丁香酚对黄斑篮子鱼幼鱼麻醉效果的研究
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摘要
该研究在水温为(29.3±1.1)℃的自然海水条件下,测定了不同质量浓度丁香酚对体质量为(4.83±1.30)g的黄斑篮子鱼(Siganus oramin)的麻醉效果。结果显示,在30～80 mg·L–1时,黄斑篮子鱼幼鱼的入麻时间随其浓度的升高而减少,复苏时间随其浓度的升高而增加。麻醉过程中,呼吸频率在浅麻时略微上升,深麻时快速下降,直至麻醉过度呼吸停止。研究发现,丁香酚质量浓度在30～100 mg·L~(–1)时,黄斑篮子鱼幼鱼均能在3 min内进入4期麻醉,4 min内完全复苏,成活率100%。入麻后在空气中暴露时间少于8 min时,复苏时间随暴露时间的增加而减少,大于8 min时,复苏时间随暴露时间的增加而延长,且复苏率逐渐下降。结果表明,丁香酚对黄斑篮子鱼幼鱼具有入麻快、复苏时间短、复苏率高等优点,是其理想的麻醉剂;建议生产上使用的质量浓度为30 mg·L~(–1),且入麻后人工操作不宜超过6 min,若追求更快麻醉,丁香酚质量浓度也不宜超过80 mg·L~(–1)。
We investigated the anaesthetic effect of eugenol at different mass concentrations on juvenile Siganus oramin with body mass of(4.83±1.30)g at natural seawater at temperature of(29.3±1.1)℃.The results show that when the mass concentration of eugenol was 30-80 mg·L~(–1),the anesthesia time reduced but the recovery time increased with the increase of mass concentration of eugenol.During the anesthesia,the respiratory rate of juvenile S.oramin increased at light anesthesia state,but decreased rapidly during deep anesthesia until the respiratory arrested due to excessive anesthesia.When the mass concentration of eugenol was 30-100mg·L~(–1),the juvenile S.oramin could reach Stage 4 anesthesia within 3 min and recovered less than 4 min with a survival rate of100%.When the air exposure time was within 8 min,the recovery time slightly reduced with extension of air exposure time;when the air exposure time was longer than 8 min,the recovery time increased but the recovery rate decreased with the extension of air exposure time.It is indicated that eugenol,with advantages of fast anesthesia,short recovery time and high recovery rate,is an ideal anesthetic for juvenile S.oramin.The concentration of eugenol is suggested to be 30 mg·L~(–1) in production,and the artificial operation time should not exceed 6 min after anesthesia.The anesthesia concentration should not exceed 80 mg·L~(–1) even for faster anesthesia.
We investigated the anaesthetic effect of eugenol at different mass concentrations on juvenile Siganus oramin with body mass of(4.83±1.30)g at natural seawater at temperature of(29.3±1.1)℃.The results show that when the mass concentration of eugenol was 30-80 mg·L~(–1),the anesthesia time reduced but the recovery time increased with the increase of mass concentration of eugenol.During the anesthesia,the respiratory rate of juvenile S.oramin increased at light anesthesia state,but decreased rapidly during deep anesthesia until the respiratory arrested due to excessive anesthesia.When the mass concentration of eugenol was 30-100mg·L~(–1),the juvenile S.oramin could reach Stage 4 anesthesia within 3 min and recovered less than 4 min with a survival rate of100%.When the air exposure time was within 8 min,the recovery time slightly reduced with extension of air exposure time;when the air exposure time was longer than 8 min,the recovery time increased but the recovery rate decreased with the extension of air exposure time.It is indicated that eugenol,with advantages of fast anesthesia,short recovery time and high recovery rate,is an ideal anesthetic for juvenile S.oramin.The concentration of eugenol is suggested to be 30 mg·L~(–1) in production,and the artificial operation time should not exceed 6 min after anesthesia.The anesthesia concentration should not exceed 80 mg·L~(–1) even for faster anesthesia.
