釉基质衍生物改性后的牙本质表面对人牙周膜干细胞的影响
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摘要
目的:研究釉基质衍生物(EMD)改性后的牙本质支架表面对人牙周膜干细胞(PDLSCs)的生物学影响。方法:分离培养人PDLSCs;构建不同浓度EMD加载的牙本质支架表面;DAPI染色和CCK-8检测各组复合支架上PDLSCs的黏附和增殖活性以及细胞骨架伸展能力;q-PCR检测细胞成骨及成牙骨质相关基因的表达。结果:加载EMD组牙本质支架上的PDLSCs黏附、增殖能力增强,细胞伸展能力更强,成骨及成牙骨质相关基因表达升高,且存在一定浓度依赖效应。结论:EMD改性的牙本质支架可以促进PDLSCs的黏附、增殖、细胞伸展和成骨、成牙骨质向分化。
Objective: To study the biological effects of enamel matrix derivative(EMD) modified dentin matrix on human periodontal stem cells(PDLSCs). Methods: PDLSCs were isolated and cultured. The treated dentin matrix was loaded with EMD at different concentrations. PDLSCs adhesion and proliferation on the scaffold were observed by DAPI staining and CCK-8 kit, osteogenesis and cementogenesis related genes were studied by q-PCR. Results: The proliferation activity of PDLSCs on the EMD group was higher, the adhesion and extension were stronger, and the expression of bone and cement-related genes was higher, and EMD showed a dose-dependent promotion effects. Conclusion: EMD modified dentin matrix can promote the proliferation, adhesion, extension and osteogenic-cementogenic differentiation of PDLSCs.
Objective: To study the biological effects of enamel matrix derivative(EMD) modified dentin matrix on human periodontal stem cells(PDLSCs). Methods: PDLSCs were isolated and cultured. The treated dentin matrix was loaded with EMD at different concentrations. PDLSCs adhesion and proliferation on the scaffold were observed by DAPI staining and CCK-8 kit, osteogenesis and cementogenesis related genes were studied by q-PCR. Results: The proliferation activity of PDLSCs on the EMD group was higher, the adhesion and extension were stronger, and the expression of bone and cement-related genes was higher, and EMD showed a dose-dependent promotion effects. Conclusion: EMD modified dentin matrix can promote the proliferation, adhesion, extension and osteogenic-cementogenic differentiation of PDLSCs.
引文
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[2] Amin HD,Olsen I,Knowles J et al.A tyrosine- rich amelogenin peptide promotes neovasculogenesis in vitro and ex vivo[J].Acta Biomater,2014,10(5):1930-1939.
[3] Stout BM,Alent BJ,Pedalino P,et al.Enamel matrix derivative:Protein components and osteoinductive properties[J].J Periodontol,2014,85(2):e9-e17.
[4] Lyngstadaas SP,Wohlfahrt JC,Brookes SJ,et al.Enamel matrix proteins;old molecules for new applications[J].Orthod Craniofac Res,2009,12(3):243-253.
[5] Gassmann G,Schwenk B,Entschladen F,et al.Influence of enamel matrix derivative on primary CD4+ T- helper lymphocyte migration,CD25 activation,and apoptosis[J].J Periodontol,2009,80(9):1524-1533.
[6] Arweiler NB,Auschill TM,Donos N,et al.Antibacterial effect of an enamel matrix protein derivative on in vivo dental biofilm vitality[J].Clin Oral Investig,2002,6(4):205-209.
[7] Walter C,Jawor P,Bernimoulin JP,et al.Moderate effect of enamel matrix derivative (Emdogain Gel) on Porphyromonas gingivalis growth in vitro[J].Arch Oral Biol,2006,51(3):171-176.
[8] Thoma DS,Villar CC,Carnes DL,et al.Angiogenic activity of an enamel matrix derivative (EMD) and EMD- derived proteins:An experimental study in mice[J].J Clin Periodontol,2011,38(3):253-260.
[9] Kauvar AS,Thoma DS,Carnes DL,et al.In vivo angiogenic activity of enamel matrix derivative[J].J Periodontol,2010,81(8):1196-1201.
[10] Sculean A,Chiantella GC,Windisch P,et al.Clinical and histologic evaluation of human intrabony defects treated with an enamel matrix protein derivative (Emdogain)[J].Int J Periodontics Restorative Dent,2000,20(4):374-381.
[11] 张森林,毛天球,孟昭业,等.重组人骨形成蛋白- 2和牛骨形成蛋白骨诱导活性的比较研究[J].华西口腔医学杂志,1998,16(3):209-210.
[12] 许杰,邓再喜,马志伟,等.应用引导组织再生术修复外伤后牙槽骨缺损的临床研究[J].实用口腔医学杂志,2011,27(5):699-702.