尖叶假龙胆对脂多糖诱导脓毒症模型大鼠急性肾损伤的保护作用研究
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摘要
目的观察尖叶假龙胆对脂多糖(LPS)诱导脓毒症大鼠急性肾损伤的保护作用。方法通过单次腹腔注射LPS(7 mg/kg)制备模型,以地塞米松为阳性对照药,通过观察与检测大鼠的一般情况、尿量、血清尿素氮(BUN)和肌酐(Scr)及尿中肾组织肾损伤分子-1(KIM-1)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)水平,考察尖叶假龙胆对大鼠急性肾损伤保护作用。结果造模后24 h时,尖叶假龙胆组、地塞米松组血清Scr和BUN、尿液中KIM-1与NGAL含量与模型组差异有统计学意义(P <0.05)。结论尖叶假龙胆对LPS诱导脓毒症模型大鼠急性肾损伤模型有一定的保护作用,其机制可能与抑制KIM-1与NGAL蛋白有关。
Objective: To investigate the protective effect of Gentianella Acuta on LPS-induced acute kidney injury in sepsis rats. Methods: The model was established by single intraperitoneal injection of LPS(7 mg/kg).Dexamethasone was used as positive control drug. The protective effects of Gentianella acuta on acute renal injury in rats were studied by observing and detecting the general condition,urine volume,BUN,Scr,KIM-1 and NGAL levels in urine. Results: 24 h after model establishment,serum Scr,BUN and levels of urine KIM-1 and NGAL of other groups were significantly different from those in the model group(P < 0.05). Conclusion: Gentianella acuta has a protective effect on LPS-induced acute renal injury in rats,which may be related to the inhibition of KIM-1 and NGAL protein.
Objective: To investigate the protective effect of Gentianella Acuta on LPS-induced acute kidney injury in sepsis rats. Methods: The model was established by single intraperitoneal injection of LPS(7 mg/kg).Dexamethasone was used as positive control drug. The protective effects of Gentianella acuta on acute renal injury in rats were studied by observing and detecting the general condition,urine volume,BUN,Scr,KIM-1 and NGAL levels in urine. Results: 24 h after model establishment,serum Scr,BUN and levels of urine KIM-1 and NGAL of other groups were significantly different from those in the model group(P < 0.05). Conclusion: Gentianella acuta has a protective effect on LPS-induced acute renal injury in rats,which may be related to the inhibition of KIM-1 and NGAL protein.
引文
[1]吴琼.尖叶假龙胆化学成分及酮类成分药代动力学研究[D].哈尔滨:黑龙江中医药大学,2016.
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[15] Kuchroo VK. The TIM gene family:emerging roles in immunity and disease[J]. Nat Rev Immunol,2003,3(6):454-462.
[16] Han WK. Urinary biomarkers in the early diagno sis of acute kidney injury[J]. Kidney Int,2008,73(7):863-869.
[17]刘畅,饶向荣,安成,等.急性肾损伤中医证候要素及其与肾损伤分子-1的相关性研究[J].中国中西医结合肾病杂志,2010,11(2):138-140.
[18]李一飞,姚广涛.急性肾损伤中肾小管损伤标志物的研究进展[J].中国实验方剂学杂志,2011,17(23):279-284.
[19] Mishra J. Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury[J]. J Am Soc Nephrol,2003,14(10):2534-2543.
[20]吴昱,李晓玫.急性肾损伤相关生物标志物的研究进展[J].中国病理生理杂志,2010,26(8):1650-1653,1661.
[21]杨洁.比较不同剂量地塞米松对脓毒症导致的小鼠急性肾脏损伤的保护作用[D].济南:山东大学,2013.
[2]张雪.尖叶假龙胆血清药物化学及DMB药代动力学研究[D].哈尔滨:黑龙江中医药大学,2016.
[3]刘中原,李延平.细菌脂多糖的生物活性及作用机制[J].医学综述,2010,16(2):166-169.
[4]刘清泉,李雁,寇兰俊.多脏器功能失常综合征中医病因病机及治法探讨[J].北京中医药大学学报,2000,23(3):57-58.
[5]刘清泉,张晓云,孔立,等.高热(脓毒症)中医诊疗专家共识意见[J].中国中医急症,2014,23(11):1961-1963.
[6] Doi,K. Animal models of sepsis and sepsis-induced kidney injury[J]. J Clin Invest,2009,119(10):2868-2878.
[7] Angus DC,Pereira CA,Silva E. Epidemiology of severe sepsis around the world[J]. Endocr Metab Immune Disord Drug Targets,2006,6(2):207-212.
[8] Ostermann M. Kinetics of urinary cell cycle arrest markers for acute kidney injury following exposure to potential renal insults[J]. Crit Care Med,2018,46(3):375-383.
[9]狄浩然,卢幼然,吴彦青,等.脓毒症急性肾损伤的发病机制和中医治疗[J].世界中医药,2018,13(8):2079-2083,2089.
[10] Bagshaw SM,George C,Bellomo R. Early acute kidney injury and sepsis:a multicentre evaluation[J]. Crit Care,2008,12(2):R47.
[11] Hoste EA,Schurgers M.Epidemiology of acute kidney injury:how big is the problem[J]. Crit Care Med,2008,36(4Suppl):S146-151.
[12]姚咏明.脓毒症动物模型的选择与评价[J].继续医学教育,2008,22(1):28-31.
[13] Rittirsch D. Immunodesign of experimental sepsis by cecal ligation and puncture[J]. Nat Protoc,2009,4(1):31-36.
[14] Song,S.Serum cystatin C in mouse models:a reliable and precise marker for renal function and superior to serum creatinine[J]. Nephrol Dial Transplant,2009,24(4):1157-1161.
[15] Kuchroo VK. The TIM gene family:emerging roles in immunity and disease[J]. Nat Rev Immunol,2003,3(6):454-462.
[16] Han WK. Urinary biomarkers in the early diagno sis of acute kidney injury[J]. Kidney Int,2008,73(7):863-869.
[17]刘畅,饶向荣,安成,等.急性肾损伤中医证候要素及其与肾损伤分子-1的相关性研究[J].中国中西医结合肾病杂志,2010,11(2):138-140.
[18]李一飞,姚广涛.急性肾损伤中肾小管损伤标志物的研究进展[J].中国实验方剂学杂志,2011,17(23):279-284.
[19] Mishra J. Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury[J]. J Am Soc Nephrol,2003,14(10):2534-2543.
[20]吴昱,李晓玫.急性肾损伤相关生物标志物的研究进展[J].中国病理生理杂志,2010,26(8):1650-1653,1661.
[21]杨洁.比较不同剂量地塞米松对脓毒症导致的小鼠急性肾脏损伤的保护作用[D].济南:山东大学,2013.