肉牛妊娠初期血、尿中孕酮、妊娠相关糖蛋白、早孕因子的变化
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摘要
[目的]探讨肉牛妊娠初期血液和尿液中孕酮(P_4)、妊娠相关糖蛋白(PAG)、早孕因子(EPF)的变化,为肉牛早期妊娠诊断奠定基础。[方法]采集肉牛配种后第0、1、8、17、21、28、35天的血、尿液,通过ELISA检测血、尿中P_4、PAG、EPF 3种激素的浓度。[结果]在配后0～35 d,妊娠与非妊娠母牛血、尿中P_4和PAG浓度变化平缓,而EPF差异较明显,3种激素血液中浓度均高于尿液中。其中,妊娠牛血液中EPF浓度在配后第8天显著(P<0.05)高于非妊娠牛。[结论]血、尿中EPF浓度变化能反映肉牛的妊娠状态。
[Objective]The aim of this study was to investigate the changes of progesterone(P_4), pregnancy-associated glycoprotein(PAG) and early pregnancy factor(EPF) in blood and urine of beef cattle during the early pregnancy, and to lay a foundation for early pregnancy diagnosis of beef cattle. [Method]The blood and urine of beef cattle were collected at 0, 1, 8, 17, 21, 28 and 35 days after mating. The concentrations of P_4, PAG and EPF in blood and urine were detected by ELISA method. [Results]The results showed that there was no significant difference in the concentration of P_4 and PAG in blood and urine between pregnant and non-pregnant cattles from 0 to 35 days after mating, but the difference in EPF was obvious, and the concentration of three hormones in blood was higher than that in urine. The concentration of EPF in blood of pregnant cattle was significantly higher than that of non-pregnant cattle on the 8 th day(P< 0.05). [Conclusion]The concentration of EPF in blood and urine could reflect the pregnancy status of beef cattle better.
[Objective]The aim of this study was to investigate the changes of progesterone(P_4), pregnancy-associated glycoprotein(PAG) and early pregnancy factor(EPF) in blood and urine of beef cattle during the early pregnancy, and to lay a foundation for early pregnancy diagnosis of beef cattle. [Method]The blood and urine of beef cattle were collected at 0, 1, 8, 17, 21, 28 and 35 days after mating. The concentrations of P_4, PAG and EPF in blood and urine were detected by ELISA method. [Results]The results showed that there was no significant difference in the concentration of P_4 and PAG in blood and urine between pregnant and non-pregnant cattles from 0 to 35 days after mating, but the difference in EPF was obvious, and the concentration of three hormones in blood was higher than that in urine. The concentration of EPF in blood of pregnant cattle was significantly higher than that of non-pregnant cattle on the 8 th day(P< 0.05). [Conclusion]The concentration of EPF in blood and urine could reflect the pregnancy status of beef cattle better.
引文
[1] 张昱,王海东,贺俊平,等.妊娠相关糖蛋白及其在反刍动物早期妊娠诊断中的应用[J].中国畜牧兽医,2015,42(3):764-768.
[2] 贺加双,马卫明,邓立新,等.玫瑰花环抑制实验检测肉牛早孕因子(EPF)活性[J].中国牛业科学,2011,37(3):11-13.
[3] 郭爱珍,杨利国.牛奶孕酮酶联免疫测定技术及其在奶牛繁殖上的应用[J].中国奶牛,1989(5):39-41.
[4] 张寿,王应安,常兰,等.不同繁殖类型母牦牛发情周期中血浆P4和17β-E2水平的测定[J].中国兽医科技,2003,33(4):59-61.
[5] 谢炳坤.广西黄牛和水牛发情周期血清生殖激素变化规律的研究[D].南宁:广西大学,2005.
[6] 刘远,吴贤锋,陈鑫珠,等.福清山羊和戴云山羊发情周期内生殖激素分泌规律研究[J].福建农业学报,2017(10):1062-1065.
