生防细菌NJ13与化学农药复配对人参镰刀菌根腐病菌的抑制作用
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摘要
为了明确甲基营养型芽孢杆菌NJ13及与化学药剂混配对人参根腐病菌的毒力及其抑制作用,该研究采用生长速率法、孙云沛法测定分析了菌株NJ13及与化学药剂混配对人参根腐病菌的室内抑菌效果及共毒系数;并采用混合培养法、对峙培养法及显微观察分别测定了菌株NJ13对病原菌产孢量、孢子萌发、菌丝生长量、菌丝形态的抑制作用。室内毒力测定结果表明,与4种化学杀菌剂相比,菌株NJ13对人参根腐病菌抑制效果最好,EC_(50)为0. 071 mg·L~(-1);菌株NJ13分别与丙环唑、苯醚甲环唑在配比为1∶4～4∶1对病原菌抑菌效果均具有增效作用,抑菌最佳配比皆为1∶1,共毒系数分别为848. 70,859. 73。菌株NJ13对人参根腐病菌的产孢量、孢子萌发、菌丝生长均有抑制作用。
This study was aimed to clarify the toxicity indoor and inhibition effect of biocontrol strain NJ13 and its mixture with chemical fungicides against Fusarium solani causing ginseng root rot. The method of mycelial growth rate and Sun Yunpei method were used to determine the indoor toxicity and co-toxicity coefficient of strain NJ13 and their mixture with chemical pesticides against F. solani. The dual culture assay method,mixed culture method and microscopic observation were used to determine the sporulation and germination of spores and mycelial growth and morphological change of hyphae of F. solani treated by strain NJ13. The results of toxicity indoor showed that strain NJ13 had the best inhibitory effect on pathogen,and its EC_(50) value was 0. 071 mg·L~(-1). It was all synergistic for antifungal effect that strain NJ13 was mixed with propiconazole and difenoconazole respectively with a range from 1 ∶4 to 4 ∶1( volume ratio). Both of optimal ratios were 1 ∶1,and the co-toxicity coefficients were 848. 70 and 859. 73,respectively. The strain NJ13 could inhibit the sporulation,germination and mycelial growth of F. solani. The biocontrol strain NJ13 had an inhibition effect on F. solani,and the optimal antifungal ratio of strain NJ13 mixed with propiconazole and difenoconazole was obtained.
This study was aimed to clarify the toxicity indoor and inhibition effect of biocontrol strain NJ13 and its mixture with chemical fungicides against Fusarium solani causing ginseng root rot. The method of mycelial growth rate and Sun Yunpei method were used to determine the indoor toxicity and co-toxicity coefficient of strain NJ13 and their mixture with chemical pesticides against F. solani. The dual culture assay method,mixed culture method and microscopic observation were used to determine the sporulation and germination of spores and mycelial growth and morphological change of hyphae of F. solani treated by strain NJ13. The results of toxicity indoor showed that strain NJ13 had the best inhibitory effect on pathogen,and its EC_(50) value was 0. 071 mg·L~(-1). It was all synergistic for antifungal effect that strain NJ13 was mixed with propiconazole and difenoconazole respectively with a range from 1 ∶4 to 4 ∶1( volume ratio). Both of optimal ratios were 1 ∶1,and the co-toxicity coefficients were 848. 70 and 859. 73,respectively. The strain NJ13 could inhibit the sporulation,germination and mycelial growth of F. solani. The biocontrol strain NJ13 had an inhibition effect on F. solani,and the optimal antifungal ratio of strain NJ13 mixed with propiconazole and difenoconazole was obtained.
引文
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[12]农倩,黎起秦,袁高庆,等.内生细菌菌株B196与戊唑醇混配对水稻纹枯病的防治作用[J].农药,2010,49(11):838.
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[3]邵天蔚,李勇.利用生防微生物防治人参根部病害的研究进展[J].中国现代中药,2016,18(3):383.
[4]常有宏,刘邮洲,王宏,等.嘧霉胺与枯草芽孢杆菌B-916协同防治梨黑斑病[J].江苏农业学报,2010,26(6):1227.
[5]陈志谊,任海英,刘永锋,等.戊唑醇和枯草芽孢杆菌协同作用防治蚕豆枯萎病及增效机理初探[J].农药学学报,2002,4(4):40.
[6]刘淑娟,陈秀蓉,袁宏波,等.芽孢杆菌(Bacillus spp.)与甲基硫菌灵混配对茄腐镰孢菌(Fusarium solani)的抑制作用[J].植物保护,2008,34(5):149.
[7]姜云,尹望,陈长卿,等.人参内生拮抗细菌NJ13的鉴定及发酵条件[J].农药,2012,52(2):97.
[8]胡飞,胡本进,李昌春,等.解淀粉芽孢杆菌JDF-6·井冈霉素A组合物对油茶炭疽病室内毒力测定及林间试验[J].农药,2015,54(7):537.
[9]吉沐祥,姚克兵,李国平,等.枯草芽孢杆菌DJ-6与吡唑醚菌酯及其混配对草莓枯萎病的室内抑菌活性与田间促生防病效果[J].农业科学与技术,2014(11):2020.
[10]刘磊,梁昌聪,杨腊英,等.生防芽孢杆菌与化学杀菌剂混配对香蕉枯萎病菌室内毒力测定[J].中国南方果树,2013,42(6):66.
[11]刘淑娟,陈秀蓉,杨成德,等.生防芽孢杆菌与扑海因混配对番茄早疫病菌的抑制作用[J].甘肃农业大学学报,2007,42(1):49.
[12]农倩,黎起秦,袁高庆,等.内生细菌菌株B196与戊唑醇混配对水稻纹枯病的防治作用[J].农药,2010,49(11):838.
[13]赵广,郭堂勋,李其利,等.枯草芽孢杆菌9A与4种药剂混配对杧果炭疽病防控研究[J].南方园艺,2015,26(5):1.