北京地区老年人外周血淋巴细胞亚群正常参考范围的建立及比较
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摘要
目的建立及比较北京地区不同年龄段老年人外周血淋巴细胞亚群正常参考范围。方法将参与2018年度解放军总医院健康体检的524例北京地区健康成人按10岁为1年龄段共分为4组,采用美国Beckman流式细胞仪结合血液分析仪分别检测他们的外周血CD4~+、CD3~+及CD8~+等T淋巴亚群及CD19~+及NK细胞,采用SPSS 17.0软件来分别比较各组间各项指标的统计学差异,并采用受检人群中年龄≥60岁的老年人的检测结果来获得适用于北京地区老年人的淋巴细胞亚群正常参考范围。结果淋巴细胞百分比随年龄的增长有所降低; CD3~+、CD3~+CD8~+及CD4~+45RO~+占淋巴细胞百分比随着年龄的增长而增高;自然杀伤细胞(NK)占淋巴细胞百分比随着年龄的增长而降低。北京地区老年人外周血淋巴细胞亚群正常参考范围(以95%CI表示)如下:淋巴细胞:759～2 783 cell/μl(12.9～47.3);CD3~+:929～1610 cell/μl(53.08～91.92); CD3~+CD8~+:202～775 cell/μl(11.53～44.25); CD3~+CD4~+:250～1248 cell/μl(14.30～71.30); CD4~+45RA~+:33～662 cell/μl(1.9～37.80); CD4~+45RO~+:199～807 cell/μl(11.34～46.07); CD19:7～403 cell/μl(0.40～22.99); NK:32～501 cell/μl(1.80～28.61)。结论年龄因素可影响人外周血淋巴细胞亚群CD3~+、CD3~+CD8~+、CD4~+45RO~+及NK占淋巴细胞百分比,有必要建立适当的、适用于北京地区老年人的上述常见淋巴细胞亚群正常参考范围。
Objective To establish and compare the referential range of peripheral blood lymphocyte subsets in elderly inhabitants with different age ranges in Beijing area.Methods 524 healthy adults participate in the deppartment of physical examination were divided into four groups according to their age ranges.Their peripheral blood CD4~+,CD3~+and CD8~+T lymphoid subgroups and CD19~+ and NK cells were detected by American Beckman flow cytometry and hematology analysis.SPSS 17.0 was used to compare the statistical difference between these various indexes in each group.The referential ranges of lymphocyte subsets suitable for healthy elderly persons in Beijing were obtained from the test results of elderly persons over 60 years of age.Results The percentage of total lymphocytes is decreased with age range; CD3~+%,CD3~+CD8~+% and CD4~+45 RO~+% have increased with age range; NK% have decreased with the increase of age range.The referential range of peripheral blood lymphocyte subsets of healthy elderly persons in Beijing(expressed in 95% confidential intervals) is as follows: lymphocytes: 759 ～ 2783 cells/μl(12.90% ～ 47.3%); CD3~+: 929 ～ 1610 cells/μl(53.08% ～ 91.92%); CD3~+CD8~+: 202 ～ 775 cells/μl(11.53% ～ 44.25%);CD3~+CD4~+: 250 ～ 1248 cells/μl(14.30% ～ 71.30%); CD4~+45 RA~+: 33 ～ 662 cells/μl(1.90% ～ 37.80%); CD4~+45 RO~+: 199 ～807 cells/μl(11.34% ～ 46.07%); CD19: 7 ～ 403 cells/μl(0.40% ～ 22.99 %); NK: 32 ～ 501 cells/μl(1.80% ～ 28.61%).Conclusion Age factors may influence the percentage of lymphocyte subsets CD3~+,CD3~+CD8~+,CD4~+45 RO~+and NK~+in human peripheral blood.It is necessary to establish the normal referential range of these common lymphocyte subsets suitable for the elderly inhabitants in Beijing area.
Objective To establish and compare the referential range of peripheral blood lymphocyte subsets in elderly inhabitants with different age ranges in Beijing area.Methods 524 healthy adults participate in the deppartment of physical examination were divided into four groups according to their age ranges.Their peripheral blood CD4~+,CD3~+and CD8~+T lymphoid subgroups and CD19~+ and NK cells were detected by American Beckman flow cytometry and hematology analysis.SPSS 17.0 was used to compare the statistical difference between these various indexes in each group.The referential ranges of lymphocyte subsets suitable for healthy elderly persons in Beijing were obtained from the test results of elderly persons over 60 years of age.Results The percentage of total lymphocytes is decreased with age range; CD3~+%,CD3~+CD8~+% and CD4~+45 RO~+% have increased with age range; NK% have decreased with the increase of age range.The referential range of peripheral blood lymphocyte subsets of healthy elderly persons in Beijing(expressed in 95% confidential intervals) is as follows: lymphocytes: 759 ～ 2783 cells/μl(12.90% ～ 47.3%); CD3~+: 929 ～ 1610 cells/μl(53.08% ～ 91.92%); CD3~+CD8~+: 202 ～ 775 cells/μl(11.53% ～ 44.25%);CD3~+CD4~+: 250 ～ 1248 cells/μl(14.30% ～ 71.30%); CD4~+45 RA~+: 33 ～ 662 cells/μl(1.90% ～ 37.80%); CD4~+45 RO~+: 199 ～807 cells/μl(11.34% ～ 46.07%); CD19: 7 ～ 403 cells/μl(0.40% ～ 22.99 %); NK: 32 ～ 501 cells/μl(1.80% ～ 28.61%).Conclusion Age factors may influence the percentage of lymphocyte subsets CD3~+,CD3~+CD8~+,CD4~+45 RO~+and NK~+in human peripheral blood.It is necessary to establish the normal referential range of these common lymphocyte subsets suitable for the elderly inhabitants in Beijing area.
