秸秆生物酶法制备可发酵葡萄糖和木糖的分析研究
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摘要
目前秸秆纤维素酶解之前大部分都进行了预处理,提高秸秆水解率,预处理方法主要为物理或化学方法,需要化学试剂以及大量的能量。本实验直接使用纤维素酶、木聚糖酶处理秸秆纤维素,分别在最佳酶活条件下处理秸秆粉末。纤维素酶、木聚糖酶处理的秸秆水解率分别可达27.5%、15%。两种酶混合以后使用,在反应时间72h时达到最大的水解率为43.1%。
At present, most of the straw cellulose is pretreated before enzymatic hydrolysis to increase the hydrolysis rate of the straw. The pretreatment method is mainly physical or chemical methods, requiring chemical reagents and a large amount of energy consumption. In this experiment, cellulase and xylanase were directly used to treat straw cellulose, and the straw powder was treated under the optimal conditions of enzyme activity. The hydrolysis rates of cellulase and xylanase treated straws were respectively up to 27.5% and 15%. The two enzymes were mixed and used,the maximum hydrolysis rate reached 43.1% at the reaction time of 72 h.
At present, most of the straw cellulose is pretreated before enzymatic hydrolysis to increase the hydrolysis rate of the straw. The pretreatment method is mainly physical or chemical methods, requiring chemical reagents and a large amount of energy consumption. In this experiment, cellulase and xylanase were directly used to treat straw cellulose, and the straw powder was treated under the optimal conditions of enzyme activity. The hydrolysis rates of cellulase and xylanase treated straws were respectively up to 27.5% and 15%. The two enzymes were mixed and used,the maximum hydrolysis rate reached 43.1% at the reaction time of 72 h.
引文
[1]杨为华,李凤和.常压法秸秆预处理技术研究进展[J].化工进展.2017,36(3):873-880.
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[5]宋安东,王明道,任天保,等.秸秆双酶糖化条件试验研究[J].信阳师范学院学报.2006,19(2):181-184.
[6]石淑兰,何福望.制浆造纸分析与检测[M].北京:中国轻工业出版社,2012:35:-37;40-49.
[2]MENON V,RAO M.Trends in bioconversion of lignocellulose:Biofuels,platform chemicals&biorefinery concept[J].Progress in Energy and Combustion Science,2012,38(4):522-550.
[3]高奇瑞,夏金兰,单扬,等.超声波空化效应对纤维素酶降解秸秆影响的研究[J].山东农业大学学报(自然科学版).2014,45(2):237-242.
[4]张宇昊,王颉,张伟,等.半纤维素发酵生产燃料乙醇的研究进展[J].酿酒科技.2004,(5):72-74.
[5]宋安东,王明道,任天保,等.秸秆双酶糖化条件试验研究[J].信阳师范学院学报.2006,19(2):181-184.
[6]石淑兰,何福望.制浆造纸分析与检测[M].北京:中国轻工业出版社,2012:35:-37;40-49.