引文
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[12]苏明明,孙兴权,杨春光,等.渔用麻醉剂MS-222、丁香酚在鲜活水产品运输中的应用及检测方法研究进展[J].食品安全质量检测学报,2015(1):25-29.
[13]金元,何雅静,程波,等.丁香酚类麻醉剂的安全性探讨[J].食品安全质量检测学报,2017,8(1):33-40.
[14]刘春花,赵长臣,巩华,等.丁香油麻醉剂在水产养殖上的应用研究进展[J].广东饲料,2016,25(11):33-36.
[15]王正全,崔云云,倪玲,等.运输暂养水体中丁香酚残留检测研究进展[J].中国渔业质量与标准,2018(2):57-65.
[16]匡刚桥,李萍,郑曙明,等.丁香酚对斑点叉尾鮰幼鱼的麻醉效果[J].四川动物,2010,29(5):584-587.
[17]陈德芳,汪开毓,廖国平,等.丁香酚对斑马鱼麻醉效果的研究[J].水产科技情报,2013,40(4):193-198.
[18]李靖,刘宝良,王顺奎,等.丁香酚对大西洋鲑麻醉效果的实验研究[J].海洋科学进展,2015,33(1):92-99.
[19]杨移斌,夏永涛,艾晓辉,等.丁香酚对俄罗斯鲟的麻醉效果[J].中国渔业质量与标准,2016,6(1):17-21.
[20]陈德芳,汪开毓,肖丹,等.丁香酚对鲫鱼麻醉效果的研究[J].水产科技情报,2010,37(6):288-291.
[21]ZHANG F L,LU S M,HAN J W.A study on the anesthetic effect of Eugenol to Cyprinus carpio Linnaeus[J].J.Mount Agr Biol,2007.
[22]罗晶晶,韩典峰,孙玉增,等.丁香酚对罗非鱼的麻醉效果[J].河北渔业,2018(3):5-8,42.
[23]张志山,朱树人,安丽,等.丁香酚对翘嘴鲌的麻醉效果研究[J].长江大学学报(自然科学版),2016,13(27):39-43.
[24]PATTANASIRI T,TAPARHUDEE W,SUPPAKUL P.Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish,Betta splendens[J].Aquacult Int,2017,25(1):163-175.
[25]TARKHANI R,IMANI A,JAMALI H,et al.Anaesthetic efficacy of eugenol on various size classes of angelfish(Pterophyllum scalare Schultze,1823)[J].Aquac Res,2017,48(10):5263-5270.
[26]TARKHANI R,IMANI A,JAMALI H,et al.Anaesthetic efficacy of eugenol on Flowerhorn(Amphilophus labiatus×Amphilophus trimaculatus)[J].Aquacult Res,2017,48(6):3207-3215.
[27]胡发文,李莉,刘元文,等.丁香油和MS-222对大泷六线鱼幼鱼的麻醉效果[J].大连海洋大学学报,2017,32(6):671-675.
[28]彭宁东,汤文圣,郭栋,等.MS-222对赤眼鳟幼鱼麻醉效果的研究[J].华南师范大学学报(自然科学版),2016,48(6):37-43.
[29]俞懿强,邹德荣.丁香酚的药理作用及在口腔医学应用的研究进展[J].临床口腔医学杂志,2009,25(3):187-189.
[2]赵峰,章龙珍,庄平,等.长鳍篮子鱼的外形特征与消化系统结构[J].海洋渔业,2008,30(3):202-206.
[3]黄小林,李涛,林黑着,等.网箱养殖黄斑篮子鱼胚胎发育观察[J].南方水产科学,2018,14(2):96-101.
[4]庄平,宋超,章龙珍,等.黄斑篮子鱼肌肉营养成分与品质的评价[J].水产学报,2008,32(1):77-83.
[5]吕旭宁,蒋增杰,方建光,等.黄斑篮子鱼(Siganus oramin)对北方养殖网箱网衣附着藻类的生物清除作用[J].渔业科学进展,2017,38(5):50-56.
[6]冯广朋,章龙珍,庄平,等.海水网箱养殖长鳍篮子鱼的摄食与生长特性[J].海洋渔业,2008,30(1):37-42.
