不同基因型烟草腺毛主要分泌物差异分析
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摘要
为明确不同基因型烟草腺毛分泌物的成分差异,筛选高腺毛分泌物基因型的烟草种质,本研究基于气相色谱-质谱联用仪(GC/MS),对不同基因型的12个烟草品种的鲜烟叶腺毛主要分泌物进行鉴定分析,结果表明:在12份检测品种烟叶中,西柏三烯二醇(CBT-diols)是西柏烷二萜的主要物质形式,且其在烤烟品种中含量相对较高;所有检测品种中均含有α-西柏三烯二醇(α-CBT-diols)和β-西柏三烯二醇(β-CBT-diols),两者比值变化范围为1.58~2.32;所有检测品种中共检测出5个蔗糖酯类型,可依据蔗糖酯类型的有无和含量的多少将检测种质分为Ⅰ-Ⅱ型蔗糖酯较多和Ⅲ-V型蔗糖酯较多2个大类。总体上看,Basma、Beinhart1000-1、红花大金元、BX1、BX2这5个品种腺毛主要分泌物含量较高,可供烟草香气品质改良育种或表面活性物质提取利用。
In order to clarify differences of glandular trichome secretions between different tobacco genotypes and to screen tobacco varieties with higher trichome secretions, 12 tobacco varieties were identified by gas chromatography-mass spectrometry(GC/MS) in this study. Results showed that cembratrien-diols(CBT-diols) were the main cembranoids among tested varieties, and the content of CBTdiols of flue-cured tobacco was relatively higher. All tested varieties contain α-and β-cembratrien-diols, and the content ratio of α-to β-cembratrien-diols was 1.58~2.32. Five types of sucrose esters were detected, and the tested varieties were divided into two groups according to quality and quantity of sucrose esters: varieties of group one contain higher Ⅰ-Ⅱ and varieties of group two contain higher Ⅲ-V types of sucrose esters. In general, Basma, Beinhart1000-1, Honghuadajinyuan, BX1 and BX2 have more glandular secretions and can be further used in tobacco breeding and extraction of tobacco leaf surfactants.
In order to clarify differences of glandular trichome secretions between different tobacco genotypes and to screen tobacco varieties with higher trichome secretions, 12 tobacco varieties were identified by gas chromatography-mass spectrometry(GC/MS) in this study. Results showed that cembratrien-diols(CBT-diols) were the main cembranoids among tested varieties, and the content of CBTdiols of flue-cured tobacco was relatively higher. All tested varieties contain α-and β-cembratrien-diols, and the content ratio of α-to β-cembratrien-diols was 1.58~2.32. Five types of sucrose esters were detected, and the tested varieties were divided into two groups according to quality and quantity of sucrose esters: varieties of group one contain higher Ⅰ-Ⅱ and varieties of group two contain higher Ⅲ-V types of sucrose esters. In general, Basma, Beinhart1000-1, Honghuadajinyuan, BX1 and BX2 have more glandular secretions and can be further used in tobacco breeding and extraction of tobacco leaf surfactants.
引文
[1]李霞,蔡恒,杨超,等.烟叶腺毛研究进展[J].中国农学通报,2008,24(4):261-264.LI Xia,CAI Heng,YANG Chao,et al.Advances in research on tobacco glandular hair[J].Chinese Agricultural Science Bulletin,2008,4(4):261-264
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[3]LIN Y,WAGNER G J.Surface disposition and stability of pestinteractive,trichome-exuded diterpenes and sucrose esters of tobacco[J].J Chem Ecol,1994,20(8):1907-1921.
[4]Severson R F,Johnson A W,Jackson D M.Cuticular constituents of tobacco:Factors affecting their production and their role in insect and disease resistance and smoke quality[C]//Tobacco Chemists'Research Conference.1985.
[5]ROBERTS D L.Natural tobacco flavor[J].Recent CBT-diolsobsci,1988.
[6]向章敏,周淑平,耿召良.烟叶表面化学成分的分析研究进展[J].西南农业学报,2015,28(3):1395-1398.XIANG Zhangmin,ZHOU Shuping,GENG Shaoliang.Research progress of chemical compositions on tobacco leaves[J].Southwest China Journal of Agricultural Sciences,2015,28(3):1395-1398.
