补益营卫方对衰老表皮脂质屏障的影响
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摘要
目的研究补益营卫方对衰老表皮脂质稳态的影响,探讨其延缓皮肤衰老,改善表皮脂质稳态的可能机制。方法消化分离年轻与自然衰老小鼠表皮细胞并传代培养,其中衰老小鼠表皮细胞分为两组,一组常规培养,一组用含2.5%补益营卫方的培养基培养。RT-PCR检测各组细胞SREBP2,FASN,SCD和LXR的mRNA表达。结果与年轻组比较,老年组表皮SREBP2,FASN,SCD和LXR mRNA表达水平显著升高(P<0.05);经药物干预后,衰老表皮SREBP2,FASN,SCD和LXR mRNA表达水平显著降低(P<0.05)。结论补益营卫方可能通过对衰老表皮脂质代谢相关基因SREBP2,FASN,SCD和LXR mRNA的影响,调节衰老表皮脂质代谢,起到延缓皮肤衰老的作用。
Objective To study the effect of the Recipe of Supplementing Ying-wei on the homeostasis of the skin lipid,the mechanism of the improvement of epidermal homeostasis is discussed.Methods Digestive and separate young and natural epidermal cells of aging mice and subculture,the epidermal cells of aging mice are divided into two groups,one group of conventional cultivation,the other group was cultured in a medium containing 2.5 percent recipe of supplementing Ying-wei.The mRNA expression of SREBP2,FASN,SCDand LXR were detected by RT-PCR.Results Compared with the younger group,expression levels of the epidermal SREBP2,FASN,SCD and LXR mRNA in the older group were significantly increased(P < 0.05).The expression levels of the epidermal SREBP2,FASN,SCD and LXR mRNA were significantly reduced(P < 0.05),compared with the older group + 2.5% drug group.Conclusion The effect of SREBP2,FASN,SCD,and LXR mRNA may be used to regulate the lipid metabolism of aging epidermis,which can delay the aging of skin.
Objective To study the effect of the Recipe of Supplementing Ying-wei on the homeostasis of the skin lipid,the mechanism of the improvement of epidermal homeostasis is discussed.Methods Digestive and separate young and natural epidermal cells of aging mice and subculture,the epidermal cells of aging mice are divided into two groups,one group of conventional cultivation,the other group was cultured in a medium containing 2.5 percent recipe of supplementing Ying-wei.The mRNA expression of SREBP2,FASN,SCDand LXR were detected by RT-PCR.Results Compared with the younger group,expression levels of the epidermal SREBP2,FASN,SCD and LXR mRNA in the older group were significantly increased(P < 0.05).The expression levels of the epidermal SREBP2,FASN,SCD and LXR mRNA were significantly reduced(P < 0.05),compared with the older group + 2.5% drug group.Conclusion The effect of SREBP2,FASN,SCD,and LXR mRNA may be used to regulate the lipid metabolism of aging epidermis,which can delay the aging of skin.
引文
[1]司晓伟,周小平.补益营卫方对衰老皮肤表皮凋亡指数的影响[J].辽宁中医杂志,2016,43(5):1080.
[2]崔乐,贾焱,成志伟,等.维持皮肤屏障研究进展—脂质的分泌及组成[J].中国皮肤性病学杂志,2016,30(6):640.
[3]Kim EJ,Kim MK,Jin XJ,et al.Skin aging and photoaging alter fatty acids composition,including 11,14,17-eicosatrienoic acid,in the epidermis of human skin[J].J Korean Med Sci,2010,25(6):980.
[4]Zhang WJ,Jiang H,Zhang JL,et al.Liver X receptor activation induces apoptosis of melanoma cell through caspase pathway[J].Cancer Cell Int,2014,14(1):1b.
[5]Shen Q,Bai Y,Chang KC,et al.Liver X receptor-retinoid X receptor(LXR-RXR)heterodimer cistrome reveals coordination of LXR and AP1 signaling in keratinocytes[J].J Biol Chem,2011,286(16):14554.
