影响脂肪组织工程中细胞外基质支架的相关因素研究进展
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摘要
支架作为组织工程三要素之一,它主要是作为细胞临时附着点,其降解速度应与组织再生速度相匹配,待组织重建后支架可降解排泄,不对人体造成任何损害。目前研究的组织工程支架主要有天然可降解高分子材料、人工合成的可降解高分子材料、无机材料等,脂肪组织脱细胞基质支架作为近年来的研究热门,因其来源丰富、取材方便,受到研究者们的青睐,本文就影响脂肪组织细胞外基质支架的相关因素综述如下。
As one of the three elements of tissue engineering, scaffold is mainly used as a temporary attachment point of cells. Its degradation rate should match the rate of tissue regeneration. After the tissue is reconstructed, the scaffold can be degraded and excreted without causing any damage to the human body. Currently, the tissue engineering scaffolds studied mainly include natural degradable polymer materials, synthetic degradable polymer materials, inorganic materials, etc. In recent years. adipose tissue decellularized matrix scaffolds have been studied popularly due to the advantages of its sources and convenience in materials. This article will review the correlative factors affecting the extracellular matrix scaffold of adipose tissue.
As one of the three elements of tissue engineering, scaffold is mainly used as a temporary attachment point of cells. Its degradation rate should match the rate of tissue regeneration. After the tissue is reconstructed, the scaffold can be degraded and excreted without causing any damage to the human body. Currently, the tissue engineering scaffolds studied mainly include natural degradable polymer materials, synthetic degradable polymer materials, inorganic materials, etc. In recent years. adipose tissue decellularized matrix scaffolds have been studied popularly due to the advantages of its sources and convenience in materials. This article will review the correlative factors affecting the extracellular matrix scaffold of adipose tissue.
引文
[1]Brown BN,Freund JM,Han L,et al.Comparison of three methods for the derivation of a biologic scaffold composed of adipose tissue extracellular matrix[J].Tissue Eng Part C Methods,2011,17(4):411-421.
[2]察鹏飞,高建华,陈阳,等.人脂肪组织细胞外基质支架的构建[J].中华整形外科杂志,2012,28(1):55-60.
[3]Negishi J,Funamoto S,Kimura T,et al.Porcine radial artery decellularization by high hydrostatic pressure[J].Tissue Eng Regen Med,2015,9(11):E144-E151.
[4]Wang P,Li C,Zhang R,et al.Various methods of preparing acellular tissueengineered xenogeneic valve stents[J].Crter,2009,13(16):3041-3044.
[5]Sabetkish S,Kajbafzadeh AM,Sabetkish N,et al.Whole-organ tissue engineering:decellularization and recellularization of threedimensional matrix liver scaffolds[J].J Biomed Mater Res(Part A),2015,103(4):1498-1508.
[6]Elder BD,Eleswarapu SV,Athanasiou KA.Extraction techniques for the decellularization of tissue engineered articular cartilage constructs[J].Biomaterials,2009,30(22):3749-3756.
[7]孙亨,王艳霞,吴江.组织脱细胞方法的研究进展[J].四川解剖学杂志,2011,19(4):24-30.
[8]Turner AEB,Yu C,Bianco J,et al.The performance of decellularized adipose tissue microcarriers as an inductive substrate for human adipose-derived stem cells[J].Biomaterials,2012,33(18):4490-4499.
[9]Yu C,Bianco J,Brown C,et al.Porous decellularized adipose tissue foams for soft tissue regeneration[J].Biomaterials,2013,34(13):3290-3302.
[10]武欣,谷涌泉,段红永,等.利用脱细胞血管基质体外构建小口径组织工程血管[J].中国实验动物学报,2010,18(5):377-382.
[11]陆军,肖学钧,陈欣欣.比较牛颈静脉去细胞处理实验方法[J].岭南心血管病杂志,2008,14(6):440-443.
[12]崔小平,张永红,薛军.胰蛋白酶法和Triton X-100法脱猪软骨细胞的比较[J].中国组织工程研究与临床康复,2009,13(46):23.
[13]韩啸,蒋雄刚,徐鹏,等.聚乙二醇和胰蛋白酶用于猪动脉管道去细胞效果比较[J].华中科技大学学报:医学版,2007,36(4):551-553.
[14]Wang P,Li C,Zhang R,et al.Various methods of preparing acellular tissue-engineered xenogeneic valve stents[J].Crter,2009,13(16):3041-3044.
[15]Gilbert TW,Sellaro TL,Badylak SF.Decellularization of tissues and organs[J].Biomaterials,2006,27(19):3675-3683.
