不同浓度镉胁迫诱导乌龟脂质过氧化损伤的研究
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摘要
目的:探究不同浓度镉胁迫对乌龟脂质过氧化损伤的影响。方法:健康实验龟随机分为对照组、7.5 mg/kg组、15mg/kg组和30 mg/kg组。7.5 mg/kg组、15 mg/kg组和30 mg/kg组分别腹腔注射7.5 mg/kg、15 mg/kg、30 mg/kg灭菌的氯化镉溶液染毒,对照组腹腔注射等容量的生理盐水。结果:与对照组比较,7.5 mg/kg组、15 mg/kg组和30 mg/kg组乌龟血浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和谷胱甘肽(GSH)含量在染毒后1 w、2 w、3 w均不同程度降低,而丙二醛(MDA)含量有不同程度的升高。结论:实验龟腹腔注射7.5 mg/kg、15 mg/kg、30 mg/kg氯化镉溶液引起了氧化损伤。
Objective:The study investigated the lipid peroxidation lesion of freshwater turtle Chinemys reevesii exposed to different concentration cadmium. Methods:The experimental turtles were randomly divided into control group,7.5 mg/kg group,15 mg/kg group and 30 mg/kg group,and were exposed to 0 mg/kg,7.5 mg/kg,15 mg/kg,30 mg/kg sterile cadmium chloride and normal saline separately by intraperitoneal injection. Results:Compared with the control group,SOD,CAT activities and content of GSH in plasma of the 7.5 mg/kg,15 mg/kg,30 mg/kg groups were decreased in 1 week,2 weeks and 3 weeks. However,content of MDA was increased. Conclusion:Experiment turtles exposed to cadmium at the concentration of 7.5 mg/kg,15 mg/kg,30 mg/kg were led to oxidative Lesion.
Objective:The study investigated the lipid peroxidation lesion of freshwater turtle Chinemys reevesii exposed to different concentration cadmium. Methods:The experimental turtles were randomly divided into control group,7.5 mg/kg group,15 mg/kg group and 30 mg/kg group,and were exposed to 0 mg/kg,7.5 mg/kg,15 mg/kg,30 mg/kg sterile cadmium chloride and normal saline separately by intraperitoneal injection. Results:Compared with the control group,SOD,CAT activities and content of GSH in plasma of the 7.5 mg/kg,15 mg/kg,30 mg/kg groups were decreased in 1 week,2 weeks and 3 weeks. However,content of MDA was increased. Conclusion:Experiment turtles exposed to cadmium at the concentration of 7.5 mg/kg,15 mg/kg,30 mg/kg were led to oxidative Lesion.
引文
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[14]Mruk DD,Silvestrini B,Mo M,et al.Antioxidant superoxide dismutase-a review:its function,regulation in the testis,and role in male fertility[J].Contraception,2002(65):305-311.
[15]Fomazier RF,Ferreira RR,Viteria AP,et al.Effects of cadmium on antioxidant enzyme activities in sugar cane[J].Biol Plant,2002(45):91-97.
[16]Cuypers A,Plusquin M,Remans T,et al.Cadmium stress:an oxidative challenge[J].Biometals,2010(23):927-940.
[17]Rashid K,Sinha K,Sil PC.An update on oxidative stress-mediated organ pathophysiology[J].Food Chem Toxicol,2013(62):584-600.
[2]朱善良,陈龙,高伟,等.镉致大鼠睾丸脂质过氧化及酶活性变化研究[J].中国公共卫生,2003(6):79-81.
[3]王宁,程宁,闫铭峰,等.大鼠急性羰基镍中毒各脏器谷胱甘肽过氧化物酶水平态观察[J].中国职业医学,2012,9(4):293-296.
[4]Zhao EM.Studies on taxonomy and distribution of Chinese chelonians[J].Sichan Journal of Zoology,1997,15(Suppl):1-26.
[5]黄徐根,吴孝兵,张志强,等.乌龟胃肠胰系统内分泌细胞的免疫组织化学研究[J].水生生物学报,2005,29(3):291-298.
[6]Wu H,Xuan RJ,Li YJ,et al.Biochemical,histological and ultrastructural alterations of the alimentary system in the freshwater crab Sinopotamon henanense subchronically exposed to cadmium[J].Ecotoxicology,2014(23):65-75.
[7]Gilgun-Sherki Y,Rosenbaum Z,Melamed E,et al.Antioxidant therapy in acute central nervous system injury:current state[J].Pharmacol Rev,2002(54):271-284.
[8]Sarkar S,Yadav P,Bhatnagar D.Lipid peroxidative damage on cadmium exposure and alternations in antioxidant systems in rat erythrocytes:a study with relation to time[J].Biometals,1998(11):153-157.
[9]Lawal AO,Lawal AF,Olongodudu A,et al.Antioxidant effects of heated garlic juice on cadmium induced liver damage in rats as compared to ascorbic acid[J].J Toxicol Sci,2011,36(5):549-557.
[11]Hajiani M,Razi F,Golestani A,et al.Time-and dose-dependent differential regulation of copper-zinc superoxide dismutase and manganese superoxide dismutase enzymatic activity and m RNA level by vitamin E in rat blood cells[J].Redox Rep,2012(17):101-107.
[12]Sevcikova M,Modra H,Slaninova A,et al.Metals as a cause of oxidative stress in fish:a review[J].Vet Med-Czech,2011,56(11):537-546.
[13]Kratz F,Sohn HY,Gloe T,et al.NAD(P)H oxidase-dependent platelet superoxide anion release increases platelet recruitment[J].Blood,2002,100(3):917-924.
[14]Mruk DD,Silvestrini B,Mo M,et al.Antioxidant superoxide dismutase-a review:its function,regulation in the testis,and role in male fertility[J].Contraception,2002(65):305-311.
[15]Fomazier RF,Ferreira RR,Viteria AP,et al.Effects of cadmium on antioxidant enzyme activities in sugar cane[J].Biol Plant,2002(45):91-97.
[16]Cuypers A,Plusquin M,Remans T,et al.Cadmium stress:an oxidative challenge[J].Biometals,2010(23):927-940.
[17]Rashid K,Sinha K,Sil PC.An update on oxidative stress-mediated organ pathophysiology[J].Food Chem Toxicol,2013(62):584-600.