脱氮硫杆菌的筛选及其对锶离子的矿化作用
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摘要
放射性污染日益严重,其中锶污染作为土壤典型污染之一成为研究热点。土壤中存在着一些矿化菌,能够对锶离子进行矿化固定。本实验对从土壤中分离的3株脱氮硫杆菌的特性及其对Sr~(2+)的矿化行为进行了研究,发现该菌对1.0g/L模拟Sr~(2+)污染的去除率可达80%。扫描电子显微镜(SEM)、能谱分析(EDS)、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)等结果显示,矿化产物为硫酸锶。可见,利用脱氮硫杆菌治理土壤中Sr~(2+)污染具有可行性,该方法将会有一定应用前景。
Radioactive pollution is increasingly serious,and strontium contamination is a research hot point as one of the typical radioactive pollution.There are some mineralization bacteria in soil which can immobilize the heavy metal ion in the soil.3strains was isolated from soil and its characterization and mineralization to Sr~(2+)was carried on.Results show that the removal rate of strains to 1.0g/L simulated Sr~(2+)is up to 80%.Scanning electron microscope(SEM),energy spectrum analysis(EDS),X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR)results show that mineral products is strontium sulfate.This study demonstrate that using Thiobacillus denitrificans for Sr~(2+)pollution removal in the soil is feasible,and the method will have some application prospect.
Radioactive pollution is increasingly serious,and strontium contamination is a research hot point as one of the typical radioactive pollution.There are some mineralization bacteria in soil which can immobilize the heavy metal ion in the soil.3strains was isolated from soil and its characterization and mineralization to Sr~(2+)was carried on.Results show that the removal rate of strains to 1.0g/L simulated Sr~(2+)is up to 80%.Scanning electron microscope(SEM),energy spectrum analysis(EDS),X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR)results show that mineral products is strontium sulfate.This study demonstrate that using Thiobacillus denitrificans for Sr~(2+)pollution removal in the soil is feasible,and the method will have some application prospect.
引文
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[2]Falciglia P P,Cannata S,Romano S,et al.Stabilisation/solidification of radionuclide polluted soils:partⅠ:assessment of setting time,mechanical resistance,γ-radiation shielding and leachateγ-radiation[J].J Geochem Explor,2014,142:104-111.
[3]Wang D,Wen F,Xu C,et al.The uptake of Cs and Sr from soil to radish(Raphanus sativus L.)-potential for phytoextraction and remediation of contaminated soils[J].J Environ Radioact,2012,110:78-83.
[4]Govarthanan M,Lee K J,Cho M,et al.Significance of autochthonous Bacillus sp.KK1on biomineralization of lead in mine tailings[J].Chemosphere,2012,90(8):2267-2272.
[5]Kim G N,Shon D B,Park H M,et al.Development of pilot-scale electrokinetic remediationtechnology for uranium removal[J].Sep Purif Technol,2011,80(1):67-72.
[6]Addadi L,Raz S,Weiner S.Taking advantage of disorder:amorphous calcium carbonate and its roles in bio-mineralization[J].Adv Mater,2003,15:959-970.
[7]竹文坤,牟涛,段涛,等.碳酸盐矿化菌对模拟放射性Sr2+的成矿作用[J].环境科学研究,2015,28(1):157-162.
[8]Li M,Cheng X H,Guo H X.Heavy metal removal by biomineralization of urease producing bacteria isolated from soil[J].International Biodeterioration&Biodegradation,2013,76:81-85.
[9]许凤琴,代群威,侯丽华,等.碳酸盐矿化菌的分纯及其对Sr2+的矿化特性研究[J].高校地质学报,2015,21(3):376-381.
[10]徐卫华,刘云国,曾光明,等.硫酸盐还原菌及其还原解毒Cr(Ⅵ)的研究进展[J].微生物学通报,2009,36(7):1040-1045.
[11]王婷.蜡状芽孢杆菌修复重金属及多溴联苯醚复合污染的研究[D].广州:暨南大学,2007.
[12]刘明学,董发勤,李姝,等.固定化耐辐射奇球菌对锶柱吸附与减量化研究[J].环境科学与技术,2014,6:32-37.
[13]Buchan R E,Gibbens N E.中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组译.伯杰细菌鉴定手册[M].第八版.北京:科学出版社,1984:643-650.
[14]车轩,罗国芝,谭洪新,等.脱氮硫杆菌的分离鉴定和反硝化特性研究[J].环境科学,2008,29(10):2931-2937.
[15]Bisogni J J,Driscoll C T.Dentirfication using thiosulfate and sulfide[J].J Environ Eng Div Am Soc Civ Eng,1977,1039(10):593-604.
[16]韩桂琪,王彬,徐卫红,等.重金属Cd、Zn、Cu、Pb复合污染对土壤微生物和酶活性的影响[J].水土保持学报,2010,24(5):238-242.
[17]李淑英,马玉琪,苏亚丽,等.重金属胁迫培养对微生物生长的影响[J].贵州农业科学,2012,40(2):90-94.