玉屏风散对过敏性皮肤病模型大鼠的影响
|
摘要
目的:观察玉屏风散对过敏性皮肤病动物模型的影响,探讨其治疗过敏性皮肤病的作用机制。方法:被动致敏大鼠模型31只,随机分为模型组7只、醋酸泼尼松组7只、玉屏风散低剂量组7只、玉屏风散高剂量组7只,另有3只作为血清来源,分别灌服生理盐水(10 m L·kg~(~(-1)))、醋酸泼尼松片水溶液(2.5 mg·kg~(~(-1)))及玉屏风颗粒水溶液(低剂量组1.35g·kg~(~(-1))、高剂量组4.05 g·kg~(~(-1)))。3 d后,采用抗原攻击并鼠尾静脉注入伊文思蓝,测定体表渗出的蓝斑直径,并测定脾脏指数。结果:连续灌胃给药3 d后,与模型组比较,玉屏风散低剂量组和高剂量组对被动皮肤过敏大鼠背部的蓝斑直径均有不同程度的减小;低剂量组1∶5稀释抑制率为11.87%,高剂量组1∶5稀释抑制率为13.76%,两组抑制率比较,差异有统计学意义(P<0.05)。对1∶10稀释的血清处皮斑直径均有缩小作用,低剂量组为9.80%,高剂量组为11.54%,两组比较,差异无统计学意义(P>0.05)。模型组大鼠脾脏指数为55.72±14.00,醋酸泼尼松组为37.44±9.65,两组比较,差异有统计学意义(P<0.05);玉屏风散高剂量组脾脏指数为44.04±11.04,与模型组比较,差异无统计学意义(P>0.05)。结论:玉屏风散对Ⅰ型变态反应引起的过敏性皮肤病具有良好的抗过敏作用,并且没有明显的免疫抑制不良反应。
Objective:To observe the effect of Yupingfeng Powder on allergic dermatosis animal model and to explore its treatment mechanism of allergic dermatosis. Methods:Making passive induced rat model of sensitive 31 were randomly divided into model group 7 rats,control group( n = 7),Yupingfeng Powder middle dose group( n = 7),Yupingfeng Powder high dose group( n = 7),and another 3 only as a source of serum were given service physiological saline(10 mL ·kg~(-1)),acetic acid throwing prednisone tablets in aqueous solution(2. 5 mg·kg~(-1)) and Yupingfeng Powder particles in aqueous solution(1. 35 g·kg~(-1) in the dose group,high dose group 4. 05 g·kg~(-1)) for 3 days. In this study,the rats were assaulted by the antigen. Then the Evans blue was injected in to rats tail vein. The effusion size( diameter) of the Evans blue from the skin and splenic index were measured to evaluate the immune reaction. Results:After three days of intragastric administration. Compared with the model group,middle dose group and high dose group Yupingfeng Powder made the size of the blue stain in the back of rats was decreased. The inhibition rate of middle dose group 1∶5 diluted to 11. 87%,high dose group 1∶5 diluted to 13. 76%( P < 0. 05) and 1∶10 diluted serum were reduced,but no significant difference( The inhibition rate of middle dose group 9. 80%,high dose group 11. 54%,P > 0. 05). The index of spleen index in the model group was 55. 72 ± 14. 00,and the prednisone acetate group was 37. 44 ± 9. 65. The difference between the two groups was statistically significant( P < 0. 05),and the large dose group in Yupingfeng Powder was 44. 04 ± 11. 04,and compared with the model group,the difference was not statistically significant( P > 0. 05). Conclusion:Yupingfeng Powder in the treatment of allergic skin diseases caused by type I allergic reaction has good anti allergic effect,and no obvious side effect.
Objective:To observe the effect of Yupingfeng Powder on allergic dermatosis animal model and to explore its treatment mechanism of allergic dermatosis. Methods:Making passive induced rat model of sensitive 31 were randomly divided into model group 7 rats,control group( n = 7),Yupingfeng Powder middle dose group( n = 7),Yupingfeng Powder high dose group( n = 7),and another 3 only as a source of serum were given service physiological saline(10 mL ·kg~(-1)),acetic acid throwing prednisone tablets in aqueous solution(2. 5 mg·kg~(-1)) and Yupingfeng Powder particles in aqueous solution(1. 35 g·kg~(-1) in the dose group,high dose group 4. 05 g·kg~(-1)) for 3 days. In this study,the rats were assaulted by the antigen. Then the Evans blue was injected in to rats tail vein. The effusion size( diameter) of the Evans blue from the skin and splenic index were measured to evaluate the immune reaction. Results:After three days of intragastric administration. Compared with the model group,middle dose group and high dose group Yupingfeng Powder made the size of the blue stain in the back of rats was decreased. The inhibition rate of middle dose group 1∶5 diluted to 11. 87%,high dose group 1∶5 diluted to 13. 76%( P < 0. 05) and 1∶10 diluted serum were reduced,but no significant difference( The inhibition rate of middle dose group 9. 80%,high dose group 11. 54%,P > 0. 05). The index of spleen index in the model group was 55. 72 ± 14. 00,and the prednisone acetate group was 37. 44 ± 9. 65. The difference between the two groups was statistically significant( P < 0. 05),and the large dose group in Yupingfeng Powder was 44. 04 ± 11. 04,and compared with the model group,the difference was not statistically significant( P > 0. 05). Conclusion:Yupingfeng Powder in the treatment of allergic skin diseases caused by type I allergic reaction has good anti allergic effect,and no obvious side effect.
