水稻耐盐基因SKC1等位变异突变体耐盐性评价
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摘要
SKC1基因是已经克隆的水稻耐盐主效基因,位于水稻1号染色体。为了在水稻育种中高效利用SKC1耐盐基因,前期研究利用TILLING技术检测宁夏水稻主栽品种宁粳28号EMS诱变群体,得到了SKC1基因的SNP突变体。本研究通过芽期、苗期、孕穗期、全生育期耐盐性鉴定,对16份农艺性状优良的水稻耐盐基因SKC1等位变异突变体的耐盐性进行了全面评价,结果表明:耐盐性强的突变材料有:18 NY-10、18 NY-12、18 NY-14,耐盐性较强的突变材料有:18 NY-2、18 NY-4、18 NY-5、18 NY-6、18 NY-7、18 NY-8、18 NY-13、18 NY-16。结果可为宁夏水稻耐盐育种提供种质资源。
SKC1 gene is a major salt tolerance gene cloned from rice,which is located in chromosome 1 of rice.For efficient use of SKC1salt-tolerant genes in rice breeding,and previous studies using TILLING technology to detect EMS mutant population of Ningxia main rice variety Ningjing 28,and obtained SNP mutant of SKC1 gene.In this study,salt tolerance of 16 allelic mutants of rice salt tolerance gene SKC1 with good agronomic traits were comprehensively evaluated through salt tolerance identification at bud stage,seedling stage,booting stage and whole growth stage.The results showed that salt-tolerant mutants were 18 NY-10,18 NY-12,18 NY-14,and salt-tolerant mutants were 18 NY-2,18 NY-4,18 NY-5,18 NY-6,18 NY-7,18 NY-8.18 NY-13,18 NY-16.The results could provide germplasm resources for rice salt tolerance breeding in Ningxia.
SKC1 gene is a major salt tolerance gene cloned from rice,which is located in chromosome 1 of rice.For efficient use of SKC1salt-tolerant genes in rice breeding,and previous studies using TILLING technology to detect EMS mutant population of Ningxia main rice variety Ningjing 28,and obtained SNP mutant of SKC1 gene.In this study,salt tolerance of 16 allelic mutants of rice salt tolerance gene SKC1 with good agronomic traits were comprehensively evaluated through salt tolerance identification at bud stage,seedling stage,booting stage and whole growth stage.The results showed that salt-tolerant mutants were 18 NY-10,18 NY-12,18 NY-14,and salt-tolerant mutants were 18 NY-2,18 NY-4,18 NY-5,18 NY-6,18 NY-7,18 NY-8.18 NY-13,18 NY-16.The results could provide germplasm resources for rice salt tolerance breeding in Ningxia.
引文
[1]余美,芮孝芳.宁夏盐碱地改良利用研究进展[J].水利水电科技进展,2006,6:89-93.
[2]Lin H X,Zhu M Z,Yano M,et al.QTLs for Na+ and K+ uptake of the shoots and roots controlling rice salt tolerance[J].Theor Appl Genet,2004,108:253-260.
[3]王彩芬,马晓玲,安永平,等.TILLING技术在水稻耐盐基因SKC1突变体筛选中的应用[J].作物杂志,2013(5):66-69.
[4]殷延勃,马洪文.宁夏耐盐水稻种质资源的筛选[J].宁夏农林科技,2007,4:1-2.
[5]田蕾,王娜,张雪艳,等.盐胁迫下不同粳稻品种的形态和生理特性[J].广东农业科学,2014(23):1-6.
[6]潘晓飚,谢留杰,陈剑,等.盐胁迫下杂交水稻种子发芽特性及耐盐性评价[J].中国农学通报,2014,30(21):75-79.
[7]贾宝艳,周婵婵,孙晓雪,等.辽宁省水稻种质资源的耐盐性鉴定评价[J].作物杂志,2013(4):57-62.
[8]张所兵,张云辉,林静,等.水稻全生育期耐盐资源的初步筛选[J].中国农学通报,2013,29(36):63-68.
[2]Lin H X,Zhu M Z,Yano M,et al.QTLs for Na+ and K+ uptake of the shoots and roots controlling rice salt tolerance[J].Theor Appl Genet,2004,108:253-260.
[3]王彩芬,马晓玲,安永平,等.TILLING技术在水稻耐盐基因SKC1突变体筛选中的应用[J].作物杂志,2013(5):66-69.
[4]殷延勃,马洪文.宁夏耐盐水稻种质资源的筛选[J].宁夏农林科技,2007,4:1-2.
[5]田蕾,王娜,张雪艳,等.盐胁迫下不同粳稻品种的形态和生理特性[J].广东农业科学,2014(23):1-6.
[6]潘晓飚,谢留杰,陈剑,等.盐胁迫下杂交水稻种子发芽特性及耐盐性评价[J].中国农学通报,2014,30(21):75-79.
[7]贾宝艳,周婵婵,孙晓雪,等.辽宁省水稻种质资源的耐盐性鉴定评价[J].作物杂志,2013(4):57-62.
[8]张所兵,张云辉,林静,等.水稻全生育期耐盐资源的初步筛选[J].中国农学通报,2013,29(36):63-68.