金花茶花朵和叶片UPLC-QTOF-MS分析
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摘要
[目的]鉴定分析金花茶花朵和叶片中主要化学成分、含量及其变化特征,为金花茶资源的进一步开发利用提供科学依据。[方法]利用超高效液相色谱-飞行时间质谱联用(UPLC-QTOF-MS)技术定性定量分析金花茶花瓣、雄蕊、老叶和新叶中花青苷、类黄酮及儿茶素类成分与含量。[结果]花青苷天竺葵素-3-O-葡萄糖苷和矢车菊素-3-O-葡萄糖苷均为金花茶中首次发现,其中,矢车菊素-3-O-葡萄糖苷仅存在于紫红色新叶中。类黄酮木犀草素-7-O-芸香糖苷和染料木苷为金花茶中首次发现,槲皮素-3-O-葡萄糖苷、槲皮素-7-O-葡萄糖苷、槲皮素-3-O-芸香糖苷和山柰酚-3-O-葡萄糖苷为金花茶叶片中首次发现。金花茶花瓣与雄蕊中花青苷相差不大,但却低于叶片尤其新叶;花朵中儿茶素类远高于叶片尤其新叶。金花茶花瓣和雄蕊中总黄酮及槲皮素-3-O-葡萄糖苷、槲皮素-3-O-芸香糖苷和山柰酚-3-O-葡萄糖苷均相差不大,但却远高于叶片。金花茶新叶中主要类黄酮成分木犀草素-7-O-芸香糖苷及总类黄酮明显高于老叶。[结论]金花茶中共鉴定出2种花青苷、6种类黄酮和2种儿茶素;槲皮素-3-O-葡萄糖苷等类黄酮是金花茶花朵呈现黄色的主要原因,矢车菊素-3-O-葡萄糖苷是金花茶新叶呈现紫红色的主要原因。
[Objective] To identify and analyze the main chemical components,their contents and change in the flowers and leaves of Camellia nitidissima,and to provide the basis for further exploitation and utilization of C. nitidissima. [Method] The components and contents of anthocyanins,flavonoids and catechins in the flowers and leaves of C. nitidissima were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry. [Result]Cyanidin-3-O-glucoside and pelargonium-3-O-glucoside were identified in C. nitidissima for the first time,and cyanidin-3-O-glucoside was only found in the new purple leaves.Luteolin-7-O-rutinoside and genistin were identified in C. nitidissima for the first time,and the quercetin-3-O-glucoside,quercetin-7-O-glucoside,quercetin-3-O-rutinoside and kaempferol-3-O-glucoside were found in leaves of C.nitidissima for the first time. The contents of anthocyanins between petals and stamens had no obvious difference,but were lower than that in leaves,especially new leaves. Instead,the contents of catechins in flowers were well higher than that in leaves,especially new leaves. The contents of total flavonoids,quercetin-3-O-glucoside,quercetin-3-O-rutinoside and kaempferol-3-O-glucoside between petals and stamens had no obvious differences,but werefar higher than that in leaves. The contents of total flavonoids and luteolin-7-O-rutinoside which was the main flavonoids in new leaves were significantly higher than that in old leaves. [Conclusion] Two anthocyanins,six flavonoids and two catechins are identified in C. nitidissima. The quercetin-3-O-glucoside and other flavonoids are the main cause of the yellow color of flowers,and the cyanidin-3-o-glucoside is the main cause of the purple color of new leaves of C. nitidissima.
[Objective] To identify and analyze the main chemical components,their contents and change in the flowers and leaves of Camellia nitidissima,and to provide the basis for further exploitation and utilization of C. nitidissima. [Method] The components and contents of anthocyanins,flavonoids and catechins in the flowers and leaves of C. nitidissima were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry. [Result]Cyanidin-3-O-glucoside and pelargonium-3-O-glucoside were identified in C. nitidissima for the first time,and cyanidin-3-O-glucoside was only found in the new purple leaves.Luteolin-7-O-rutinoside and genistin were identified in C. nitidissima for the first time,and the quercetin-3-O-glucoside,quercetin-7-O-glucoside,quercetin-3-O-rutinoside and kaempferol-3-O-glucoside were found in leaves of C.nitidissima for the first time. The contents of anthocyanins between petals and stamens had no obvious difference,but were lower than that in leaves,especially new leaves. Instead,the contents of catechins in flowers were well higher than that in leaves,especially new leaves. The contents of total flavonoids,quercetin-3-O-glucoside,quercetin-3-O-rutinoside and kaempferol-3-O-glucoside between petals and stamens had no obvious differences,but werefar higher than that in leaves. The contents of total flavonoids and luteolin-7-O-rutinoside which was the main flavonoids in new leaves were significantly higher than that in old leaves. [Conclusion] Two anthocyanins,six flavonoids and two catechins are identified in C. nitidissima. The quercetin-3-O-glucoside and other flavonoids are the main cause of the yellow color of flowers,and the cyanidin-3-o-glucoside is the main cause of the purple color of new leaves of C. nitidissima.
