外源海藻糖对花绒寄甲成虫存活和耐寒性的影响
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摘要
【目的】探讨取食不同浓度外源海藻糖对室内饲养的花绒寄甲Dastarcus helophoroides成虫存活和耐寒性的影响。【方法】在室内分别用含3%, 6%和9%海藻糖的半人工饲料饲养花绒寄甲成虫,以取食不含海藻糖的半人工饲料的花绒寄甲成虫为对照组,统计饲养10周后的存活率,测定未经低温处理和10℃低温处理3 d的成虫过冷却点和含水量。【结果】取食含6%海藻糖的半人工饲料的花绒寄甲成虫存活率最高,为86.67%。不管是否经低温处理,取食含9%海藻糖的半人工饲料的成虫与取食含3%和6%海藻糖的半人工饲料的成虫以及不含海藻糖的半人工饲料的成虫(对照)相比,其过冷却点均最低,其中未经低温处理的成虫过冷却点为-19.30℃,而经低温处理的成虫过冷却点为-21.60℃。低温处理对取食不含海藻糖的半人工饲料的成虫的含水量有显著影响,而对取食含海藻糖的半人工饲料的成虫含水量无显著影响。【结论】外源海藻糖对花绒寄甲成虫的存活和过冷却点有显著影响,可以利用外源海藻糖提高室内饲养花绒寄甲成虫的存活率和耐寒性。
【Aim】 This study aims to evaluate the influence of feeding different concentrations of exogenous trehalose on the survival and cold hardiness of adult Dastarcus helophoroides, an important and most effective natural enemy of longhorned beetles. 【Methods】 D. helophoroides adults were fed on a semi-artificial diets containing 3%, 6% and 9% trehalose, respectively, in the laboratory, and those fed on the semi-artificial diet without trehalose were used as the control group. After 10 weeks, the survival rate of adults was calculated. In addition, the supercooling point and water content of adults not subjected to low temperature treatment and subjected to low temperature treatment(10℃ for 3 d) were measured. 【Results】 The survival rate of D. helophoroides adults fed on the semi-artificial diets containing 6% trehalose was the highest(86.67%) among all the treatments. Whether subjected to low temperature treatment or not, D. helophoroides adults fed on the semi-artificial diet containing 9% trehalose had the lowest supercooling point as compared to those fed on the semi-artificial diet containing 3% and 6% trehalose and without trehalose(the control): the supercooling point of adults unsubjected to low temperature was-19.30℃, while that of adults subjected to low temperature treatment was-21.60℃. Low temperature treatment had a significant effect on the water content of adults fed on the diet without trehalose but no significant effect on the water content of adults fed on the semi-artificial diet containing trehalose. 【Conclusion】 Exogenous trehalose has significant influence on the survival and cold hardiness of D. helophoroides adults. Thus exogenous trehalose can be utilized to improve the survival rate and cold hardiness of D. helophoroides adults in laboratory rearing.
【Aim】 This study aims to evaluate the influence of feeding different concentrations of exogenous trehalose on the survival and cold hardiness of adult Dastarcus helophoroides, an important and most effective natural enemy of longhorned beetles. 【Methods】 D. helophoroides adults were fed on a semi-artificial diets containing 3%, 6% and 9% trehalose, respectively, in the laboratory, and those fed on the semi-artificial diet without trehalose were used as the control group. After 10 weeks, the survival rate of adults was calculated. In addition, the supercooling point and water content of adults not subjected to low temperature treatment and subjected to low temperature treatment(10℃ for 3 d) were measured. 【Results】 The survival rate of D. helophoroides adults fed on the semi-artificial diets containing 6% trehalose was the highest(86.67%) among all the treatments. Whether subjected to low temperature treatment or not, D. helophoroides adults fed on the semi-artificial diet containing 9% trehalose had the lowest supercooling point as compared to those fed on the semi-artificial diet containing 3% and 6% trehalose and without trehalose(the control): the supercooling point of adults unsubjected to low temperature was-19.30℃, while that of adults subjected to low temperature treatment was-21.60℃. Low temperature treatment had a significant effect on the water content of adults fed on the diet without trehalose but no significant effect on the water content of adults fed on the semi-artificial diet containing trehalose. 【Conclusion】 Exogenous trehalose has significant influence on the survival and cold hardiness of D. helophoroides adults. Thus exogenous trehalose can be utilized to improve the survival rate and cold hardiness of D. helophoroides adults in laboratory rearing.
引文
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