[7]梁前才,车南青.长鳍篮子鱼池塘养殖技术[J].中国水产,2018(2):83-84.
[8]林川,赵爽,黄海,等.点篮子鱼深海网箱养殖试验[J].水产养殖,2017,38(12):4-6.
[9]方卫东.褐篮子鱼网箱养殖技术[J].现代农业科技,2012(13):278-279.
[10]刘长琳,何力,陈四清,等.鱼类麻醉研究综述[J].渔业现代化,2007,34(5):21-25.
[11]刘骁,谢晶,黄硕琳.鱼类保活运输的研究进展[J].食品与发酵工业,2015,41(8):255-260.
[12]苏明明,孙兴权,杨春光,等.渔用麻醉剂MS-222、丁香酚在鲜活水产品运输中的应用及检测方法研究进展[J].食品安全质量检测学报,2015(1):25-29.
[13]金元,何雅静,程波,等.丁香酚类麻醉剂的安全性探讨[J].食品安全质量检测学报,2017,8(1):33-40.
[14]刘春花,赵长臣,巩华,等.丁香油麻醉剂在水产养殖上的应用研究进展[J].广东饲料,2016,25(11):33-36.
[15]王正全,崔云云,倪玲,等.运输暂养水体中丁香酚残留检测研究进展[J].中国渔业质量与标准,2018(2):57-65.
[16]匡刚桥,李萍,郑曙明,等.丁香酚对斑点叉尾鮰幼鱼的麻醉效果[J].四川动物,2010,29(5):584-587.
[17]陈德芳,汪开毓,廖国平,等.丁香酚对斑马鱼麻醉效果的研究[J].水产科技情报,2013,40(4):193-198.
[18]李靖,刘宝良,王顺奎,等.丁香酚对大西洋鲑麻醉效果的实验研究[J].海洋科学进展,2015,33(1):92-99.
[19]杨移斌,夏永涛,艾晓辉,等.丁香酚对俄罗斯鲟的麻醉效果[J].中国渔业质量与标准,2016,6(1):17-21.
[20]陈德芳,汪开毓,肖丹,等.丁香酚对鲫鱼麻醉效果的研究[J].水产科技情报,2010,37(6):288-291.
[21]ZHANG F L,LU S M,HAN J W.A study on the anesthetic effect of Eugenol to Cyprinus carpio Linnaeus[J].J.Mount Agr Biol,2007.
[22]罗晶晶,韩典峰,孙玉增,等.丁香酚对罗非鱼的麻醉效果[J].河北渔业,2018(3):5-8,42.
[23]张志山,朱树人,安丽,等.丁香酚对翘嘴鲌的麻醉效果研究[J].长江大学学报(自然科学版),2016,13(27):39-43.
[24]PATTANASIRI T,TAPARHUDEE W,SUPPAKUL P.Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish,Betta splendens[J].Aquacult Int,2017,25(1):163-175.
[25]TARKHANI R,IMANI A,JAMALI H,et al.Anaesthetic efficacy of eugenol on various size classes of angelfish(Pterophyllum scalare Schultze,1823)[J].Aquac Res,2017,48(10):5263-5270.
[26]TARKHANI R,IMANI A,JAMALI H,et al.Anaesthetic efficacy of eugenol on Flowerhorn(Amphilophus labiatus×Amphilophus trimaculatus)[J].Aquacult Res,2017,48(6):3207-3215.
[27]胡发文,李莉,刘元文,等.丁香油和MS-222对大泷六线鱼幼鱼的麻醉效果[J].大连海洋大学学报,2017,32(6):671-675.
[28]彭宁东,汤文圣,郭栋,等.MS-222对赤眼鳟幼鱼麻醉效果的研究[J].华南师范大学学报(自然科学版),2016,48(6):37-43.
[29]俞懿强,邹德荣.丁香酚的药理作用及在口腔医学应用的研究进展[J].临床口腔医学杂志,2009,25(3):187-189.