海带酶解产物对海参生长及其免疫相关因子的影响
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摘要
采用生物酶降解技术处理海带,研究海带酶解产物对海参的生长及其免疫相关因子表达的影响,探讨其作为饵料添加剂的应用前景。结果表明,酶解海带寡糖和酶解海带泥在海参生长方面均有促进作用,其中分别添加10%和25%的海带寡糖和酶解海带泥的效果最佳(海参生长率分别为76.60%和40.30%)。另外,酶解海带寡糖可以提高海参体壁组织中免疫相关因子(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP))的活性,通过减少丙二醛(MDA)的产生保护机体免受氧化损伤(添加10%海带寡糖使MDA释放量降低至0.002 2μmol/mg)。
Laminaria japonica was treated by biological enzymes degradation technology, and the effects of the L. japonica enzymatic hydrolysate on the growth and immune-related factors expression of sea cucumber was studied, and to explore the application prospect of the L. japonica enzymatic hydrolysate as a bait additive. The results showed that the L. japonica enzymatic hydrolysate including oligosaccharides and mud could promote the growth of sea cucumber. The effect of adding oligosaccharide 10% and mud 25% was the optimal, and the growth rate of sea cucumber was 76.60%and 40.30%, respectively. In addition, the enzymatic hydrolysis oligosaccharides of L. japonica could increase the activity of immune-related factors including superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), alkaline phosphatase(ACP), acid phosphatase(AKP) in the perisome tissue of sea cucumber, and decrease the production of malondialdehyde(MDA), which could protect the body from oxidative damage(MDA release amount was reduced to 0.002 2 μmol/mg when adding the enzymatic hydrolysis oligosaccharides 10%).
Laminaria japonica was treated by biological enzymes degradation technology, and the effects of the L. japonica enzymatic hydrolysate on the growth and immune-related factors expression of sea cucumber was studied, and to explore the application prospect of the L. japonica enzymatic hydrolysate as a bait additive. The results showed that the L. japonica enzymatic hydrolysate including oligosaccharides and mud could promote the growth of sea cucumber. The effect of adding oligosaccharide 10% and mud 25% was the optimal, and the growth rate of sea cucumber was 76.60%and 40.30%, respectively. In addition, the enzymatic hydrolysis oligosaccharides of L. japonica could increase the activity of immune-related factors including superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), alkaline phosphatase(ACP), acid phosphatase(AKP) in the perisome tissue of sea cucumber, and decrease the production of malondialdehyde(MDA), which could protect the body from oxidative damage(MDA release amount was reduced to 0.002 2 μmol/mg when adding the enzymatic hydrolysis oligosaccharides 10%).
引文
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[26]谭雪丽,鲁梅,李美麒,等.重金属胁迫下2株芽孢杆菌的形成及生物学特征[J].贵州农业科学,2018,46(2):74-77.
[27]陈仲,蒋经纬,高杉,等.不同病原菌刺激后仿刺参幼参体腔细胞中免疫相关酶的应答变化[J].水产科学,2018,37(3):295-300.
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[30]曹新芳,黄卉卉,任秋楠,等.泥鳅消化道过氧化物酶、三磷酸腺苷酶、琥珀酸脱氢酶、酸性磷酸酶、碱性磷酸酶及非特异性酯酶的分布与组织定位[J].中国组织化学与细胞化学杂志,2018,27(5):453-458.
[31]FERN魣NDEZ J,PEREZ-ALVAREZ J A,FERNANDEZ-LOPEZ J A.Thiobarbituric acid test for monitoring lipid oxidation in meat[J].Food Chem,1997,59(3):345-353.
[2]赵玲,马红伟,曹荣,等.10种海参营养成分分析[J].食品安全质量检测学报,2016,7(7):2867-2872.
[3]ROGGATZ C C,GONZALEZ-WANGUEMERT M,PEREIRCA H,et al.A first glance into the nutritional properties of the sea cucumber Parastichopus regalis from the Mediterranean Sea(SE Spain)[J].Nat Prod Res,2018,32(1):116-120.
[4]刘哲.海参养殖中微生态制剂的应用探讨[J].现代农村科技,2018(8):45.
[5]XUE Z,LI H,WANG X L,et al.A review of the immune molecules in the sea cucumber[J].Fish Sheiifish Immun,2015,44(1):1-11.
