凝结芽孢杆菌对感染肠炎沙门氏菌蛋鸡肠道形态、抗氧化能力及沙门氏菌定植的影响
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摘要
为研究凝结芽孢杆菌对感染肠炎沙门氏菌蛋鸡肠道形态、抗氧化能力及沙门氏菌定植的影响,将400只沙门氏菌阴性蛋鸡随机分成4组,每2组分在环境条件相同的2个安全隔离舍,每组设5次重复,每个重复20只鸡。对照组(I组和III组)饲喂基础饲粮,试验组(II组和IV组)在基础饲粮上添加2.5×1010cfu/kg凝结芽孢杆菌。饲喂1周后,试验组连续两天定量口服沙门氏菌菌液,对照组口服等量无菌PBS溶液,饲养3周。结果表明:1)日粮添加凝结芽孢杆菌极显著增加十二指肠绒毛宽度和绒毛吸收表面积(PBC <0.01);添加凝结芽孢杆菌可显著增加空肠绒毛长度和绒毛长度/隐窝深度比值(PBC<0.05)。沙门氏菌极显著降低空肠隐窝深度(PSE <0.01),显著降低绒毛宽度(PSE<0.05),极显著增加绒毛长度/隐窝深度比值(PSE<0.01)。2)日粮添加凝结芽孢杆菌后,沙门氏菌与凝结芽孢杆菌对血浆SOD有显著交互作用(PSE×BC <0.05),沙门氏菌可以显著增加血浆SOD和TAOC(PSE<0.05);添加凝结芽孢杆菌显著降低血浆中SOD和MDA的含量(PBC<0.05)。3)在攻毒后第1天、第14天和第21天,日粮添加凝结芽孢杆菌可显著降低盲肠沙门氏菌拷贝数(PBC<0.05);在整个试验过程中观察发现,凝结芽孢杆菌可显著降低盲肠沙门氏菌拷贝数(PBC <0.05)。综上,产蛋后期蛋鸡感染肠炎沙门氏菌后,日粮添加凝结芽孢杆菌可改善肠道形态,提升其抗氧化能力,降低盲肠沙门氏菌定植数量,缓解病源性感染应激,保证了蛋鸡机体健康。
This study evaluated the effects of Bacillus coagulans(BC)on the intestinal morphology,plasma antioxidation capacity,pro-inflammatory cytokines and colonization of caecum salmonella in S.enteritidis(SE)infected laying hens.A total of 400 Salmonella-negative,healthy hens were randomly divided into 4 treatment groups with 100 in each group,among which every two groups were allotted to same biosafety isolated sheds.Each group consisted of 5 replicates with 20 hens per replicate.Different dietary treatments were:I,basal diet;II,basal diet+oral Salmonella administration;III,basal diet+2.5 x1010 CFU/kg B.coagulans,IV,basal diet+2.5 x1010 CFU/kg B.coagulans +oral Salmonella administration.All hens in III and IV groups were orally challenged with Salmonella at day 7 and day 8 after the initiation of experiment,whereas hens in other two groups were treated with the same amountof sterile PBS.The results showed that:1)B.coagulans significantly increased(PBC<0.05)the duodenum villus width and villus surface area.Meanwhile,B.coagulans significantly increased(PBC<0.05)the jejunum villus height and VH:CD ratio.However,Salmonella significantly decreased(PSE <0.01)the jejunum crypt depth,villus width and VH:CD ratio(PSE <0.05).2)B.coagulans significantly decreased(PBC<0.05)the plasma of superoxide dismutase(SOD)activity and malondialdehyde(MDA).Salmonella significantly increased(PSE<0.05)SOD activity and total antioxidant capacity(T-AOC).3)During the post-SE-challenge period,B.coagulans significantly reduced(PEF<0.05)Salmonella copies at day 1,14 and 21 post inoculation(dpi).Meanwhile,from dpi 1 to dpi 21,the Salmonella copies in layers fed with B.coagulans was lower than(PEF <0.05)hens fed with basal diet.It is concluded that B.coagulans can improve intestinal health,alleviate the oxidative stress caused by Salmonella infection,reduce the colonization of Salmonellain caecum,relieve the stress of pathogenic infection and promote the health of birds.