[16]Freytes DO,Stoner RM,Badylak SF.Uniaxial and biaxial properties of terminally sterilized porcine urinary bladder matrix scaffolds[J].JBiomed Mater Res(Part B),2008,84(2):408-414.
[17]Flynn LE.The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells[J].Biomaterials,2010,31(17):4715-4724.
[18]Suto K,Urabe K,Naruse K,et al.Repeated freeze-thaw cycles reduce the survival rate of osteocytes in bone-tendon constructs without affecting the mechanical properties of tendons[J].Cell Tissue Bank,2012,13(1):71-80.
[19]Jung HJ,Vangipuram G,Fisher MB,et al.The effects of multiple freeze-thaw cycles on the biomechanical properties of the human bone‐patellar tendon‐bone allograft[J].J Orthop Res,2011,29(8):1193-1198.
[20]Allen KD,Athanasiou KA.A surface-regional and freeze-thaw characterization of the porcine temporomandibular joint disc[J].Ann Biomed Eng,2005,33(7):951-962.
[21]Lu H,Hoshiba T,Kawazoe N,et al.Comparison of decellularization techniques for preparation of extracellular matrix scaffolds derived from three‐dimensional cell culture[J].J Biomed Mater Res A,2012,100(9):2507-2516.
[22]范雪娇,田春祥,傅月荷,等.脂肪脱细胞基质的制备和初步评价[J].中国修复重建外科杂志,2014,28(3):377-383.
[23]宋玫,刘毅,惠玲.利用脂肪脱细胞基质构建组织工程化脂肪的实验研究[J].解放军医药杂志,2016,28(1):30-34.
[24]田亚菲.人脂肪组织细胞外基质支架的制备与检测及其在大鼠软组织缺损模型中降解的实验研究[D].兰州:兰州大学,2016.
[25]Lumpkins SB,Pierre N,McFetridge PS.A mechanical evaluation of three decellularization methods in the design of a xenogeneic scaffold for tissue engineering the temporomandibular joint disc[J].Acta Biomater,2008,4(4):808-816.
[26]Crapo PM,Gilbert TW,Badylak SF.An overview of tissue and whole organ decellularization processes[J].Biomaterials,2011,32(12):3233-3243.
[27]Lovekamp JJ,Simionescu DT,Mercuri JJ,et al.Stability and function of glycosaminoglycans in porcine bioprosthetic heart valves[J].Biomaterials,2006,27(8):1507-1518.
[28]Schenke-Layland K,Vasilevski O,Opitz F,et al.Impact of decellularization of xenogeneic tissue on extracellular matrix integrity for tissue engineering of heart valves[J].J Struct Biol,2003,143(3):201-208.
[29]Wu I,Nahas Z,Kimmerling KA,et al.An injectable adipose matrix for soft tissue reconstruction[J].Plast Reconstr Surg,2012,129(6):1247.
[30]Engler AJ,Sen S,Sweeney H L,et al.Matrix elasticity directs stem cell lineage specification[J].Cell,2006,126(4):677-689.
[31]Birgersdotter A,Sandberg R,Ernberg I.Gene expression perturbation in vitro-a growing case for three-dimensional(3D)culture systems[J].Semin Cancer Biol,2005,15(5):405-412.
[32]Kulangara K,Yang Y,Yang J,et al.Nanotopography as modulator of human mesenchymal stem cell function[J].Biomaterials,2012,33(20):4998-5003.
[33]Oh SH,Park IK,Kim JM,et al.In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method[J].Biomaterials,2007,28(9):1664-1671.
[34]张洁,徐烈.冻干支架孔隙形态的影响因素[J].低温工程,2002,23(2):38-43.
[35]Courtman DW,Errett BF,Wilson GJ.The role of crosslinking in modification of the immune response elicited against xenogenic vascular acellular matrices[J].J Biomed Mater Res(Part BAppl),2001,55(4):576-586.
[36]Brown BN,Valentin JE,Stewart-Akers AM,et al.Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular component[J].Biomaterials,2009,30(8):1482-1491.
[37]Spiller KL,Anfang RR,Spiller KJ,et al.The role of macrophage phenotype in vascularization of tissue engineering scaffolds[J].Biomaterials,2014,35(15):4477-4488.
[38]Hu C,Ding Y,Chen J,et al.Basic fibroblast growth factor stimulates epithelial cell growth and epithelial wound healing in canine corneas[J].Vet Ophthalmol,2009,12(3):170-175.