[7]Lange B M,Croteau R.Isoprenoid biosynthesis via a mevalonateindependent pathway in plants:cloning and heterologous expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from peppermint[J].Archives of Biochemistry&Biophysics,1999,365(1):170-174.
[8]李伟.烟草叶面腺毛密度和分泌物量的变异与遗传特性[D];郑州:河南农业大学,2004.LI Wei.Variation and genetic characteristics of leaf gland density and secretion in tobacco leaves[D].Henan Agricultural University,2004.
[9]刘兴祥.解析我国烟草香精香料的研究与应用[J].中国农业信息,2014,(7):247.LIU Xingxiang.Research and application of tobacco flavors and fragrances in China[J].China Agricultural Information,2014,(7):247.
[10]叶荣飞,赵瑞峰.我国烟草香精香料的研究现状[J].清远职业技术学院学报,2011,04(3):34-37.YE Rongfei,ZHAO Ruifeng.Research status of tobacco flavors and fragrances in China[J].Journal of Qingyuan Vocational and Technical College,2011,04(3):34-37.
[11]余金恒.许明忠,黄锋林,等.烟用香精香料物质研究进展[J].河南农业科学,2011,40(2):16-18,24.YU Jinheng,XU Mingzhong,HUANG Fenglin,et al.Advances in materials research of tobacco flavors and fragrances[J],Journal of Henan Agricultural Sciences,2011,40(2):16-18,24.
[12]HAWTHORNE D J,SHAPIRO J A,TINGEY W M,et al.Trichome-borne and artificially applied acylsugars of wild tomato deter feeding and oviposition of the leafminer Liriomyza trifolii[J].Entomologia Experimentalis Et Applicata,1992,65(1):65-73.
[13]RODRIGUEZ AE,TINGEY WM,MUTSCHLER MA.Acylsugars of lycopersicon pennellii deter settling and feeding of the green peach aphid(Homoptera:Aphididae)[J].Journal of Economic Entomology,1993,86(86):34-39.
[14]LIEDL B E,LAWSON D M,WHITE K K,et al.Acylsugars of wild tomato lycopersicon pennellii alters settling and reduces oviposition of bemisia argentifolii(Homoptera:Aleyrodidae)[J].Journal of Economic Entomology,1995,88(3):742-748.
[15]ALBA J M,MONTSERRAT M,FERN NDEZMU OZ R.Resistance to the two-spotted spider mite(Tetranychus urticae)by acylsucroses of wild tomato(Solanum pimpinellifolium)trichomes studied in a recombinant inbred line population[J].Experimental and Applied Acarology,2009,47(1):35-47.
[16]HILL K,RHODE O.Sugar-based surfactants for consumer products and technical applications[J].Fett/lipid,1999,101(1):25-33.
[17]冯琦.烟草腺毛发育及类萜代谢研究[D].河南农业大学,2013.FENG Qi,Study on Glandular Hair Growth and Terpene Metabolism of Tobacco[D].Henan Agricultural University,2013.
[18]SCHILMILLER A L,MOGHE GD,FAN P,et al.Functionally divergent alleles and duplicated Loci encoding an acyltransferase contribute to acylsugar metabolite diversity in Solanum trichomes[J].The Plant cell,2015,27(4):1002-1007.
[19]SCHILMILLER A L,LAST R L,PICHERSKYE.Harnessing plant trichome biochemistry for the production of useful compounds[J].Plant Journal,2008,54(4):702-711.
[20]SCHILMILLER A L,CHARBONNEAU A L,LAST R L.Identification of a BAHD acetyltransferase that produces protective acyl sugars in tomato trichomes[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(40):16377-16382.
[21]SCHILMILLER AL,MINER DP,LARSON M,et al.Studies of a biochemical factory:tomato trichome deep expressed sequence tag sequencing and proteomics[J].Plant physiology,2010,153(3):1212-1223.