[7] 赵鹏鹏.雌性大熊猫繁殖期尿中雌二醇、孕酮水平变化的研究[D].陕西杨凌:西北农林科技大学,2012.
[8] 陈学进,陈永昌,陈丽华,等.雌性猕猴尿中生殖激素的变化[J].东北林业大学学报,2004,32(3):59-60.
[9] 张佰忠,雷虹,李晟,等.湘西黄牛发情周期生殖激素变化规律[J].黑龙江畜牧兽医,2016(18):75-77.
[10] 王宏娟.肉用母牛发情周期体内生殖激素变化规律与早期妊娠诊断技术的研究[D].长春:吉林大学, 2012.
[11] Garbayo J M,Remy B,Alabart J L,et al Isolation and partial characterization of a pregnancy-associated glycoprotein family from the goat placenta[J].Biology of Reproduction,1998,58(1):109-15.
[12] Zoli A P,Guilbault L A,Delahaut P,et al.Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum:Its application for pregnancy diagnosis[J].Biology of Reproduction,1992,46(1):83-92.
[13] Giordano J O,Guenther J N,Jr Lopes G,et al.Changes in serum pregnancy-associated glycoprotein,pregnancy-specific protein B,and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows[J].Journal of Dairy Science,2012,95(2):683-697.
[14] 贾斌,李仁燕,郭玉强,等.家畜早孕因子的研究现状及其应用[J].石河子大学学报:自然科学版,2007,25(6):57-61.
[15] Walsh A,Whelan D,Bielanowicz A,et al.Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10),in the reproductive tract and in capacitating spermatozoa in the male mouse[J].Biology of Reproduction,2008,78(6):983-993.
[16] Shamaei-Tousi A,D'Aiuto F,Nibali L,et al.Differential regulation of circulating levels of molecular chaperones in patients undergoing treatment for periodontal disease[J].Plos One,2007,2(11):e1198.
[17] 左海洋,陈晓丽,蔡勇,等.奶牛早期妊娠诊断技术研究进展[J].畜牧兽医学报,2014,45(10):1584-1591.
[18] Romano J E,Thompson J A,Kraemer D C,et al.Early pregnancy diagnosis by palpation per rectum: Influence on embryo/fetal viability in dairy cattle[J].Theriogenology,2007,67(3):486-493.
[19] 李梦林.尿液是疾病标志物的理想来源[D].北京:北京协和医学院,2014.
[20] 卫泽珍,崔媛媛.内分泌学分析法在圈养野生动物研究中的应用[J].野生动物学报,2017,38(3):524-528.
[21] 蔡正华,赵尚吉,方樟明,等.妊娠母猪尿雌酮水平以及作早期妊娠诊断[J].当代畜牧,1989(2):34-36.
[22] Evans K L,Kasman L H,Hughes J P,et al.Pregnancy diagnosis in the domestic horse through direct urinary estrone conjugate analysis[J].Theriogenology,1984,22(6):615-620.
[23] Volkery J,Gottschalk J,Sobiraj A,et al.Progesterone, pregnanediol-3-glucuronide,relaxin and oestrone sulphate concentrations in saliva,milk and urine of female alpacas (Vicugna pacos) and their application in pregnancy diagnosis[J].Veterinary Record,2010,171(8):195.
[24] Karen A,Sousa N M D,Beckers J F,et al.Comparison of a commercial bovine pregnancy-associated glycoprotein ELISA test and a pregnancy-associated glycoprotein radiomimmunoassay test for early pregnancy diagnosis in dairy cattle[J].Animal Reproduction Science,2015,159:31-37.
[2] 贺加双,马卫明,邓立新,等.玫瑰花环抑制实验检测肉牛早孕因子(EPF)活性[J].中国牛业科学,2011,37(3):11-13.
[3] 郭爱珍,杨利国.牛奶孕酮酶联免疫测定技术及其在奶牛繁殖上的应用[J].中国奶牛,1989(5):39-41.
[4] 张寿,王应安,常兰,等.不同繁殖类型母牦牛发情周期中血浆P4和17β-E2水平的测定[J].中国兽医科技,2003,33(4):59-61.