[6]Chang KC,Shen Q,Oh IG,et al.Liver X receptor is a therapeutic target for photoaging and chronological skin aging[J].Mol Endocrinol,2008,22(11):2407.
[7]田晓媛.SREBP-1c在膳食脂肪酸调节肝细胞脂代谢中的作用[D].第三军医大学硕士学位论文,2012.
[8]Danesi F.Gómez-Caravaca MA,de Biase D,et al.New insight into the cholesterol-lowering effect of phytosterols in rat cardiomyocytes[J].Food Res Int,2016,89(3):1056.
[9]赵改霞.固醇调节元件结合蛋白(SREBPs)的研究进展[D].山东大学硕士学位论文,2005.
[10]DobrzyA,Ntambi J M.The role of stearoyl-Co A desaturase in the control of metabolism[J].Prostaglandins Leukot Essent Fatty Acids,2005,73(1):35.
[11]王冰冰.膨化全脂大豆对肉牛SCD基因表达及肌肉脂肪酸含量的影响[A].中国畜牧兽医学会动物营养学分会.中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集[C].中国畜牧兽医学会动物营养学分会,2016:1.
[12]Qiu YQ,Yang XF,Ma XY,et al.CIDE gene expression in adipose tissue,liver,and skeletal muscle from obese and lean pigs[J].J Zhejiang Uni Sci B,2017,18(6):492.
[13]周国利,金海国.脂肪酸合成酶基因(FASN)的研究进展[J].安徽农业科学,2008,36(9):3559.
[2]崔乐,贾焱,成志伟,等.维持皮肤屏障研究进展—脂质的分泌及组成[J].中国皮肤性病学杂志,2016,30(6):640.
[3]Kim EJ,Kim MK,Jin XJ,et al.Skin aging and photoaging alter fatty acids composition,including 11,14,17-eicosatrienoic acid,in the epidermis of human skin[J].J Korean Med Sci,2010,25(6):980.
[4]Zhang WJ,Jiang H,Zhang JL,et al.Liver X receptor activation induces apoptosis of melanoma cell through caspase pathway[J].Cancer Cell Int,2014,14(1):1b.
[5]Shen Q,Bai Y,Chang KC,et al.Liver X receptor-retinoid X receptor(LXR-RXR)heterodimer cistrome reveals coordination of LXR and AP1 signaling in keratinocytes[J].J Biol Chem,2011,286(16):14554.
[6]Chang KC,Shen Q,Oh IG,et al.Liver X receptor is a therapeutic target for photoaging and chronological skin aging[J].Mol Endocrinol,2008,22(11):2407.
[7]田晓媛.SREBP-1c在膳食脂肪酸调节肝细胞脂代谢中的作用[D].第三军医大学硕士学位论文,2012.
[8]Danesi F.Gómez-Caravaca MA,de Biase D,et al.New insight into the cholesterol-lowering effect of phytosterols in rat cardiomyocytes[J].Food Res Int,2016,89(3):1056.
[9]赵改霞.固醇调节元件结合蛋白(SREBPs)的研究进展[D].山东大学硕士学位论文,2005.
[10]DobrzyA,Ntambi J M.The role of stearoyl-Co A desaturase in the control of metabolism[J].Prostaglandins Leukot Essent Fatty Acids,2005,73(1):35.
[11]王冰冰.膨化全脂大豆对肉牛SCD基因表达及肌肉脂肪酸含量的影响[A].中国畜牧兽医学会动物营养学分会.中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集[C].中国畜牧兽医学会动物营养学分会,2016:1.
[12]Qiu YQ,Yang XF,Ma XY,et al.CIDE gene expression in adipose tissue,liver,and skeletal muscle from obese and lean pigs[J].J Zhejiang Uni Sci B,2017,18(6):492.
[13]周国利,金海国.脂肪酸合成酶基因(FASN)的研究进展[J].安徽农业科学,2008,36(9):3559.