This study evaluated the effects of Bacillus coagulans(BC)on the intestinal morphology,plasma antioxidation capacity,pro-inflammatory cytokines and colonization of caecum salmonella in S.enteritidis(SE)infected laying hens.A total of 400 Salmonella-negative,healthy hens were randomly divided into 4 treatment groups with 100 in each group,among which every two groups were allotted to same biosafety isolated sheds.Each group consisted of 5 replicates with 20 hens per replicate.Different dietary treatments were:I,basal diet;II,basal diet+oral Salmonella administration;III,basal diet+2.5 x1010 CFU/kg B.coagulans,IV,basal diet+2.5 x1010 CFU/kg B.coagulans +oral Salmonella administration.All hens in III and IV groups were orally challenged with Salmonella at day 7 and day 8 after the initiation of experiment,whereas hens in other two groups were treated with the same amountof sterile PBS.The results showed that:1)B.coagulans significantly increased(PBC<0.05)the duodenum villus width and villus surface area.Meanwhile,B.coagulans significantly increased(PBC<0.05)the jejunum villus height and VH:CD ratio.However,Salmonella significantly decreased(PSE <0.01)the jejunum crypt depth,villus width and VH:CD ratio(PSE <0.05).2)B.coagulans significantly decreased(PBC<0.05)the plasma of superoxide dismutase(SOD)activity and malondialdehyde(MDA).Salmonella significantly increased(PSE<0.05)SOD activity and total antioxidant capacity(T-AOC).3)During the post-SE-challenge period,B.coagulans significantly reduced(PEF<0.05)Salmonella copies at day 1,14 and 21 post inoculation(dpi).Meanwhile,from dpi 1 to dpi 21,the Salmonella copies in layers fed with B.coagulans was lower than(PEF <0.05)hens fed with basal diet.It is concluded that B.coagulans can improve intestinal health,alleviate the oxidative stress caused by Salmonella infection,reduce the colonization of Salmonellain caecum,relieve the stress of pathogenic infection and promote the health of birds.
引文
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[11]赵树平,包维臣,高鹏飞,姚国强,郭霄,张和平.凝结芽孢杆菌的特性及研究进展[J].家畜生态学报,2014,35(2):6-11,20.Zhao S P,Bao W C,Gao P F,Yao G Q,Guo X,Zhang H P.Study on characteristics of Bacillus coagulans[J].Acta Ecologiae Animalis Domastici,2014,35(2):6-11,20(in Chinese)
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[14]罗丽萍.屎肠球菌对感染沙门氏菌肉鸡生产性能、肠道形态和微生物数量的影响[D].中国农业大学硕士学位论文,2015Luo L P. Influences ofEnterococcus faecium with Salmonella typhimurium infection on broilers′performace,intesinal morphology and gut microflora[D], China Agricultral University,2015(in Chinese)
[15]王全溪,林滉,贾洪强,张志霆,王长康.乳酸菌素对肉鸡血清生化指标和抗氧化功能的影响[J].动物营养学报,2012,24(01):131-136Wang Q X,Lin H,Jia H Q,Zhang Z T,Wang C K.Effect of Lactobacillin on serum biochemical indices and antioxidant function in broilers[J], Chinese Journal of Animal Nutrition,2012,24(1):131-136(in Chinese)
