干旱胁迫下水稻转录因子表达变化
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摘要
本研究是探索在干旱应激下水稻转录因子的变化及可能的调控机制。本研究整合GEO数据库中关于水稻耐旱研究的基因芯片数据,通过TAC软件对基因芯片数据分析筛选出差异基因,利用在线数据库DAVID 6.8和Plant TF DB筛选出相应的转录因子。敏感品种有9个转录因子表达方向一致(同时上调表达的有7个转录因子,下调的有2个转录因子),耐旱品种有34个转录因子表达方向一致(同时上调表达的有23个转录因子,下调表达的有11个转录因子)。敏感品种和耐旱品种间有8个表达方向一致的重叠转录因子。干旱胁迫会显著影响转录因子的表达,不同水稻品种中,这些转录因子的表达既有较强的品种特异性,也有部分相似性。水稻可通过诱导和抑制转录因子的表达,来提高自身抗旱能力。
To investigate the change of transcription factors' response to drought stress in rice and their potential regulatory mechanism, the data of gene chips on drought resistance of rice from GEO database were integrated. The differential genes were screened out by analyzing the data of gene chips with TAC software.And the relative transcription factors were selected with online database DAVID 6.8 and Plant TF DB. 9 transcription factor genes(7 up-regulated and 2 down-regulated at the transcription level) in drought-susceptible varieties and 34(23 up-regulated and 11 down-regulated at the transcription level) in drought-tolerant varieties had the same direction of expression. Between drought-susceptible varieties and drought-tolerant varieties, 8 overlapping transcription factors had the same direction of expression. Drought stress affected the expression of transcription factors obviously. In different varieties, these transcription factors had strong variety specificity, and some similarity. Rice can improve the drought resistance by inducing and inhibiting the expression of transcription factors.
To investigate the change of transcription factors' response to drought stress in rice and their potential regulatory mechanism, the data of gene chips on drought resistance of rice from GEO database were integrated. The differential genes were screened out by analyzing the data of gene chips with TAC software.And the relative transcription factors were selected with online database DAVID 6.8 and Plant TF DB. 9 transcription factor genes(7 up-regulated and 2 down-regulated at the transcription level) in drought-susceptible varieties and 34(23 up-regulated and 11 down-regulated at the transcription level) in drought-tolerant varieties had the same direction of expression. Between drought-susceptible varieties and drought-tolerant varieties, 8 overlapping transcription factors had the same direction of expression. Drought stress affected the expression of transcription factors obviously. In different varieties, these transcription factors had strong variety specificity, and some similarity. Rice can improve the drought resistance by inducing and inhibiting the expression of transcription factors.
引文
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[16]孙滨,占小登,曹立勇,等.水稻AP2/ERF转录因子的研究进展[J].农业生物技术学报,2017(11):1860-1869.
[17]Lu Jing,Ju H P,Zhou G X,et al.An EAR-motif-containing ERFtranscription factor affects herbivore-induced signaling,defense and resistance in rice[J].The Plant Journal,2011,68(4):583-596.
[18]刘梦佳,李海峰.水稻WRKY转录因子家族研究进展[J].河南农业科学,2016,45(3):1-8.
[19]Shen H S,Liu C T,Zhang Y,et al.OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice[J].Plant Molecular Biology,2012,80(3):241-253.
[20]Raineri J,Wang S H,Peleg Z,et al.The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress[J].Plant Molecular Biology,2015,88(4-5):401-413.
[2]Miura K,Ikeda M,Matsubara A,et al.OsSPL14 promotes panicle branching and higher grain productivity in rice[J].Nature Genetics,2010,42(6):545-549.
[3]You J,Zong W,Hu H H,et al.A Stress-Responsive NAC1-Regulated Protein Phosphatase Gene Rice Protein Phosphatase18Modulates Drought and Oxidative Stress Tolerance through Abscisic Acid-Independent Reactive Oxygen Species Scavenging in Rice[J].Plant Physiology,2014,166(4):2100-2114.
[4]Feng L,Gao Z R,Xiao G Q,et al.Leucine-Rich Repeat ReceptorLike Kinase FON1 Regulates Drought Stress and Seed Germination by Activating the Expression of ABA-Responsive Genes in Rice[J].Plant Molecular Biology Reporter,2014,32(6):1158-1168.
[5]刘永巍,田红刚,李春光,等.水稻抗旱研究进展[J].北方水稻,2015,45(2):76-80.
[6]Hadiart T,Tran L S.Progress studies of drought responsive genes in rice[J].Plant Cell Reports,2011,30(3):297-310.
[7]Lee D K,Jung H,Jang G P,et al.Overexpression of the OsERF71transcription factor alters rice root structure and drought resistance[J].Plant Physiology,2016,172(1):575-588.
[8]Jisha V,Lavanya D,Jyothilakshmi V,et al.Overexpression of an AP2/ERF type transcription factor OsEREBP1 confers biotic and abiotic stress tolerance in Rice[J].PLoS ONE,2015.10(6):e0127831.
[9]Li C,Ng K Y,Fan L M,et al.MYB transcription factors,active players in abiotic stress signaling[J].Environmental&Experimental Botany,2015,114:80-91.
[10]Zong W,Tang N,Yang J,et al.Feedback Regulation of ABASignaling and Biosynthesis by a b ZIP Transcription Factor Targets Drought-Resistance-Related Genes[J].Plant Physiology,2016,171(4):2810-2825.
[11]Chen H,Chen W,Zhou J L,et al.Basic leucine zipper transcription factor OsbZIP16 positively regulates drought resistance in rice[J].Plant Science,2012(193):8-17.
[12]Xiong H Y,Li J J,Liu P L,et al.Overexpression of OsMYB48-1,a novel MYB-related transcription factor,enhances drought and salinity tolerance in rice[J].PLoS ONE,2014,9(3):e92913.
[13]Huang L,Hong Y B,Zhang H J,et al.Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance[J].BMC Plant Biology,2016,16:203-241.
[14]刘文文,李文学.植物bHLH转录因子研究进展[J].生物技术进展,2013(1):7-11.
[15]Todaka D,Nakashima K,Maruyama K,et al.Rice phytochromeinteracting factor-like protein OsPIL1 functions as a key regulator of internode elongation and induces a morphological response to drought stress[J].Proceedings of the National Academy of Sciences,2012,109(39):15947-15952.
[16]孙滨,占小登,曹立勇,等.水稻AP2/ERF转录因子的研究进展[J].农业生物技术学报,2017(11):1860-1869.
[17]Lu Jing,Ju H P,Zhou G X,et al.An EAR-motif-containing ERFtranscription factor affects herbivore-induced signaling,defense and resistance in rice[J].The Plant Journal,2011,68(4):583-596.
[18]刘梦佳,李海峰.水稻WRKY转录因子家族研究进展[J].河南农业科学,2016,45(3):1-8.
[19]Shen H S,Liu C T,Zhang Y,et al.OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice[J].Plant Molecular Biology,2012,80(3):241-253.
[20]Raineri J,Wang S H,Peleg Z,et al.The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress[J].Plant Molecular Biology,2015,88(4-5):401-413.