伪狂犬病病毒变异株Fujian-LY对生长育肥猪的致病性研究
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摘要
将6头80日龄伪狂犬病病毒(PRV) g B、g E抗体均为阴性的生长育肥猪随机分成对照组和感染组,每组3头。感染组试验猪通过颈部肌肉接种Fujian-LY株2m L,对照组猪经相同途径接种等体积的DMEM,每日观察记录试验猪的发病及死亡情况,试验期为7 d。结果显示,感染组育肥猪临床症状表现明显,在感染1 d后体温均开始升高,随着病情的发展,表现咳嗽、喘气、流鼻涕等典型的呼吸道症状,至试验结束未出现死亡。病理剖检显示,与对照组相比,感染育肥猪除脑部病变明显外,心、肝、脾、肺、肾等主要器官组织均未出现明显的肉眼可见病变。组织病理学检查结果显示,PRV Fujian-LY株已对感染育肥猪的多个主要器官组织造成了较为严重的病理损伤:脑实质中小血管扩张充血,血管周围有淋巴细胞包围的"血管套"现象;肝索紊乱、肝细胞坏死;免疫器官中淋巴细胞减少等。同时采用PCR及免疫组织化学方法对这些组织进行检测均呈PRV阳性。g B、g E抗体检测结果显示,感染猪在感染后第7天PRV g B、g E抗体均开始转阳。上述结果表明,PRV Fujian-LY株对生长育肥猪具有较强的致病性,在发病猪体内分布广泛。
Six 80-day-old growing-finishing pigs,which pseudorabies virus(PRV) g B and g E antibodies were all negative,were randomly divided into control group(3 pigs) and infection group(3 pigs).The pigs in the infection group were inoculated with the PRV Fujian-LY strain through the neck muscle,and the control group pigs were inoculated with equal volume DMEM by the same way.And the incidence and mortality of the experimental pigs were observed daily for 7 days.The results showed that the clinical symptoms of the infected pigs were obvious,the body temperature began to increase at 1 day post infection.With the development of the disease,typical respiratory symptoms such as cough,gasping and runny nose were observed,and no death occurred until the end of the experiment.Pathological necropsy revealed that compared with the control group, the main organs such as the heart,liver,spleen,lung and kidney of the infected pigs showed no obvious visible lesions except for the brain.The histopathological examination showed that the PRV Fujian-LY strain had caused more serious histopathological damage in the major organ tissues of the infected pigs such as parenchymal small angiectasis hyperemia and the phenomenon of blood vessels surrounded by the lymphocytes in the brain tissue, liver hepatic cord disorder and hepatocyte necrosis in the liver tissue,and lymphocyte reduction in the immunity organs.At the same time,the detection results of PRV antigen in these tissues by PCR and immunohistochemistry were all positive. The detection results of g B and g E antibodies showed that the PRV g B and g E antibodies of the infected pigs began to turn positive at the 7 th day after infection.The above results indicated that the PRV Fujian-LY strain has strong pathogenicity to growing-finishing pigs and it was widely distributed in the infected pigs.
Six 80-day-old growing-finishing pigs,which pseudorabies virus(PRV) g B and g E antibodies were all negative,were randomly divided into control group(3 pigs) and infection group(3 pigs).The pigs in the infection group were inoculated with the PRV Fujian-LY strain through the neck muscle,and the control group pigs were inoculated with equal volume DMEM by the same way.And the incidence and mortality of the experimental pigs were observed daily for 7 days.The results showed that the clinical symptoms of the infected pigs were obvious,the body temperature began to increase at 1 day post infection.With the development of the disease,typical respiratory symptoms such as cough,gasping and runny nose were observed,and no death occurred until the end of the experiment.Pathological necropsy revealed that compared with the control group, the main organs such as the heart,liver,spleen,lung and kidney of the infected pigs showed no obvious visible lesions except for the brain.The histopathological examination showed that the PRV Fujian-LY strain had caused more serious histopathological damage in the major organ tissues of the infected pigs such as parenchymal small angiectasis hyperemia and the phenomenon of blood vessels surrounded by the lymphocytes in the brain tissue, liver hepatic cord disorder and hepatocyte necrosis in the liver tissue,and lymphocyte reduction in the immunity organs.At the same time,the detection results of PRV antigen in these tissues by PCR and immunohistochemistry were all positive. The detection results of g B and g E antibodies showed that the PRV g B and g E antibodies of the infected pigs began to turn positive at the 7 th day after infection.The above results indicated that the PRV Fujian-LY strain has strong pathogenicity to growing-finishing pigs and it was widely distributed in the infected pigs.