[16]丁贤,唐维可,张善夫,李卓佳,林黑着,杨铿.芽孢杆菌对草鱼消化酶活性及血清生化指标的影响[J].广东农业科学,2012,39(17):121-124.Ding X,Tang W K,Zhang S F,Li Z J,Lin H Z,Yang J.Effects of Bacillus subtilis on digestive enzymes activities and serum biochemical indices of Ctenopharyngodon idellus[J].Guangdong Agricultural Sciences,2012,39(17):121-124(in Chinese)
[17]林丽花,柯芙容,詹湉湉,许丽惠,王全溪,王长康,黄树文.凝结芽孢杆菌对黄羽肉鸡生产性能、血清生化指标及抗氧化功能的影响[J].动物营养学报,2014,26(12):3806-3813Lin L H,Ke F R,Zhan T T,Xu L H,Wang Q X,Wang C K, Huang S W. Effect ofBacillus coagulans on performance,serum biochemical indices and antioxidant function of yellow broilers[J],Chinese Journal of Animal Nutrition,2014,26(12):3806-3813(in Chinese)
[18] Rajput I R,Li W F,Li Y L,Jian L,Wang M Q.Application of probiotic(Bacillus subtilis)to enhance immunity,antioxidation,digestive enzymes activity and hematological profile of Shaoxing duck[J].Pakistan Veterinary Journal,2013,33(1):69-72
[19] Bialka K L,Demirci A,Knabel S J,Patterson P H,Puri V M.Efficacy of electrolyzed oxidizing water for the microbial safety and quality of eggs[J].Poultry Science,2004,83(12):2071-2078
[20] Dunkley K D,Callaway T R,Chalova V I,Mcreynolds J L,Hume M E,Dunkley C S,Kubena L F,Nisbet D J,Ricke S C. FoodborneSalmonella, ecologyintheavian gastrointestinal tract[J].Anaerobe,2009,15(1-2):26-35
[2] Barrow P A,Lovell M A.Experimental infection of egg-laying hens with Salmonella enteritidis phage type 4[J].Avian Pathology.1991,20(2):335-348
[3] Gyles C L.Antimicrobial resistance in selected bacteria from poultry[J].Animal Health Research Reviews,2008,9(2):149-158
[4]王人悦,郑琳琳,佟永薇.益生菌制品的应用及前景展望[J].食品研究与开发,2013,34(11):128-130Wang R Y,Zheng L L,Tong Y H.Application and prospect of probiotic products[J].Food Research and Development.2013,34(11):128-130(in Chinese)
[5]赵朋超,王建华,权春善,范圣第.枯草芽孢杆菌抗菌肽生物合成的研究进展[J].中国生物工程杂志,2010,30(10):108-113Zhao P C,Wang J H,Quan S C,Fan S D.Progress on biosynthesis of antimicrobial peptides from Bacillus subtilis[J].China Biotechnology,2010,30(10):108-113(in Chinese)
[6]罗建杰.日粮添加不同益生菌对肉仔鸡益生作用分子机制研究[D].中国农业科学院硕士学位论文,2013Luo J J.The probiotic mechansim of several diatery probiotics on broiler[D].Chinese Academy of Agricultral Sciences,2013(in Chinese)
[7] Samli H E,Dezcan S,Koc F,Ozduven M L,Okur A A,Senkoylu N.Effects of Enterococcus faeciumsupplementation and floor type on performance,morphology of erythrocytes and intestinal microbiota in broiler chickens[J]. British Poultry Science,2010,51(4):564-568
[8] Chen C Y,Tsen H Y,Lin C L,Yu B,Chen C S.Oral administration of a combination of select lactic acid bacteria strains to reduce the Salmonellainvasion and inflammation of broiler chicks[J].Poultry Science,2012,91(9):2139-2147
[9] Higgins J P,Higgins S E,Wolfenden A D,Henderson S N,Torresrodriguez A,Vicente J L,Hargis B M,Tellez G.Effect of lactic acid bacteria probiotic culture treatment timing on Salmonella enteritidis in neonatal broilers[J].Poultry Science,2010,89(2):243-247