[22]任夏,燕志强,金辉,β-西柏三烯二醇对拟南芥的化感效应及其作用机理研究[J].西北植物学报,2017,37(4):713-718.REN Xia,YAN Zhiqiang,JIN Hui,Study on the allelopathic effect ofβ-cyproterone on Arabidopsis thaliana and its mechanism[J].Journal of Northwest Botanical Sciences,2017,37(4):713-718.
[23]段苏珍,杜咏梅,侯小东,等.烟草提取物对十一种植物病原真菌的抑制作用[J].天然产物研究与开发,2015,27(3):470-474,484.DUAN Suzhen,DU Yongmei,HOU Xiaodong,et al.Inhibitory effects of tobacco extract on eleven phytopathogenic fungi[J].Research and Development of Natural Products,2015,27(3):470-474,484.
[24]蔡莉莉,谢复炜,刘克建,等.香料烟中蔗糖酯的气相色谱/质谱分析[J].烟草科技,2009,(3):40-44.CAI Lili,XIE Fuwei,LIU Kejian,et al.Analysis of sucrose ester in oriental tobacco by gas chromatography/mass spectrometry[J].Tobacco Science&Technology,2009,(3):40-44.
[25]Severson R F,Arrendale R F,Chortyk O T,et al.Isolation and characterization of the sucrose esters of the cuticular waxes of green tobacco leaf.[J].Journal of Agricultural&Food Chemistry,1985,33(5):870-875.
[26]VONTIMITTA V,DANEHOWER DA,STEEDE T,et al.Analysis of a Nicotiana tabacum L.genomic region controlling two leaf surface chemistry traits[J].Journal of Agricultural&Food Chemistry,2010,58(1):294-300.
[27]WANG E,WANG R,DEPARASIS J,et al.Suppression of a P450hydroxylase gene in plant trichome glands enhances naturalproduct-based aphid resistance[J].Nature biotechnology,2001,19(4):371-374.
[28]吴云平,潘文杰,李章海,等.不同品种烤烟鲜叶表面提取物主要成分比较[J].中国农学通报,2011,27(2):353-359.WU Yunping,PAN Wenjie,LI Zhanghai,et al.Comparison of Major Cuticular Components from Cured Leaves of Different Fluecured Tobacco Varieties[J].Chinese Agricultural Science Bulletin,2011,27(2):353-359.
[29]王冬,张小全,杨铁钊,等.类西柏烷二萜代谢机理及调控研究进展[J].中国烟草学报,2014,20(3):113-118.WANG Dong,ZHANG Xiaoquan,YANG Tiezhao,et al.Advances in metabolic mechanism and regulation of cetyl-like diterpenoids[J].Chinese Journal of Tobacco,2014,20(3):113-118.
[30]何峰,西柏三烯-4,6-二醇的分离纯化及热裂解和烟草中HPLC分析方法研究[D].郑州轻工业学院,2015.HE Feng,Separation,Purification and Pyrolysis of Ciprotermine-4,6-Diol and HPLC Analytical Methods in Tobacco[D].Zhengzhou Institute of Light Industry,2015.
[31]SALLAUD C,GIACALONE C,TPFER R,et al.Characterization of two genes for the biosynthesis of the labdane diterpene Z-abienol in tobacco(Nicotiana tabacum)glandular trichomes[J].Plant Journal,2013,74(4):1-17.
[32]王伟,孔光辉,李佛琳,等.烤烟烟叶腺毛及其分泌物研究进展[J].中国农学通报,2007,23(2):251-254.WANG Wei,KONG Guanghui,LI Folin,et al.Research progress on glandular hair and its secretions in flue-cured tobacco leaves[J].Chinese Agricultural Science Bulletin,2007,23(2):251-254.
[2]戴维斯,尼尔森编.烟草-生产,化学和技术[M].化学工业出版社,2003.DAVIS D L,NIELSEN M.Tobacco-Production,Chemistry and Technology[M].Chemical Industry Press,2003.
[3]LIN Y,WAGNER G J.Surface disposition and stability of pestinteractive,trichome-exuded diterpenes and sucrose esters of tobacco[J].J Chem Ecol,1994,20(8):1907-1921.