引文
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[20]郑仲华,吴艳红,张仕云,等.广东地区PRV流行毒株gE基因的进化分析及对育肥猪的致病性研究[J].中国畜牧兽医文摘,2017,33(5):65.ZHENG Zhong-hua,WU Yan-hong,ZHANG Shi-yun,et al.Evolution analysis of gE gene of PRV prevalent strain in Guangdong and its pathogenicity to finishing pigs[J].Chinese Abstract of Animal Husbandry and Veterinary Medicine,2017,33(5):65.(in Chinese)
[21]薛协超,张丽,路万平,等.育肥猪伪狂犬病毒、大肠杆菌混合感染的诊断与防控分析[J].黑龙江畜牧兽医,2017(12):137-139.XUE Xie-chao,ZHANG Li,LU Wan-ping,et al.Diagnosis and control of mixed infection of pseudorabies virus and Escherichia coli in fattening pigs[J].Heilongjiang Animal Science and Veterinary Medicine,2017(12):137-139.(in Chinese)
[2]杨小燕,戴爱玲,李晓华.闽西地区猪伪狂犬野毒株感染的血清学调查[J].安徽农业大学学报,2008,35(4):540-543.YANG Xiao-yan,DAI Ai-ling,LI Xiao-hua,et al.Serological survey of infection by swine wild PRV in western Fujian Province[J].Journal of Anhui Agricultural University,2008,35(4):540-543.(in Chinese)
[3] AN Tong-qing,PENG Jin-mei,TIAN Zhi-jun,et al.Pseudorabies virus variant in Bartha-K61-vaccinated pigs,China,2012[J].Emerging Infectious Diseases,2013,19(11):1749-1755.
[4] LUO Yu-zi,LI Na,CONG Xin,et al.Pathogenicity and genomic characterization of a pseudorabies virus variant isolated from Bartha-K61-vaccinated swine population in China[J].Veterinary Microbiology,2014,174(1-2):107-115.
[5] TONG Wu,LIU Fei,ZHENG Hao,et al.Emergence of a pseudorabies virus variant with increased virulence to piglets[J].Veterinary Microbiology,2015,181(3-4):236-240.
[6] FAN Jin-dai, ZHENG Xi-duo, ZHANG Guan-qun, et al.Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong Province of Southern China during2013-2014[J].Journal of Veterinary Science,2015,17(3):369-375.
[7] HU Dong-fang,ZHANG Zhen-dong,L譈Liu,et al.Outbreak of variant pseudorabies virus in Bartha-K61-vaccinated piglets in central Shandong Province,China[J].Journal of Veterinary Diagnostic Investigation,2015,27(5):600-605.
[8] YU Xiu-ling,ZHOU Zhi,HU Dong-mei,et al.Pathogenic pseudorabies virus,China,2012[J].Emerging Infectious Diseases,2014,20(1):102-104.
