川东地区汉族人群18个STR基因座遗传多态性
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摘要
目的应用直接扩增法批量检验并调查川东地区汉族人群DNA标本在18个STR基因座的遗传多态性。方法采用DNATyper15TMPlus试剂盒直接扩增和3730XL型遗传分析仪检测STR分型。随机抽取326例该地区汉族无关个体STR分型结果,并统计18个STR基因座的等位基因分布频率及其他群体遗传学参数。结果 18个STR基因座基因型的分布符合Hardy-Weinberg平衡(P>0.05),川东地区汉族人群18个STR基因座的杂合度、匹配概率、多态信息含量、个体识别概率、非父排除概率的范围分别为0.604～0.896、0.016～0.219、0.545～0.908、0.781～0.984、0.295～0.788。结论使用直接扩增法批量检验DNA数据库标本的效率高、质量好;18个STR基因座的联合应用能获得较好的系统效能,为该地区汉族人群的遗传多态性研究及应用提供数据支持。
Objective To investigate the genetic polymorphism of 18 STR loci by a high-throughput direct amplification method.Methods DNA Typer 15~(TM) Plus kit,ABI 9700 PCR and ABI3730XL genetic analyzer were used to test 326 STR profiles of unrelated Han individuals from Eastern Sichuan.The population genetic parameters were calculated by statistic software Powerstat.Results The results demonstrated that all 18 STR loci were in accordance with the distribution of Hardy-Weinberg equilibrium(P>0.05).Heterozygosity(H)ranged from 0.604―0.896 matching probability(PM)ranged from 0.016―0.219,power of discrimination(DP)ranged from 0.803-0.985,polymorphism information content(PIC)ranged from 0.781―0.984,power of exclusion(PE)ranged from 0.295―0.788.Conclusion 18 STR loci in Han population in Eastern Sichuan have higher polymorphisms.
Objective To investigate the genetic polymorphism of 18 STR loci by a high-throughput direct amplification method.Methods DNA Typer 15~(TM) Plus kit,ABI 9700 PCR and ABI3730XL genetic analyzer were used to test 326 STR profiles of unrelated Han individuals from Eastern Sichuan.The population genetic parameters were calculated by statistic software Powerstat.Results The results demonstrated that all 18 STR loci were in accordance with the distribution of Hardy-Weinberg equilibrium(P>0.05).Heterozygosity(H)ranged from 0.604―0.896 matching probability(PM)ranged from 0.016―0.219,power of discrimination(DP)ranged from 0.803-0.985,polymorphism information content(PIC)ranged from 0.781―0.984,power of exclusion(PE)ranged from 0.295―0.788.Conclusion 18 STR loci in Han population in Eastern Sichuan have higher polymorphisms.
引文
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[14]FENG Z,XIA M,BAO H,et al.Genetic polymorphisms of 18short tandem repeat loci in 3 550individuals from the Han population of Changchun,Northeast China[J].Int J Legal Med,2016,130(6):1-3.
[15]YAO J,XING J X,XUAN J F,et al.Population data of15autosomal STR loci in Chinese Han population from Jiangsu Province,Eastern China[J].Forensic Sci Int Genet,2016,14(24):112-113.
[16]XIAO C,ZHANG W,WEI T,et al.Population data of 21autosomal STR loci in Chinese Han population from Hubei province in Central China[J].Forensic Sci Int Genet,2016,32(20):e13-e14.
[2]赵兴春,叶健.法庭DNA技术的发展及展望[J].刑事技术,1998,7(5):42-46.
[3]张建,姜成涛,赵兴春,等.直接扩增法检验纸质样本的研究[J].刑事技术,2011,19(4):33-35.
[4]张建,李红卫,王乐,等.贵州黔南地区汉族人群18个STR基因座的遗传多态性[J].刑事技术,2015,21(1):84-86.
[5]WANG L,ZHAO X C,YE J,et al.Construction of a library of cloned short tandem repeat(STR)alleles as universal templates for allelic ladder preparation[J].Forensic Sci Int Genet,2014,12(9):136-143.
[6]POWER STATS.A computer program for the analysis of population statistics[Z].1999.
[7]RAYMOND M A,ROUSSET F B.Genepop(VERSION-1.2)-population-genetics software for exact tests and ecumenicism[J].J Heredity,1995,86(3):248-249.
[8]GILL P,URQUHART A,MILLICAN E,et al.A new method of STR interpretation using inferential logic-development of a criminal intelligence database[J].Int J Legal Med,1996,109(1):14-22.
[9]高波,姜富学,王成,等.Amelogenin等位基因丢失的研究现状[J].中国公共安全(学术版),2016,10(2):112-114.
[10]时永胜,胡锡阶,周单华,等.X染色体Amelogenin基因座片段扩增峰图异常1例及荧光定量分析[J].中国计划生育学杂志,2017,25(11):781-785.
[11]胡盛平.D21S11基因座三带型伴发突变的鉴定分析[J].汕头大学医学院学报,2017,21(4):200-201.
[12]姚伊人,赵兴春,白雪,等.新疆和田地区维吾尔族人群18个STR基因座遗传多态性[J].中国法医学杂志,2015,30(3):295-296.
[13]XU X.Population data of 18autosomal STR loci in the Chinese Han population from Heilongjiang Province,Northeast China[J].Forensic Sci,2017,84(12):1698-1699.
[14]FENG Z,XIA M,BAO H,et al.Genetic polymorphisms of 18short tandem repeat loci in 3 550individuals from the Han population of Changchun,Northeast China[J].Int J Legal Med,2016,130(6):1-3.
[15]YAO J,XING J X,XUAN J F,et al.Population data of15autosomal STR loci in Chinese Han population from Jiangsu Province,Eastern China[J].Forensic Sci Int Genet,2016,14(24):112-113.
[16]XIAO C,ZHANG W,WEI T,et al.Population data of 21autosomal STR loci in Chinese Han population from Hubei province in Central China[J].Forensic Sci Int Genet,2016,32(20):e13-e14.