引文
[1]赵雯,张子宁,王亚男,等.2 862例临床患者T淋巴细胞亚群检测结果分析[J].中国医科大学学报,2012,41(11):1026-1029.
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[3]Deneys V,Mazzon AM,Marques JL,et al.Reference values for peripheral blood B-lymphocyte subpopulations:a basis for multiparametric immunophenotyping of abnormal lymphocytes[J].J Immunol Methods,2001,253(1-2):23-36.
[4]董静懿.健康成年人外周血T淋巴细胞亚群参考范围调查[A].中国中西医结合学会.第一次全国中西医结合检验医学学术会议暨中国中西医结合学会检验医学专业委员会成立大会论文汇编[C].中国中西医结合学会,2014:1.
[5]许遵鹏,李蓓,廖灿,等.广州市健康儿童和成人淋巴细胞亚群数量的对比分析[J].中国优生与遗传杂志,2012,20(2):126-128,125.
[6]刘帅凤,梁富雄,沈智勇,等.建立中国广西壮族健康人群外周血T淋巴细胞亚群正常范围值:性别、年龄和民族影响因素的分析[J].中华微生物学和免疫学杂志,2016,36(5):374-379.
[7]郭志宏,姚亚萍,李西婷,等.浙江地区健康青少年和成人外周血T淋巴细胞免疫表型调查及正常参考值范围的建立[J].中国预防医学杂志,2008,9(1):32-35.
[8]孙大菊,刘颖男,吕惠影,等.长春地区120例健康成人外周血T淋巴细胞亚群的参考范围[J].中国实验诊断学,2017,21(12):2160-2161.
[9]张灿强,何继宏,张芳娣,等.不同T淋巴细胞亚群在恶性肿瘤患者外周血中的临床检测价值[J].湖北科技学院学报(医学版),2017,31(5):381-383.
[10]苏丽.恶性肿瘤患者外周血中T淋巴细胞亚群及免疫球蛋白水平检测价值[J].安徽医药,2018,22(7):1354-1356.
[11]李鸣.白细胞共同分化抗原CD45[J].国外医学(分子生物学分册),1995,(3):112-116.
[12]方广,黄韧.天然杀伤细胞在肿瘤免疫治疗中的研究进展[J/OL].中国实验动物学报:1-10[2018-10-14].http://kns.cnki.net/kcms/detail/11.2986.Q.20180920.0913.004.html.
[2]吴士及,徐丽娟,黄劲,等.健康成年人和儿童外周血淋巴细胞亚群参考区间的建立[J].国际检验医学杂志,2017,38(5):593-595.
[3]Deneys V,Mazzon AM,Marques JL,et al.Reference values for peripheral blood B-lymphocyte subpopulations:a basis for multiparametric immunophenotyping of abnormal lymphocytes[J].J Immunol Methods,2001,253(1-2):23-36.
[4]董静懿.健康成年人外周血T淋巴细胞亚群参考范围调查[A].中国中西医结合学会.第一次全国中西医结合检验医学学术会议暨中国中西医结合学会检验医学专业委员会成立大会论文汇编[C].中国中西医结合学会,2014:1.
[5]许遵鹏,李蓓,廖灿,等.广州市健康儿童和成人淋巴细胞亚群数量的对比分析[J].中国优生与遗传杂志,2012,20(2):126-128,125.
[6]刘帅凤,梁富雄,沈智勇,等.建立中国广西壮族健康人群外周血T淋巴细胞亚群正常范围值:性别、年龄和民族影响因素的分析[J].中华微生物学和免疫学杂志,2016,36(5):374-379.
[7]郭志宏,姚亚萍,李西婷,等.浙江地区健康青少年和成人外周血T淋巴细胞免疫表型调查及正常参考值范围的建立[J].中国预防医学杂志,2008,9(1):32-35.
[8]孙大菊,刘颖男,吕惠影,等.长春地区120例健康成人外周血T淋巴细胞亚群的参考范围[J].中国实验诊断学,2017,21(12):2160-2161.
[9]张灿强,何继宏,张芳娣,等.不同T淋巴细胞亚群在恶性肿瘤患者外周血中的临床检测价值[J].湖北科技学院学报(医学版),2017,31(5):381-383.
[10]苏丽.恶性肿瘤患者外周血中T淋巴细胞亚群及免疫球蛋白水平检测价值[J].安徽医药,2018,22(7):1354-1356.
[11]李鸣.白细胞共同分化抗原CD45[J].国外医学(分子生物学分册),1995,(3):112-116.
[12]方广,黄韧.天然杀伤细胞在肿瘤免疫治疗中的研究进展[J/OL].中国实验动物学报:1-10[2018-10-14].http://kns.cnki.net/kcms/detail/11.2986.Q.20180920.0913.004.html.