[5] 谢炳坤.广西黄牛和水牛发情周期血清生殖激素变化规律的研究[D].南宁:广西大学,2005.
[6] 刘远,吴贤锋,陈鑫珠,等.福清山羊和戴云山羊发情周期内生殖激素分泌规律研究[J].福建农业学报,2017(10):1062-1065.
[7] 赵鹏鹏.雌性大熊猫繁殖期尿中雌二醇、孕酮水平变化的研究[D].陕西杨凌:西北农林科技大学,2012.
[8] 陈学进,陈永昌,陈丽华,等.雌性猕猴尿中生殖激素的变化[J].东北林业大学学报,2004,32(3):59-60.
[9] 张佰忠,雷虹,李晟,等.湘西黄牛发情周期生殖激素变化规律[J].黑龙江畜牧兽医,2016(18):75-77.
[10] 王宏娟.肉用母牛发情周期体内生殖激素变化规律与早期妊娠诊断技术的研究[D].长春:吉林大学, 2012.
[11] Garbayo J M,Remy B,Alabart J L,et al Isolation and partial characterization of a pregnancy-associated glycoprotein family from the goat placenta[J].Biology of Reproduction,1998,58(1):109-15.
[12] Zoli A P,Guilbault L A,Delahaut P,et al.Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum:Its application for pregnancy diagnosis[J].Biology of Reproduction,1992,46(1):83-92.
[13] Giordano J O,Guenther J N,Jr Lopes G,et al.Changes in serum pregnancy-associated glycoprotein,pregnancy-specific protein B,and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows[J].Journal of Dairy Science,2012,95(2):683-697.
[14] 贾斌,李仁燕,郭玉强,等.家畜早孕因子的研究现状及其应用[J].石河子大学学报:自然科学版,2007,25(6):57-61.
[15] Walsh A,Whelan D,Bielanowicz A,et al.Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10),in the reproductive tract and in capacitating spermatozoa in the male mouse[J].Biology of Reproduction,2008,78(6):983-993.
[16] Shamaei-Tousi A,D'Aiuto F,Nibali L,et al.Differential regulation of circulating levels of molecular chaperones in patients undergoing treatment for periodontal disease[J].Plos One,2007,2(11):e1198.
[17] 左海洋,陈晓丽,蔡勇,等.奶牛早期妊娠诊断技术研究进展[J].畜牧兽医学报,2014,45(10):1584-1591.
[18] Romano J E,Thompson J A,Kraemer D C,et al.Early pregnancy diagnosis by palpation per rectum: Influence on embryo/fetal viability in dairy cattle[J].Theriogenology,2007,67(3):486-493.
[19] 李梦林.尿液是疾病标志物的理想来源[D].北京:北京协和医学院,2014.
[20] 卫泽珍,崔媛媛.内分泌学分析法在圈养野生动物研究中的应用[J].野生动物学报,2017,38(3):524-528.
[21] 蔡正华,赵尚吉,方樟明,等.妊娠母猪尿雌酮水平以及作早期妊娠诊断[J].当代畜牧,1989(2):34-36.
[22] Evans K L,Kasman L H,Hughes J P,et al.Pregnancy diagnosis in the domestic horse through direct urinary estrone conjugate analysis[J].Theriogenology,1984,22(6):615-620.
[23] Volkery J,Gottschalk J,Sobiraj A,et al.Progesterone, pregnanediol-3-glucuronide,relaxin and oestrone sulphate concentrations in saliva,milk and urine of female alpacas (Vicugna pacos) and their application in pregnancy diagnosis[J].Veterinary Record,2010,171(8):195.
[24] Karen A,Sousa N M D,Beckers J F,et al.Comparison of a commercial bovine pregnancy-associated glycoprotein ELISA test and a pregnancy-associated glycoprotein radiomimmunoassay test for early pregnancy diagnosis in dairy cattle[J].Animal Reproduction Science,2015,159:31-37.