化学发光法与酶联免疫吸附测定法在乙肝两对半检测中的应用
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摘要
目的分析化学发光法与酶联免疫吸附测定法在乙肝两对半检测中的应用价值。方法研究对象选取为2016年1月～2018年6月本院收治的174例乙肝病毒携带者,采集患者血清标本,分别采用化学发光法与酶联免疫吸附测定法进行检测,对比两种检验结果的一致性。结果两种检验方案的多数血清学标志物模式检验结果基本一致,但化学发光法在抗HbsAg、抗HbeAg检验阳性率上为100.0%,要显著高于酶联免疫吸附试验的71.4%;从各检验标志物的一致性分析来看,两种检验方案的HBsAg、抗-HBs的一致性极强(Kappa=1.000,0.819),HBeAg、抗-HBe的一致性较强(Kappa=0.769,0.713),抗-HBc的一致性仅为中等(Kappa=0.471)。结论化学发光法与酶联免疫吸附测定法在乙肝两对半检测中的应用价值均较高,但化学发光法属于半定量检验,相对临床应用价值更高。
Objective To analyze the application value of chemiluminescence and enzyme-linked immunosorbent assay in the detection of hepatitis B. Methods A total of 174 hepatitis B virus carriers admitted to our hospital from January 2016 to June 2018 were selected. Serum samples were collected and detected by chemiluminescence and enzyme-linked immunosorbent assay(ELISA). Results Most of the serological marker patterns of the two test schemes were basically identical, but the positive rate of anti-HbsAg and anti-HbeAg test by chemiluminescence method was 100.0%, which was significantly higher than 71.4% by ELISA. The consistency of HBeAg and anti-HBe was strong(Kappa = 0.769, 0.713), and the consistency of anti-HBc was only moderate(Kappa = 0.471). Conclusion Chemiluminescence and enzyme-linked immunosorbent assay are both of high value in the detection of hepatitis B, but chemiluminescence belongs to semi-quantitative test and has higher value in clinical application.
Objective To analyze the application value of chemiluminescence and enzyme-linked immunosorbent assay in the detection of hepatitis B. Methods A total of 174 hepatitis B virus carriers admitted to our hospital from January 2016 to June 2018 were selected. Serum samples were collected and detected by chemiluminescence and enzyme-linked immunosorbent assay(ELISA). Results Most of the serological marker patterns of the two test schemes were basically identical, but the positive rate of anti-HbsAg and anti-HbeAg test by chemiluminescence method was 100.0%, which was significantly higher than 71.4% by ELISA. The consistency of HBeAg and anti-HBe was strong(Kappa = 0.769, 0.713), and the consistency of anti-HBc was only moderate(Kappa = 0.471). Conclusion Chemiluminescence and enzyme-linked immunosorbent assay are both of high value in the detection of hepatitis B, but chemiluminescence belongs to semi-quantitative test and has higher value in clinical application.
引文
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[3]杜清标.实时荧光PCR检测乙肝病毒DNA的临床意义探析[J].医学信息,2015(45):73-74.
[4]王春翠,何素梅.实时荧光定量PCR检测HBV-DNA临床指导意义的研究[J].临床医药文献电子杂志,2014,1(9):1489-1490.
[5]王金梅.探讨实时荧光PCR法检测乙肝病毒DNA在临床中的应用[J].中国医药指南,2013,19(25):173.