[4]Severson R F,Johnson A W,Jackson D M.Cuticular constituents of tobacco:Factors affecting their production and their role in insect and disease resistance and smoke quality[C]//Tobacco Chemists'Research Conference.1985.
[5]ROBERTS D L.Natural tobacco flavor[J].Recent CBT-diolsobsci,1988.
[6]向章敏,周淑平,耿召良.烟叶表面化学成分的分析研究进展[J].西南农业学报,2015,28(3):1395-1398.XIANG Zhangmin,ZHOU Shuping,GENG Shaoliang.Research progress of chemical compositions on tobacco leaves[J].Southwest China Journal of Agricultural Sciences,2015,28(3):1395-1398.
[7]Lange B M,Croteau R.Isoprenoid biosynthesis via a mevalonateindependent pathway in plants:cloning and heterologous expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from peppermint[J].Archives of Biochemistry&Biophysics,1999,365(1):170-174.
[8]李伟.烟草叶面腺毛密度和分泌物量的变异与遗传特性[D];郑州:河南农业大学,2004.LI Wei.Variation and genetic characteristics of leaf gland density and secretion in tobacco leaves[D].Henan Agricultural University,2004.
[9]刘兴祥.解析我国烟草香精香料的研究与应用[J].中国农业信息,2014,(7):247.LIU Xingxiang.Research and application of tobacco flavors and fragrances in China[J].China Agricultural Information,2014,(7):247.
[10]叶荣飞,赵瑞峰.我国烟草香精香料的研究现状[J].清远职业技术学院学报,2011,04(3):34-37.YE Rongfei,ZHAO Ruifeng.Research status of tobacco flavors and fragrances in China[J].Journal of Qingyuan Vocational and Technical College,2011,04(3):34-37.
[11]余金恒.许明忠,黄锋林,等.烟用香精香料物质研究进展[J].河南农业科学,2011,40(2):16-18,24.YU Jinheng,XU Mingzhong,HUANG Fenglin,et al.Advances in materials research of tobacco flavors and fragrances[J],Journal of Henan Agricultural Sciences,2011,40(2):16-18,24.
[12]HAWTHORNE D J,SHAPIRO J A,TINGEY W M,et al.Trichome-borne and artificially applied acylsugars of wild tomato deter feeding and oviposition of the leafminer Liriomyza trifolii[J].Entomologia Experimentalis Et Applicata,1992,65(1):65-73.
[13]RODRIGUEZ AE,TINGEY WM,MUTSCHLER MA.Acylsugars of lycopersicon pennellii deter settling and feeding of the green peach aphid(Homoptera:Aphididae)[J].Journal of Economic Entomology,1993,86(86):34-39.
[14]LIEDL B E,LAWSON D M,WHITE K K,et al.Acylsugars of wild tomato lycopersicon pennellii alters settling and reduces oviposition of bemisia argentifolii(Homoptera:Aleyrodidae)[J].Journal of Economic Entomology,1995,88(3):742-748.
[15]ALBA J M,MONTSERRAT M,FERN NDEZMU OZ R.Resistance to the two-spotted spider mite(Tetranychus urticae)by acylsucroses of wild tomato(Solanum pimpinellifolium)trichomes studied in a recombinant inbred line population[J].Experimental and Applied Acarology,2009,47(1):35-47.
[16]HILL K,RHODE O.Sugar-based surfactants for consumer products and technical applications[J].Fett/lipid,1999,101(1):25-33.
[17]冯琦.烟草腺毛发育及类萜代谢研究[D].河南农业大学,2013.FENG Qi,Study on Glandular Hair Growth and Terpene Metabolism of Tobacco[D].Henan Agricultural University,2013.
[18]SCHILMILLER A L,MOGHE GD,FAN P,et al.Functionally divergent alleles and duplicated Loci encoding an acyltransferase contribute to acylsugar metabolite diversity in Solanum trichomes[J].The Plant cell,2015,27(4):1002-1007.
[19]SCHILMILLER A L,LAST R L,PICHERSKYE.Harnessing plant trichome biochemistry for the production of useful compounds[J].Plant Journal,2008,54(4):702-711.