[39]Gordon S.Alternative activation of macrophages[J].Nat Rev Immunol,2003,3(1):23.
[40]Brown BN,Londono R,Tottey S,et al.Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical meshmaterials[J].Act a Biomater,2012,8(3):978-987.
[41]Choi JS,Yang HJ,Kim BS,et al.Fabrication of porous extracellular matrix scaffolds from human adipose tissue[J].Tissue Eng Part CMethods,2009,16(3):387-396.
[42]Choi JS,Yang HJ,Kim BS,et al.Human extracellular matrix(ECM)powders for injectable cell delivery and adipose tissue engineering[J].J Control Release,2009,139(1):2-7.
[43]吕荣.周围神经损伤修复及功能恢复评价[J].中国组织工程研究与临床康复,2011,15(8):1454-1458.
[44]唐骁,叶小龙,黄江龙,等.新型纳米仿生-防粘连复合型疝补片的力学性能及细胞相容性[J].中国组织工程研究,2018,22(6):902-907.
[2]察鹏飞,高建华,陈阳,等.人脂肪组织细胞外基质支架的构建[J].中华整形外科杂志,2012,28(1):55-60.
[3]Negishi J,Funamoto S,Kimura T,et al.Porcine radial artery decellularization by high hydrostatic pressure[J].Tissue Eng Regen Med,2015,9(11):E144-E151.
[4]Wang P,Li C,Zhang R,et al.Various methods of preparing acellular tissueengineered xenogeneic valve stents[J].Crter,2009,13(16):3041-3044.
[5]Sabetkish S,Kajbafzadeh AM,Sabetkish N,et al.Whole-organ tissue engineering:decellularization and recellularization of threedimensional matrix liver scaffolds[J].J Biomed Mater Res(Part A),2015,103(4):1498-1508.
[6]Elder BD,Eleswarapu SV,Athanasiou KA.Extraction techniques for the decellularization of tissue engineered articular cartilage constructs[J].Biomaterials,2009,30(22):3749-3756.
[7]孙亨,王艳霞,吴江.组织脱细胞方法的研究进展[J].四川解剖学杂志,2011,19(4):24-30.
[8]Turner AEB,Yu C,Bianco J,et al.The performance of decellularized adipose tissue microcarriers as an inductive substrate for human adipose-derived stem cells[J].Biomaterials,2012,33(18):4490-4499.
[9]Yu C,Bianco J,Brown C,et al.Porous decellularized adipose tissue foams for soft tissue regeneration[J].Biomaterials,2013,34(13):3290-3302.
[10]武欣,谷涌泉,段红永,等.利用脱细胞血管基质体外构建小口径组织工程血管[J].中国实验动物学报,2010,18(5):377-382.
[11]陆军,肖学钧,陈欣欣.比较牛颈静脉去细胞处理实验方法[J].岭南心血管病杂志,2008,14(6):440-443.
[12]崔小平,张永红,薛军.胰蛋白酶法和Triton X-100法脱猪软骨细胞的比较[J].中国组织工程研究与临床康复,2009,13(46):23.
[13]韩啸,蒋雄刚,徐鹏,等.聚乙二醇和胰蛋白酶用于猪动脉管道去细胞效果比较[J].华中科技大学学报:医学版,2007,36(4):551-553.
[14]Wang P,Li C,Zhang R,et al.Various methods of preparing acellular tissue-engineered xenogeneic valve stents[J].Crter,2009,13(16):3041-3044.
[15]Gilbert TW,Sellaro TL,Badylak SF.Decellularization of tissues and organs[J].Biomaterials,2006,27(19):3675-3683.
[16]Freytes DO,Stoner RM,Badylak SF.Uniaxial and biaxial properties of terminally sterilized porcine urinary bladder matrix scaffolds[J].JBiomed Mater Res(Part B),2008,84(2):408-414.
[17]Flynn LE.The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells[J].Biomaterials,2010,31(17):4715-4724.
[18]Suto K,Urabe K,Naruse K,et al.Repeated freeze-thaw cycles reduce the survival rate of osteocytes in bone-tendon constructs without affecting the mechanical properties of tendons[J].Cell Tissue Bank,2012,13(1):71-80.
[19]Jung HJ,Vangipuram G,Fisher MB,et al.The effects of multiple freeze-thaw cycles on the biomechanical properties of the human bone‐patellar tendon‐bone allograft[J].J Orthop Res,2011,29(8):1193-1198.