[6]钟鸣,胡超群.海参养殖饲料学研究进展[J].饲料工业,2016,37(18):58-64.
[7]姚骏,张弘,郭森,等.海带的生物活性及系列产品开发研究进展[J].食品研究与开发,2018,39(8):198-202.
[8]王琪琳.海带硫酸多糖降解研究进展[J].聊城大学学报:自然科学版,2007,20(1):49-52.
[9]谢瑾,林宗毅,王智荣,等.海带多糖酶法降解及其产物生物活性的研究[J].食品研究与开发,2016,37(20):23-27.
[10]史德杰,孙远远,何冬晓,等.海参养殖技术探讨[J].河北渔业,2017(2):35-36.
[11]缪栋.海参养殖池微生物多样性及其动态研究[D].扬州:扬州大学,2017.
[12]陈效儒.对虾与海参高效免疫激活物质的筛选与评价[D].青岛:中国海洋大学,2009.
[13]李桂英.对虾肠道益生菌对凡纳滨对虾非特异免疫力和抗病力影响的研究[D].青岛:中国海洋大学,2011.
[14]赵聚萍.三种刺参混养模式的研究[D].烟台:烟台大学,2018.
[15]季晓彤,王玲,薛鹏,等.海参组织蛋白酶K的部分酶学性质及其对海参自溶的影响[J].中国农业大学学报,2017,22(9):75-80.
[16]范鑫.AtNEK6在棉花旱盐胁迫响应中的表达分析研究[D].北京:中国农业科学院,2018.
[17]王小虎.水稻类病变早衰基因LMES3和LMES4的克隆与功能研究[D].扬州:扬州大学,2018.
[18]王仁君,王建国,丁宁,等.多氯联苯(Aroclor 1242)胁迫下鲤鱼肝脏组织氧化应激[J].海洋与湖沼,2018,49(6):1286-1293.
[19]王鹏,江晓路,江艳华,等.褐藻低聚糖对提高大菱鲆免疫机能的作用[J].海洋科学,2006,30(8):6-9.
[20]赵吉伟,李小龙.野生及养殖茴鱼体内ACP、AKP和CAT活力的比较研究[J].水产学志,2011(24):12-15.
[21]唐婉琴,张江惠,袁伦强.水体锰暴露对草鱼碱性磷酸酶、酸性磷酸酶及代谢的影响[J].重庆师范大学学报:自然科学版,2018,35(3):69-74.
[22]田荟琳.猪血中过氧化氢酶的提取纯化及性质研究[D].天津:天津商业大学,2007.
[23]周强,曹春艳.血清过氧化氢酶的比色测定[J].哈尔滨医科大学学报,2001,35(6):473-474.
[24]李丹彤,谢广成,李洪福,等.裙带菜和萱藻凝集素对刺参组织主要免疫酶活性的影响[J].水产学报,2011,35(4):524-528.
[25]陈雁,王玉敏.Nrf2在肺纤维化治疗中作用的研究进展[J].中国老年学杂志,2018,38(3):1254-1256.
[26]谭雪丽,鲁梅,李美麒,等.重金属胁迫下2株芽孢杆菌的形成及生物学特征[J].贵州农业科学,2018,46(2):74-77.
[27]陈仲,蒋经纬,高杉,等.不同病原菌刺激后仿刺参幼参体腔细胞中免疫相关酶的应答变化[J].水产科学,2018,37(3):295-300.
[28]CHEN J,GAO G Z,FANG X M,et al.Effects of drought stress on activity of POD and CAT in wheat during generation period[J].J Anhui Agr Sci,2013,14(11):1529-1531.
[29]李强,姚霞,孙楷,等.不同光质对茅苍术生长、抗氧化酶活性及挥发油含量的影响[J].中国实验方剂杂志,2018,24(5):27-32.
[30]曹新芳,黄卉卉,任秋楠,等.泥鳅消化道过氧化物酶、三磷酸腺苷酶、琥珀酸脱氢酶、酸性磷酸酶、碱性磷酸酶及非特异性酯酶的分布与组织定位[J].中国组织化学与细胞化学杂志,2018,27(5):453-458.
[31]FERN魣NDEZ J,PEREZ-ALVAREZ J A,FERNANDEZ-LOPEZ J A.Thiobarbituric acid test for monitoring lipid oxidation in meat[J].Food Chem,1997,59(3):345-353.