HPLC法测定不同产地女贞子中红景天苷、女贞苷、特女贞苷的含量
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摘要
目的:针对不同产地的女贞子建立红景天苷、女贞苷、特女贞苷高效液相色谱测定方法。方法:采用高效液相色谱法,色谱条件:Aglient Eclipse XDB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以水-乙腈为流动相,梯度洗脱,体积流量1.0 mL/min,检测波长282 nm,柱温25℃。结果:10个产地女贞子药材红景天苷、女贞苷、特女贞苷含量分别在2.06～4.34 mg/g、2.52～28.73 mg/g、8.86～46.84 mg/g范围。结论:所建立的高效液相测定方法可用于女贞子药材的质量控制。
Objective: To study the determination of salidroside, ligustroflavone, and specnuezhenide of Ligustrum lucidum in different habitats by HPLC. Methods: HPLC was used to determine the contents. The analytical column was Aglient Eclipse XDB-C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was composed of acetonitrile and water with gradient elution. The detection wave length was set at 282 nm, the flow rate was 1.0 mL/min and the column temperature was maintained at 25 ℃. Results: The contents of salidroside, ligustroflavone, specnuezhenide of Ligustrum lucidum in 10 places were 2.06～4.34 mg/g, 2.52～28.73 mg/g, and 8.86～46.84 mg/g respectively. Conclusion: The HPLC method can be used for the quality control of Ligustrum lucidum.
Objective: To study the determination of salidroside, ligustroflavone, and specnuezhenide of Ligustrum lucidum in different habitats by HPLC. Methods: HPLC was used to determine the contents. The analytical column was Aglient Eclipse XDB-C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was composed of acetonitrile and water with gradient elution. The detection wave length was set at 282 nm, the flow rate was 1.0 mL/min and the column temperature was maintained at 25 ℃. Results: The contents of salidroside, ligustroflavone, specnuezhenide of Ligustrum lucidum in 10 places were 2.06～4.34 mg/g, 2.52～28.73 mg/g, and 8.86～46.84 mg/g respectively. Conclusion: The HPLC method can be used for the quality control of Ligustrum lucidum.
引文
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[16] 许浚,游飞祥,袁雪海,等.女贞子HPLC指纹图谱及多指标成分测定[J].中草药,2017,48(20):4198-4202.
[17] 韩疏影,项洋洋,王星,等.女贞子“一测多评”法测定[J].中国实验方剂学杂志,2016,22(23):73-77.
[18] 崔蕴慧,樊化,利弈成,等.女贞子指纹图谱研究及红景天苷、特女贞苷的含量测定[J].时珍国医国药,2014,25(12):2854-2857.
[19] 杨光明,陈晓红,刘路,等.女贞子生品及炮制品的HPLC指纹图谱研究[J].中草药,2016,47(5):760-766.
[20] 任爽,李铮,王京辉,等.中药女贞子饮片中8个成分的含量比较研究[J].药物分析杂志,2015,35(8):1393-1402.
[21] 邹小燕,李庆磊,张迪文,等.黄酒产地对酒炙女贞子中红景天苷、特女贞苷和女贞苷的影响[J].中医学报,2017,32(10):1962-1965.
[2] 黄晓君,殷志琦.女贞子的化学成分研究[J].中国中药杂志,2010,35(7):861-864.
[3] Liangliang Gao,Chun Li.Ligustri Lucidi Fructus as a traditional Chinese medicine:a review of its photochemistry and pharmacology[J].Natural Product Research,2014(10):1-20.
[4] Liangliang Gao,Xiaoqian Liu.A rare secoiridoid Dimer Derivative from Ligustri Lucidi fructus[J].Records of Natural Products,2015,9(3):323-328.
[5] 霍雨佳,岳琳,刘颖,等.酒制对女贞子饮片主要化学成分含量的影响[J].中国实验方剂学杂志,2018,24(1):26-30.
[6] Hui Li,Weifeng Yao,Qinan Liu,et al.Application of UHPLC-ESI-QTOF-MS to Identify Multiple Constituents in Processed Products of the Herbal Medicine Ligustri Lucidi Fructus[J].Molecules,2017(22):689.
[7] 战美,周家杰,吴琪聪,等.女贞子成分抗骨质疏松的潜在作用靶点虚拟筛选[J].世界中医药,2017,12(7):1693-1697.
[8] Lili Wang,Rufeng Ma,Yubo Guo,et al.Antioxidant Effect of Fructus Ligustri Lucidi Aqueous Extract in Ovariectomized Rats Is Mediated through Nox4-ROS-NF-κB Pathway[J].Frontiers in Phaemacology,2017(8):266.
[9] Yan Zhang.Combination therapy of Chinese herbal medicine Fructus Ligustri Lucidi with high calcium diet on calcium imbalance induced by ovariectomy in mice[J].Pharmaceutical Biology,2015,53(7):1082-1085.
[10] 姜华,高原,杨景明,等.源于“整体观”思想的中药质量评价方法研究概述[J].中国中药杂志,2015,40(6):1027-1031.
[11] 李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3104.
[12] 季申,王柯,胡青,等.基于有效性和安全性相关的中药质量控制方法的建立[J].世界科学技术-中医药现代化,2014,16(3):502-505.
[13] 邹纯才,鄢海燕.我国中药色谱指纹图谱相似度评价方法30年(1988-2017年)研究进展与展望[J].中国中药杂志,2018,43(10):1969-1977.
[14] Wu YL,Piao DM,Han XH,et al.Protective effects of salidroside against acetaminophen-induced toxicity in mice[J].Biological and Pharmaceutical Bulletin,2008,31(8):1523-1529.
[15] 潘志浩,黄清松.红景天苷对大鼠CCl4肝纤维化模型干预作用研究[J].新余学院学报,2012,17(2):104-106.
[16] 许浚,游飞祥,袁雪海,等.女贞子HPLC指纹图谱及多指标成分测定[J].中草药,2017,48(20):4198-4202.
[17] 韩疏影,项洋洋,王星,等.女贞子“一测多评”法测定[J].中国实验方剂学杂志,2016,22(23):73-77.
[18] 崔蕴慧,樊化,利弈成,等.女贞子指纹图谱研究及红景天苷、特女贞苷的含量测定[J].时珍国医国药,2014,25(12):2854-2857.
[19] 杨光明,陈晓红,刘路,等.女贞子生品及炮制品的HPLC指纹图谱研究[J].中草药,2016,47(5):760-766.
[20] 任爽,李铮,王京辉,等.中药女贞子饮片中8个成分的含量比较研究[J].药物分析杂志,2015,35(8):1393-1402.
[21] 邹小燕,李庆磊,张迪文,等.黄酒产地对酒炙女贞子中红景天苷、特女贞苷和女贞苷的影响[J].中医学报,2017,32(10):1962-1965.