[20]SCHILMILLER A L,CHARBONNEAU A L,LAST R L.Identification of a BAHD acetyltransferase that produces protective acyl sugars in tomato trichomes[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(40):16377-16382.
[21]SCHILMILLER AL,MINER DP,LARSON M,et al.Studies of a biochemical factory:tomato trichome deep expressed sequence tag sequencing and proteomics[J].Plant physiology,2010,153(3):1212-1223.
[22]任夏,燕志强,金辉,β-西柏三烯二醇对拟南芥的化感效应及其作用机理研究[J].西北植物学报,2017,37(4):713-718.REN Xia,YAN Zhiqiang,JIN Hui,Study on the allelopathic effect ofβ-cyproterone on Arabidopsis thaliana and its mechanism[J].Journal of Northwest Botanical Sciences,2017,37(4):713-718.
[23]段苏珍,杜咏梅,侯小东,等.烟草提取物对十一种植物病原真菌的抑制作用[J].天然产物研究与开发,2015,27(3):470-474,484.DUAN Suzhen,DU Yongmei,HOU Xiaodong,et al.Inhibitory effects of tobacco extract on eleven phytopathogenic fungi[J].Research and Development of Natural Products,2015,27(3):470-474,484.
[24]蔡莉莉,谢复炜,刘克建,等.香料烟中蔗糖酯的气相色谱/质谱分析[J].烟草科技,2009,(3):40-44.CAI Lili,XIE Fuwei,LIU Kejian,et al.Analysis of sucrose ester in oriental tobacco by gas chromatography/mass spectrometry[J].Tobacco Science&Technology,2009,(3):40-44.
[25]Severson R F,Arrendale R F,Chortyk O T,et al.Isolation and characterization of the sucrose esters of the cuticular waxes of green tobacco leaf.[J].Journal of Agricultural&Food Chemistry,1985,33(5):870-875.
[26]VONTIMITTA V,DANEHOWER DA,STEEDE T,et al.Analysis of a Nicotiana tabacum L.genomic region controlling two leaf surface chemistry traits[J].Journal of Agricultural&Food Chemistry,2010,58(1):294-300.
[27]WANG E,WANG R,DEPARASIS J,et al.Suppression of a P450hydroxylase gene in plant trichome glands enhances naturalproduct-based aphid resistance[J].Nature biotechnology,2001,19(4):371-374.
[28]吴云平,潘文杰,李章海,等.不同品种烤烟鲜叶表面提取物主要成分比较[J].中国农学通报,2011,27(2):353-359.WU Yunping,PAN Wenjie,LI Zhanghai,et al.Comparison of Major Cuticular Components from Cured Leaves of Different Fluecured Tobacco Varieties[J].Chinese Agricultural Science Bulletin,2011,27(2):353-359.
[29]王冬,张小全,杨铁钊,等.类西柏烷二萜代谢机理及调控研究进展[J].中国烟草学报,2014,20(3):113-118.WANG Dong,ZHANG Xiaoquan,YANG Tiezhao,et al.Advances in metabolic mechanism and regulation of cetyl-like diterpenoids[J].Chinese Journal of Tobacco,2014,20(3):113-118.
[30]何峰,西柏三烯-4,6-二醇的分离纯化及热裂解和烟草中HPLC分析方法研究[D].郑州轻工业学院,2015.HE Feng,Separation,Purification and Pyrolysis of Ciprotermine-4,6-Diol and HPLC Analytical Methods in Tobacco[D].Zhengzhou Institute of Light Industry,2015.
[31]SALLAUD C,GIACALONE C,TPFER R,et al.Characterization of two genes for the biosynthesis of the labdane diterpene Z-abienol in tobacco(Nicotiana tabacum)glandular trichomes[J].Plant Journal,2013,74(4):1-17.
[32]王伟,孔光辉,李佛琳,等.烤烟烟叶腺毛及其分泌物研究进展[J].中国农学通报,2007,23(2):251-254.WANG Wei,KONG Guanghui,LI Folin,et al.Research progress on glandular hair and its secretions in flue-cured tobacco leaves[J].Chinese Agricultural Science Bulletin,2007,23(2):251-254.