实验动物冰冻组织切片制备关键要点的探讨
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摘要
目的探讨实验动物组织冰冻切片制备的关键要点和注意事项,以提高切片质量。方法采用美国赛默飞世尔恒温式冷冻切片机,对30例SD大鼠和30例食蟹猴的皮肤、肌肉、乳腺、卵巢、子宫、胃、大肠、甲状腺、肝脏、肾脏、脾脏、淋巴结、脑、脂肪等新鲜组织进行常规冰冻切片取材、包埋、切片、染色后封片。结果镜下检查组织切面平整,细胞形态完整、清晰,染色良好。结论不同组织需要区别对待,制作优良的冰冻切片就需要对取材、组织速冻、温度选择、组织包埋、切片、固定等多个环节进行严格谨慎地操作。
Objective To improve the quality of slices,we explore the key points and consideration of freezing sectioning of experiment animals.Methods A total 30 SD tats and 30 cynomolgus monkey specimens of skin,muscle,mammary gland,ovary,uterus,stomach,intestine,thyroid,liver,kidney,spleen,lymph node,brain and fat,etc.were trimmed,embedded,sectioned and staining for routine freezing sections by Thermo low temperature cryostat microtome.Results Under microscope,the sections were smooth,and the cell morphology was complete,clear and well stained.Conclusion The different tissues need to be treated differently.To make fine frozen sections,it is necessary to operate strictly and carefully,including tissues preparation,quick freezing,selection of temperature,embedding,slicing and fixing.
Objective To improve the quality of slices,we explore the key points and consideration of freezing sectioning of experiment animals.Methods A total 30 SD tats and 30 cynomolgus monkey specimens of skin,muscle,mammary gland,ovary,uterus,stomach,intestine,thyroid,liver,kidney,spleen,lymph node,brain and fat,etc.were trimmed,embedded,sectioned and staining for routine freezing sections by Thermo low temperature cryostat microtome.Results Under microscope,the sections were smooth,and the cell morphology was complete,clear and well stained.Conclusion The different tissues need to be treated differently.To make fine frozen sections,it is necessary to operate strictly and carefully,including tissues preparation,quick freezing,selection of temperature,embedding,slicing and fixing.
引文
[1]吴艳霞.冰冻切片的制作方法分析[J].吉林医学, 2009,30(6):493-494.
[2]包翠芬,刘霞,穆长征,等.比较三种防冰晶法对切片保存效果的影响[J].中国组织化学与细胞化学杂志,2007, 16(1):122-123.
[3]杨秀静,滕孝静.冰冻切片技术经验探讨[J].临床和实验医学杂志, 2014, 13(23):2002-2004.
[4]寇雪梅,赵玉莹,朱卫国,等. Leica CM1900低温恒冷冰冻切片机的试用体会[J].黑龙江医学, 2014, 38(7):804-805.
[5]许平,王筱璨,陈奎生,等.快速冰冻切片制备方法的改进[J].郑州大学学报:医学版, 2010, 45(2):241-242.
[6]汪育苗.术中快速冰冻切片的制作方法及体会[J].北京口腔医学, 2011, 19(1):52-53.
[7]李慧,黄景阳,袁中瑞.提高脑组织冰冻切片质量的几点体会[J].中国免疫学杂志, 2012, 28(11):1019-1031.
[2]包翠芬,刘霞,穆长征,等.比较三种防冰晶法对切片保存效果的影响[J].中国组织化学与细胞化学杂志,2007, 16(1):122-123.
[3]杨秀静,滕孝静.冰冻切片技术经验探讨[J].临床和实验医学杂志, 2014, 13(23):2002-2004.
[4]寇雪梅,赵玉莹,朱卫国,等. Leica CM1900低温恒冷冰冻切片机的试用体会[J].黑龙江医学, 2014, 38(7):804-805.
[5]许平,王筱璨,陈奎生,等.快速冰冻切片制备方法的改进[J].郑州大学学报:医学版, 2010, 45(2):241-242.
[6]汪育苗.术中快速冰冻切片的制作方法及体会[J].北京口腔医学, 2011, 19(1):52-53.
[7]李慧,黄景阳,袁中瑞.提高脑组织冰冻切片质量的几点体会[J].中国免疫学杂志, 2012, 28(11):1019-1031.