引文
[1]马兰.气血辨证理论在皮肤病中的应用[J].中医学报,2013,28(1):116-119.
[2]桑红灵,李云海.田玉美教授辨治皮肤病的临床经验[J].中医学报,2012,27(3):301-302.
[3]李义奎.中药药理实验方法学[M].2版.上海:上海科学技术出版社,2006:874-875.
[4]文洁,朱建梅,李婕,等.玉屏风颗粒治疗过敏性鼻炎的实验研究[J].中成药,2011,33(6):934-937.
[5]舒小妹,曾庆祥.曾庆祥治疗过敏性皮肤病临床经验[J].中国中医药信息杂志,2014,21(11):112-114.
[6]洪敏,周红华,王亮,等.玉屏风散乙酸乙酯萃取物抑制迟发型超敏反应效应及成分分析[J].中国实验方剂学杂志,2010,16(1):71-73.
[7]谢鸣.中医方剂现代研究(上卷)[M].北京:学苑出版社,1997:783-784.
[8]王军伟.玉屏风散加减治疗顽固性荨麻疹体会[J].北京中医药,2014,33(1):38.
[9]孔丹暘.加味玉屏风散治疗慢性荨麻疹64例[J].河南中医,2015,35(2):359-361.
[10]温爱平,曾萍,谢颖,等.玉屏风颗粒治疗儿童过敏性紫癜疗效观察及对免疫球蛋白的影响[J].新中医,2014,46(6):150-151.
[11]戴前梅,胡春艳,陈朋,等.玉屏风胶囊联合枸地氯雷他定治疗慢性湿疹45例[J].河南中医,2015,35(12):3082-3084.
[12]韩莉.玉屏风散配合西药治疗慢性湿疹60例[J].陕西中医,2015,36(3):322-324.
[13]李彩霞,曾星,黄羽,等.猪苓及猪苓多糖对BBN诱导的膀胱癌大鼠胸腺、脾脏指数及CD86表达的影响[J].免疫学杂志,2012,28(2):116-119.
[14]张红霞,陈晓风.中西医结合治疗狼疮性肾炎临床研究[J].中医学报,2012,27(1):116-119.
[15]薛天萍,杨雪源.玉屏风散在皮肤科疾病治疗中的应用[J].湖南中医杂志,2014,30(1):109-110.
[16]林颖.陈达灿教授运用玉屏风散治疗疑难皮肤病经验介绍[J].新中医,2005,37(2):15-16.
[2]桑红灵,李云海.田玉美教授辨治皮肤病的临床经验[J].中医学报,2012,27(3):301-302.
[3]李义奎.中药药理实验方法学[M].2版.上海:上海科学技术出版社,2006:874-875.
[4]文洁,朱建梅,李婕,等.玉屏风颗粒治疗过敏性鼻炎的实验研究[J].中成药,2011,33(6):934-937.
[5]舒小妹,曾庆祥.曾庆祥治疗过敏性皮肤病临床经验[J].中国中医药信息杂志,2014,21(11):112-114.
[6]洪敏,周红华,王亮,等.玉屏风散乙酸乙酯萃取物抑制迟发型超敏反应效应及成分分析[J].中国实验方剂学杂志,2010,16(1):71-73.
[7]谢鸣.中医方剂现代研究(上卷)[M].北京:学苑出版社,1997:783-784.
[8]王军伟.玉屏风散加减治疗顽固性荨麻疹体会[J].北京中医药,2014,33(1):38.
[9]孔丹暘.加味玉屏风散治疗慢性荨麻疹64例[J].河南中医,2015,35(2):359-361.
[10]温爱平,曾萍,谢颖,等.玉屏风颗粒治疗儿童过敏性紫癜疗效观察及对免疫球蛋白的影响[J].新中医,2014,46(6):150-151.
[11]戴前梅,胡春艳,陈朋,等.玉屏风胶囊联合枸地氯雷他定治疗慢性湿疹45例[J].河南中医,2015,35(12):3082-3084.
[12]韩莉.玉屏风散配合西药治疗慢性湿疹60例[J].陕西中医,2015,36(3):322-324.
[13]李彩霞,曾星,黄羽,等.猪苓及猪苓多糖对BBN诱导的膀胱癌大鼠胸腺、脾脏指数及CD86表达的影响[J].免疫学杂志,2012,28(2):116-119.
[14]张红霞,陈晓风.中西医结合治疗狼疮性肾炎临床研究[J].中医学报,2012,27(1):116-119.
[15]薛天萍,杨雪源.玉屏风散在皮肤科疾病治疗中的应用[J].湖南中医杂志,2014,30(1):109-110.
[16]林颖.陈达灿教授运用玉屏风散治疗疑难皮肤病经验介绍[J].新中医,2005,37(2):15-16.