ENDO M.Effect of dense planting and root system control on attaining greater early production and fruit stability of tetraploid grapes[J].Bull Hiroshima Fruit Tree Expt Sta,1987(12):1-9.
[3]SAWANO Y.Planting types and problems in satsuma mandarin[J].Japan Soc Hort Sci,1993,62(suppl.2):13-25.
[4]MYERS S C.Root restriction of apple and peach with in-ground fabric containers[J].Acta Hori,1992,322:215-219.
[5]HOMMI Y,SAKAKIBARA M,KIMURA T.Capacity of root zone watering and fertilizer application for peach trees in container culture[J].Research Bulletin of the Aichiken Agriculture Research Center,1995,27:251-258.
[6]WEBSTER A D,ATKINSON J C,VAUGHAN J S.Controlling the shoot growth and cropping of sweet cherry trees using root pruning or root restriclion techniques[J].Acta Hort,1997,451:643-649.
[7]TANIKUTI T.Rooting-zone restriction for satsuma mandarin[J].Agri and Hort,1993,68:490-496.
[8]王世平,张才喜,罗菊花,等.果树根域限制栽培研究进展[J].果树学报,2002,19(5):298-301.
[9]王博.根域限制促进鲜食葡萄果皮花色营合成的机制研究[D].上海:上海交通大学,2013.
[10]薛应龙.植物生理学实验手册[M].上海:上海科学技术出版社,1985:206-225.
[11]赵智中,张上隆,徐昌杰,等.蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用[J].园艺学报,2001,28(2):112-118.
[12]王永章,王小芳,张大鹏.苹果果实转化酶的种类和特性研究[J].中国农业大学学报,2001,6(5):9-14.
[13]王银川,刘效义,汪泽鹏.宁夏贺兰山东麓气候条件及品种区划[J].宁夏科技,2002(1):36-37.
[14]谢兆森,李渤,许文平,等,根域限制对葡萄果实发育和主要营养物质含量的影响[J].植物生理学通讯,2008,44(5):927-930.
[15]谢周,孙兴民,张萌,等.根域限制对‘宝满’葡萄光合特性及糖代谢的影响[J].中国农学通报,2012,28(22):190-196.
[16]谢兆森.根域限制对葡萄果实发育、源库器官及其输导组织结构的影响[D].上海:上海交通大学,2010.
[17]卢彩玉,郑小艳,贾惠娟,等.根域限制对‘巨玫瑰’葡萄果实可溶性糖含量及相关代谢酶活的影响[J].园艺学报,2011,38(5):825-832.
[18]WANG S P,OKAMOTO G,HIRANO K,et al.Effect of root restriction on vine nutrition of Kyoho gape from dormanoy to anthesis[J].J Japan Soe Hort Sci,1997,66(suppl.1):86-87.
[19]WANG S P,OKAMOTO G,HIRANO K,et al.Effecs of rootingzone restriction on the change carbohydrates and nitrogenous compounds in Kyoho grapevines during winter dormancy and early shoot growth[J].J Japan Soc Hort Sci,1998,67:577-582.
[20]WANG S P,OKAMOTO G,HIRANO K,et al.Effects of restricted rooting volume on vine growth and berry development of‘Kyoho’grapevines[J].American Journal of Enology and Viticulture,2001,52(3):248-253.
[21]IMAI S,FUJIWARA T,TANAKA S,et al.Effect of soil moisture on vine growth and fruit production of‘Kyoho’grapes growing on restricted rooting volume[J].Environ Control in Biol,1991,29:133-140.
[22]RICHARD D,ROWE R N.Effects of root restrietion,root pruning and 6-benyzlaminopurine on the growth of peach seedlings[J].Ann Bot,1977,41:729-740.
[23]STOLL M B,LOVEYS B,DRY P.Hormonal changes inducde by partial rootzone drying of irrigated grapevine[J].J Exp Bot,2000,51:1627-1634.