[9]方奎,陈基萍.生长育肥猪伪狂犬病的防控[J].中国猪业,2018,13(2):60-62.FANG Kui,CHEN Ji-ping.Prevention and control of pseudorabies in growing and fattening pigs[J].China Swine Industry,2018,13(2):60-62.(in Chinese)
[10]刘亚彬.新流行猪伪狂犬病的临床发病规律和防控措施[J].中国猪业,2015(Z1):112-116.LIU Ya-bin.Clinical characteristics and prevention and control of new epidemic pig pseudorabies[J].China Swine Industry,2015(Z1):112-116.(in Chinese)
[11]宁文一,陈松昌,万文忠,等.一例生长育肥猪发生猪伪狂犬病的诊治[J].江西畜牧兽医杂志,2015(3):31-33.NING Wen-yi,CHEN Song-chang,WAN Wen-zhong,et al.Diagnosis and treatment of pseudorabies in a growing and fattening pig[J].Jiangxi Journal of Animal Husbandry&Veterinary Medicine,2015(3):31-33.(in Chinese)
[12]方奎,陈基萍.育肥猪群猪伪狂犬病的防控[J].中国猪业,2016,11(8):29-32.FANG Kui,CHEN Ji-ping.Prevention and control of pseudorabies in fattening pigs[J].China Swine Industry,2016,11(8):29-32.(in Chinese)
[13]杨汉春.猪伪狂犬病的流行现状与特点[J].猪业科学,2016,33(1):38.YANG Han-chun.Epidemic status and characteristics of pseudorabies in pigs[J].Swine Industry Science,2016,33(1):38.(in Chinese)
[14]童光志.再度流行的伪狂犬病毒有何变异[J].北方牧业,2018(13):17.TONG Guang-zhi.What is the variation of repopular pseudorabies virus[J].Beifang Muye,2018(13):17.(in Chinese)
[15]范克伟,戴爱玲,李晓华,等.猪源伪狂犬病病毒福建龙岩分离株的鉴定及其TK基因和gE基因的分子特征[J].中国兽医科学,2015,45(10):1021-1030.FAN Ke-wei,DAI Ai-ling,LI Xiao-hua,et al.Identification of pseudorabies virus isolated from swine in Longyan of Fujian Province and molecular characteristics of TK and gE genes of the isolate[J]. Chinese Veterinary Science, 2015, 45(10):1021-1030.(in Chinese)
[16]范克伟,杨小燕,杨守深,等.闽西地区猪伪狂犬病病毒变异株gE基因新变异序列鉴定[J].中国兽医学报,2015,35(12):1903-1910.FAN Ke-wei,YANG Xiao-yan,YANG Shou-shen,et al.Genetic variation of gE gene of porcine pseudorabies virus variants isolated in Western Fujian Province[J].Chinese Journal of Veterinary Science,2015,35(12):1903-1910.(in Chinese)
[17]马兴杰,罗亚坤,崔尚金.猪伪狂犬病病毒(PRV-JL株)的分离与鉴定[J].养猪,2014(5):109-112.MA Xing-jie,LUO Ya-kun,CUI Shang-jin.Isolation and identification of porcine pseudorabies virus(PRV-JL strain)[J].Swine Production,2014(5):109-112.(in Chinese)
[18]范克伟,戴爱玲,吴德峰,等.猪伪狂犬病毒变异株(Fujian-LY)对免疫仔猪的致病性研究[J].中国兽医学报,2015,35(11):1727-1734.FAN Ke-wei,DAI Ai-ling,WU De-feng,et al.Pathogenicity of variant strain Fujian-LY of pseudorabies virus in immune piglets[J].Chinese Journal of Veterinary Science,2015,35(11):1727-1734.(in Chinese)
[19]刘涛,王瑞,王举才.南阳地区育肥猪群猪伪狂犬病的诊断与防控[J].黑龙江畜牧兽医,2014(12):77-78.LIU Tao,WANG Rui,WANG Ju-cai.Diagnosis and control of pseudorabies in fattening pigs in Nanyang area[J].Heilongjiang Animal Science and Veterinary Medicine,2014(12):77-78.(in Chinese)
[20]郑仲华,吴艳红,张仕云,等.广东地区PRV流行毒株gE基因的进化分析及对育肥猪的致病性研究[J].中国畜牧兽医文摘,2017,33(5):65.ZHENG Zhong-hua,WU Yan-hong,ZHANG Shi-yun,et al.Evolution analysis of gE gene of PRV prevalent strain in Guangdong and its pathogenicity to finishing pigs[J].Chinese Abstract of Animal Husbandry and Veterinary Medicine,2017,33(5):65.(in Chinese)
[21]薛协超,张丽,路万平,等.育肥猪伪狂犬病毒、大肠杆菌混合感染的诊断与防控分析[J].黑龙江畜牧兽医,2017(12):137-139.XUE Xie-chao,ZHANG Li,LU Wan-ping,et al.Diagnosis and control of mixed infection of pseudorabies virus and Escherichia coli in fattening pigs[J].Heilongjiang Animal Science and Veterinary Medicine,2017(12):137-139.(in Chinese)