[10] Menconi A,Wolfenden A D,Shivaramaiah S,Terraes J C,Urbano T,Kuttel J,Kremer C,Hargis B M,Tellez G.Effect of lactic acid bacteria probiotic culture for the treatment of Salmonella enterica serovar Heidelberg in neonatal broiler chickens and turkey poults[J].Poultry Science,2011,90(3):561-565
[11]赵树平,包维臣,高鹏飞,姚国强,郭霄,张和平.凝结芽孢杆菌的特性及研究进展[J].家畜生态学报,2014,35(2):6-11,20.Zhao S P,Bao W C,Gao P F,Yao G Q,Guo X,Zhang H P.Study on characteristics of Bacillus coagulans[J].Acta Ecologiae Animalis Domastici,2014,35(2):6-11,20(in Chinese)
[12] Kernéis S,Bogdanova A,Kraehenbuhl J P,Pringault E.Conversion by Peyer′s patch lymphocytes of human enterocytes into M cells that transport bacteria[J].Science,1997,277(5328):949-952
[13]孙焕林,刘艳丰,王品,许追,侯广田,赵洁.凝结芽孢杆菌对黄羽肉鸡生长性能、肠道功能及肉品质的影响[J].石河子大学学报,2014,32(3):307-312Sun H L,Liu Y F,Wang P,Xu Z,Hou G T,Zhao J.Effect of Bacillus coagulans on growth performance,intestinal function and its meat quality of Yellow-Feathered chicken[J].Journal of Shihezi University,2014(in Chinese)
[14]罗丽萍.屎肠球菌对感染沙门氏菌肉鸡生产性能、肠道形态和微生物数量的影响[D].中国农业大学硕士学位论文,2015Luo L P. Influences ofEnterococcus faecium with Salmonella typhimurium infection on broilers′performace,intesinal morphology and gut microflora[D], China Agricultral University,2015(in Chinese)
[15]王全溪,林滉,贾洪强,张志霆,王长康.乳酸菌素对肉鸡血清生化指标和抗氧化功能的影响[J].动物营养学报,2012,24(01):131-136Wang Q X,Lin H,Jia H Q,Zhang Z T,Wang C K.Effect of Lactobacillin on serum biochemical indices and antioxidant function in broilers[J], Chinese Journal of Animal Nutrition,2012,24(1):131-136(in Chinese)
[16]丁贤,唐维可,张善夫,李卓佳,林黑着,杨铿.芽孢杆菌对草鱼消化酶活性及血清生化指标的影响[J].广东农业科学,2012,39(17):121-124.Ding X,Tang W K,Zhang S F,Li Z J,Lin H Z,Yang J.Effects of Bacillus subtilis on digestive enzymes activities and serum biochemical indices of Ctenopharyngodon idellus[J].Guangdong Agricultural Sciences,2012,39(17):121-124(in Chinese)
[17]林丽花,柯芙容,詹湉湉,许丽惠,王全溪,王长康,黄树文.凝结芽孢杆菌对黄羽肉鸡生产性能、血清生化指标及抗氧化功能的影响[J].动物营养学报,2014,26(12):3806-3813Lin L H,Ke F R,Zhan T T,Xu L H,Wang Q X,Wang C K, Huang S W. Effect ofBacillus coagulans on performance,serum biochemical indices and antioxidant function of yellow broilers[J],Chinese Journal of Animal Nutrition,2014,26(12):3806-3813(in Chinese)
[18] Rajput I R,Li W F,Li Y L,Jian L,Wang M Q.Application of probiotic(Bacillus subtilis)to enhance immunity,antioxidation,digestive enzymes activity and hematological profile of Shaoxing duck[J].Pakistan Veterinary Journal,2013,33(1):69-72
[19] Bialka K L,Demirci A,Knabel S J,Patterson P H,Puri V M.Efficacy of electrolyzed oxidizing water for the microbial safety and quality of eggs[J].Poultry Science,2004,83(12):2071-2078
[20] Dunkley K D,Callaway T R,Chalova V I,Mcreynolds J L,Hume M E,Dunkley C S,Kubena L F,Nisbet D J,Ricke S C. FoodborneSalmonella, ecologyintheavian gastrointestinal tract[J].Anaerobe,2009,15(1-2):26-35