野山参和园参对术后疲劳综合征大鼠骨骼肌炎症反应的影响
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摘要
目的探讨野山参和园参对术后疲劳综合征鼠骨骼肌炎症反应的作用。方法 48只大鼠随机分为对照组、模型组、园参组和野山参组,每组12只。术后1、3 d,抓力试验检测骨骼肌功能;术后3 d,荧光RT-PCR法检测促炎因子mRNA水平,Western blot法检测腓肠肌炎症和萎缩相关蛋白表达,HE染色法观察骨骼肌形态。结果术后3 d与模型组比较,园参组大鼠最大抓力显著增强(P<0.05),腓肠肌IL-1β、TNF-α mRNA表达及p-p38、p-NF-κB、atrogin-1、Murf-1蛋白表达显著降低(P<0.05);与模型组比较,野山参组大鼠最大抓力显著增强(P<0.05),腓肠肌IL-1β、TNF-α mRNA及p-p38、p-NF-κB、atrogin-1、Murf-1蛋白表达显著降低(P<0.05);与园参组比较,野山参组大鼠最大抓力增强(P<0.05),腓肠肌TNF-α mRNA表达及Murf-1蛋白表达显著降低(P<0.05)。HE染色结果显示,对照组肌纤维束直径较宽,肌束间隙紧密;模型组肌纤维束直径较小,肌束间隙较宽;园参组和野山参组肌纤维束形态较模型组得到改善。结论野山参和园参改善了术后疲劳综合征大鼠的骨骼肌炎症反应,且野山参效果优于园参。
引文
[1] Christensen T,Kehlet H.Postoperative fatigue[J].World J Surg,1993,17(2):220-225.
[2] Oliveira M,Oliveira G,Souza-Talarico J,et al.Surgical oncology:evolution of postoperative fatigue and factors related to its severity[J].Clin J Oncol Nur,2016,20(1):E3-E8.
[3] 国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:8.
[4] 何绍玉,李伟,郑毅男,等.林下山参与园参中腺苷和人参皂苷含量比较分析[J].药物分析杂志,2010,30(9):1701-1706.
[5] Zhang X D,Chen B C,Dong Q T,et al.Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats[J].Scand J Gastroenterol,2011,46(11):1302-1309.
[6] Zhuang C L,Mao X Y,Liu S,et al.Ginsenoside Rb1 improves postoperative fatigue syndrome by reducing skeletal muscle oxidative stress through activation of the PI3K/Akt/Nrf2 pathway in aged rats[J].Eur J Pharmacol,2014,740:480-487.
[7] 刘舒,吕金晓,郑蓓诗.人参皂苷Rb1改善术后疲劳综合征大鼠中枢炎症反应的机制研究[J].中草药,2015,46(14):2104-2110.
[8] Christensen T,Wulff C,Fuglsang-Frederiksen A,et al.Electrical activity and arm muscle force in postoperative fatigue[J].Acta Chir Scand,1985,151(1):1-5.
[9] Witasp A,Nordfors L,Schalling M,et al.Increased expression of inflammatory pathway genes in skeletal muscle during surgery[J].Clin Nutr,2009,28(3):291-298.
[10] Varadhan K K,Constantin-Teodosiu D,Constantin D,et al.Inflammation-mediated muscle metabolic dysregulation local and remote to the site of major abdominal surgery[J].Clin Nutr,2017,5614(17):31393-31396.
[11] Kim H J,Lee H S,Chong Y H,et al.p38 Mitogen-activated protein kinase up-regulates LPS-induced NF-κB activation in the development of lung injury and RAW 264.7 macrophages[J].Toxicology,2006,225(1):36-47.
[12] Argilés J M,López-Soriano F J,Busquets S.Muscle wasting in cancer:the role of mitochondria[J].Curr Opin Clin Nutr Metab Care,2015,18(3):221-225.
[13] Shergis J L,Zhang A L,Zhou W,et al.Panax ginseng in randomised controlled trials:a systematic review[J].Phytother Res,2013,27(7):949-965.
[14] 谈善军,余震,董千铜,等.人参皂苷Rb1对术后疲劳综合征大鼠骨骼肌氧化应激的影响[J].中国中西医结合杂志,2012,32(11):1535-1538.
[2] Oliveira M,Oliveira G,Souza-Talarico J,et al.Surgical oncology:evolution of postoperative fatigue and factors related to its severity[J].Clin J Oncol Nur,2016,20(1):E3-E8.
[3] 国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:8.
[4] 何绍玉,李伟,郑毅男,等.林下山参与园参中腺苷和人参皂苷含量比较分析[J].药物分析杂志,2010,30(9):1701-1706.
[5] Zhang X D,Chen B C,Dong Q T,et al.Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats[J].Scand J Gastroenterol,2011,46(11):1302-1309.
[6] Zhuang C L,Mao X Y,Liu S,et al.Ginsenoside Rb1 improves postoperative fatigue syndrome by reducing skeletal muscle oxidative stress through activation of the PI3K/Akt/Nrf2 pathway in aged rats[J].Eur J Pharmacol,2014,740:480-487.
[7] 刘舒,吕金晓,郑蓓诗.人参皂苷Rb1改善术后疲劳综合征大鼠中枢炎症反应的机制研究[J].中草药,2015,46(14):2104-2110.
[8] Christensen T,Wulff C,Fuglsang-Frederiksen A,et al.Electrical activity and arm muscle force in postoperative fatigue[J].Acta Chir Scand,1985,151(1):1-5.
[9] Witasp A,Nordfors L,Schalling M,et al.Increased expression of inflammatory pathway genes in skeletal muscle during surgery[J].Clin Nutr,2009,28(3):291-298.
[10] Varadhan K K,Constantin-Teodosiu D,Constantin D,et al.Inflammation-mediated muscle metabolic dysregulation local and remote to the site of major abdominal surgery[J].Clin Nutr,2017,5614(17):31393-31396.
[11] Kim H J,Lee H S,Chong Y H,et al.p38 Mitogen-activated protein kinase up-regulates LPS-induced NF-κB activation in the development of lung injury and RAW 264.7 macrophages[J].Toxicology,2006,225(1):36-47.
[12] Argilés J M,López-Soriano F J,Busquets S.Muscle wasting in cancer:the role of mitochondria[J].Curr Opin Clin Nutr Metab Care,2015,18(3):221-225.
[13] Shergis J L,Zhang A L,Zhou W,et al.Panax ginseng in randomised controlled trials:a systematic review[J].Phytother Res,2013,27(7):949-965.
[14] 谈善军,余震,董千铜,等.人参皂苷Rb1对术后疲劳综合征大鼠骨骼肌氧化应激的影响[J].中国中西医结合杂志,2012,32(11):1535-1538.