外周血中TREC水平与HIV感染疾病进展的关系
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摘要
目的建立T细胞受体脱氧核糖核酸重排删除环(TREC)的检测方法,并分析TREC与艾滋病疾病进展之间的关系。方法利用实时定量聚合酶链反应(PCR)技术,建立检测TREC水平的方法,并对未治疗病人及治疗后免疫重建成功与失败病人外周血中TREC水平进行分析。结果 85例未治疗的艾滋病病毒(HIV)感染者,CD4~+T淋巴细胞(简称CD4细胞)>350个/μL病人的TREC水平(32.5±7.65)高于CD4细胞在200～350个/μL的病人(19.9±6.26),而CD4细胞<200个/μL病人的TREC水平最低(9.5±1.87);免疫重建成功病人的TREC水平(28.2±10.1)远高于免疫重建失败病人(10±5.1)。结论未治疗的HIV感染者,随着CD4细胞水平的增加,外周血中TREC细胞的水平也明显增加;免疫重建成功病人外周血中TREC的水平明显高于免疫重建失败的病人。
Objective To establish a method for detection of TREC and to analyze the relationship between the levels of TREC in peripheral blood and the progression of HIV infection. Methods We established real-time PCR method to detect TREC levels in peripheral blood of untreated patients and patients with successful or failed immune reconstitution after treatment and compared the differences of TREC between different patients. Results In untreated patients with the increased CD4, TREC level was significantly increasing. The level of TREC was significantly higher in the successful immune reconstitution patients than that in the patients of immune reconstitution failure. Conclusion The level of TREC cells in peripheral blood increases significantly with the increase of CD4 cell level in untreated patients. The level of TREC in peripheral blood of successful immune reconstruction patients is significantly higher than that of patients with failed immune reconstruction.
Objective To establish a method for detection of TREC and to analyze the relationship between the levels of TREC in peripheral blood and the progression of HIV infection. Methods We established real-time PCR method to detect TREC levels in peripheral blood of untreated patients and patients with successful or failed immune reconstitution after treatment and compared the differences of TREC between different patients. Results In untreated patients with the increased CD4, TREC level was significantly increasing. The level of TREC was significantly higher in the successful immune reconstitution patients than that in the patients of immune reconstitution failure. Conclusion The level of TREC cells in peripheral blood increases significantly with the increase of CD4 cell level in untreated patients. The level of TREC in peripheral blood of successful immune reconstruction patients is significantly higher than that of patients with failed immune reconstruction.
引文
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[10] Sandgaard KS,Lewis J,Adams S,et al.Antiretroviral therapy increases thymic output in children with HIV[J].AIDS,2014(28):209-214.
[11] Zhang L,Lewin SR,Markowitz M,et al.Measuring recent thymic emigrants in blood of normal and HIV-1-infected individuals before and after effective therapy[J].J Exp Med,1999(190):725-732.
[12] Blanche S,Scott-Algara D,Le Chenadec J,et al.na?ve T lymphocytes and recent thymic emigrants are associated with HIV-1 disease history in french adolescents and young adults infected in the perinatal period:the ANRS-EP38-IMMIP study[J].Clin Infect Dis,2014(58):573-587.
[2] 田云飞,肖江,韩宁,等.HIV/AIDS病人免疫重建不良的研究进展[J].中华实验和临床感染病杂志,2015,9(4):462-467.
[3] Cao W,Mehraj V,Trottier B.Early Initiation Rather Than Prolonged Duration of Antiretroviral Therapy in HIV Infection Contributes to the Normalization of CD8 T-Cell Counts[J].Clin Infect Dis,2016(62):250-257.
[4] 施万英.中国HIV/AIDS病人naive、memory CD8+T细胞亚群数量及功能与疾病进展的相关性研究[D].沈阳:中国医科大学,2017.
[5] Sandgaard KS,Lewis J,Adams S,et al.Antiretroviral therapy increases thymic output in children with HIV[J].AIDS,2014(28):209-214.
[6] Warszawski J,Thomas C,Dialla O,et al.T-Cell Receptor Excision Circles in HIV-Exposed,Uninfected Newborns Measured During a National Newborn Screening Program for Severe Combined Immunodeficiency[J].J Pediatr,2018(202):311-314.
[7] Miyamoto M,Gouvêa AFTB,Ono E,et al.Immune development in HIV-exposed uninfected children born to HIV-infected women[J].Rev Inst Med Trop Sao Paulo,2017(59):e30.
[8] Ferrando-Martinez S,De Pablo-Bernal RS,De Luna-Romero M,et al.Thymic Function Failure Is Associated With Human Immunodeficiency Virus Disease Progression[J].Clin Infect Dis,2017(64):1191-1197.
[9] Drylewicz J,Vrisekoop N,Mugwagwa T,et al.Reconciling Longitudinal Naive T-Cell and TREC Dynamics during HIV-1 Infection[J].PLoS One,2016(11):e0152513.
[10] Sandgaard KS,Lewis J,Adams S,et al.Antiretroviral therapy increases thymic output in children with HIV[J].AIDS,2014(28):209-214.
[11] Zhang L,Lewin SR,Markowitz M,et al.Measuring recent thymic emigrants in blood of normal and HIV-1-infected individuals before and after effective therapy[J].J Exp Med,1999(190):725-732.
[12] Blanche S,Scott-Algara D,Le Chenadec J,et al.na?ve T lymphocytes and recent thymic emigrants are associated with HIV-1 disease history in french adolescents and young adults infected in the perinatal period:the ANRS-EP38-IMMIP study[J].Clin Infect Dis,2014(58):573-587.