[20]Allen KD,Athanasiou KA.A surface-regional and freeze-thaw characterization of the porcine temporomandibular joint disc[J].Ann Biomed Eng,2005,33(7):951-962.
[21]Lu H,Hoshiba T,Kawazoe N,et al.Comparison of decellularization techniques for preparation of extracellular matrix scaffolds derived from three‐dimensional cell culture[J].J Biomed Mater Res A,2012,100(9):2507-2516.
[22]范雪娇,田春祥,傅月荷,等.脂肪脱细胞基质的制备和初步评价[J].中国修复重建外科杂志,2014,28(3):377-383.
[23]宋玫,刘毅,惠玲.利用脂肪脱细胞基质构建组织工程化脂肪的实验研究[J].解放军医药杂志,2016,28(1):30-34.
[24]田亚菲.人脂肪组织细胞外基质支架的制备与检测及其在大鼠软组织缺损模型中降解的实验研究[D].兰州:兰州大学,2016.
[25]Lumpkins SB,Pierre N,McFetridge PS.A mechanical evaluation of three decellularization methods in the design of a xenogeneic scaffold for tissue engineering the temporomandibular joint disc[J].Acta Biomater,2008,4(4):808-816.
[26]Crapo PM,Gilbert TW,Badylak SF.An overview of tissue and whole organ decellularization processes[J].Biomaterials,2011,32(12):3233-3243.
[27]Lovekamp JJ,Simionescu DT,Mercuri JJ,et al.Stability and function of glycosaminoglycans in porcine bioprosthetic heart valves[J].Biomaterials,2006,27(8):1507-1518.
[28]Schenke-Layland K,Vasilevski O,Opitz F,et al.Impact of decellularization of xenogeneic tissue on extracellular matrix integrity for tissue engineering of heart valves[J].J Struct Biol,2003,143(3):201-208.
[29]Wu I,Nahas Z,Kimmerling KA,et al.An injectable adipose matrix for soft tissue reconstruction[J].Plast Reconstr Surg,2012,129(6):1247.
[30]Engler AJ,Sen S,Sweeney H L,et al.Matrix elasticity directs stem cell lineage specification[J].Cell,2006,126(4):677-689.
[31]Birgersdotter A,Sandberg R,Ernberg I.Gene expression perturbation in vitro-a growing case for three-dimensional(3D)culture systems[J].Semin Cancer Biol,2005,15(5):405-412.
[32]Kulangara K,Yang Y,Yang J,et al.Nanotopography as modulator of human mesenchymal stem cell function[J].Biomaterials,2012,33(20):4998-5003.
[33]Oh SH,Park IK,Kim JM,et al.In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method[J].Biomaterials,2007,28(9):1664-1671.
[34]张洁,徐烈.冻干支架孔隙形态的影响因素[J].低温工程,2002,23(2):38-43.
[35]Courtman DW,Errett BF,Wilson GJ.The role of crosslinking in modification of the immune response elicited against xenogenic vascular acellular matrices[J].J Biomed Mater Res(Part BAppl),2001,55(4):576-586.
[36]Brown BN,Valentin JE,Stewart-Akers AM,et al.Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular component[J].Biomaterials,2009,30(8):1482-1491.
[37]Spiller KL,Anfang RR,Spiller KJ,et al.The role of macrophage phenotype in vascularization of tissue engineering scaffolds[J].Biomaterials,2014,35(15):4477-4488.
[38]Hu C,Ding Y,Chen J,et al.Basic fibroblast growth factor stimulates epithelial cell growth and epithelial wound healing in canine corneas[J].Vet Ophthalmol,2009,12(3):170-175.
[39]Gordon S.Alternative activation of macrophages[J].Nat Rev Immunol,2003,3(1):23.
[40]Brown BN,Londono R,Tottey S,et al.Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical meshmaterials[J].Act a Biomater,2012,8(3):978-987.
[41]Choi JS,Yang HJ,Kim BS,et al.Fabrication of porous extracellular matrix scaffolds from human adipose tissue[J].Tissue Eng Part CMethods,2009,16(3):387-396.
[42]Choi JS,Yang HJ,Kim BS,et al.Human extracellular matrix(ECM)powders for injectable cell delivery and adipose tissue engineering[J].J Control Release,2009,139(1):2-7.
[43]吕荣.周围神经损伤修复及功能恢复评价[J].中国组织工程研究与临床康复,2011,15(8):1454-1458.
[44]唐骁,叶小龙,黄江龙,等.新型纳米仿生-防粘连复合型疝补片的力学性能及细胞相容性[J].中国组织工程研究,2018,22(6):902-907.