引文
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[13]应震,张晶,殷恒福,等.茶花红叶芽变品种‘金华美女’叶色突变相关主要化学成分含量变化[J].园艺学报,2017,44(4):723-732.
[14]龚加顺,隋华嵩,彭春秀,等.“紫娟”晒青绿茶色素的HPLCESI-MS分离鉴定及其稳定性研究[J].茶叶科学,2012,32(2):179-188.
[15]解东超,戴伟东,李朋亮,等.基于LC-MS的紫娟烘青绿茶加工过程中花青素变化规律研究[J].茶叶科学,2016,36(6):603-612.
[16]Li J B,Hashimoto F,Sakata Y. Anthocyanins from red flowers of Camellia reticulata L[J]. Bioscience Biotechnology&Biochemistry,2007,71(11):2833-2836.
[17] Li J B,Hashimoto F,Shimizu K,et al. Anthocyanins from red flowers of Camellia cultivar‘Dalicha’[J]. Phytochemistry,2008,69(18):3166-3171.
[18]Li J B,Hashimoto F,Shimizu K,et al. A new acylated anthocyan inform the red flowers of Camellia hongkongensis and characterization of anthocyanins in the Section Camellia species[J]. Journal of Plant Ecology,2009,51(6):545-552.
[19]夏星,黄嘉骏,王志萍,等.金花茶叶的降血糖作用及急性毒性研究[J].时珍国医国药,2013,24(5):1281-1282.
[20]柴胜丰,唐健民,陈宗游,等.毛瓣金花茶叶片化学成分及生理活性物质分析[J].食品科技,2016,(3):110-114.
[21]Sangwan N S,Shanker S,Sangwan R S,et al. Plant-derived products as antimutagens[J]. Phytotherapy Research,2015,12(6):389-399.
[2]Lin J N,Lin H Y,Yang N S,et al. Chemical constituents and anticancer activity of yellow Camellias against MDA-MB-231 human breast cancer cells[J]. Journal of Agricultural&Food Chemistry,2013,61(40):9638-9644.
[3]He D Y,Wang X T,Zhang P,et al. Evaluation of the anxiolytic and antidepressant activities of the aqueous extract from Camellia euphlebia Merr. ex Sealy in mice[J]. Evidence-Based Complementray and Alternative Medicine,2015,11:1-8.
[4]Song L X,Wang X S,Zheng X Q,et al. Polyphenolic antioxidant profiles of yellow Camellia[J]. Food Chemistry,2011,129(2):351-357.
[5]He D Y,Li X Y,Xuan S,et al. Camellia nitidissima,C. W. Chi:a review of botany,chemistry and pharmacology[J]. Phytochemistry Reviews,2018,17(2):327-349.
[6]Qi J,Shi R F,Yu J M,et al. Chemical constituents from leaves of Camellia nitidissima and their potential cytotoxicity on SGC7901Cells[J]. Chinese Herbal Medicines,2016,8(1):80-84.
[7]彭晓,于大永,冯宝民,等.金花茶花化学成分的研究[J].广西植物,2011,31(4):550-553.
[8]Hashimoto F,Tanaka M,Maeda H,et al. Changes in flower coloration and sepal anthocyanins of cyanic Delphinium cultivars during flowering[J]. Bioscience Biotechnology and Biochemistry,2002,66(8):1652-1659.
[9]Wang L S,Heshimoto F,Shiraishi A,et al. Chemical taxonomy in Xibei tree peony from China by floral pigmentation[J]. Journal of Plant Research,2004,117(1):47-55.
[10]张维冰,王智聪,张凌怡.超高效液相色谱-二极管阵列检测-串联质谱法测定菊花中的10种咖啡酰基奎宁酸和22种黄酮类化合物[J].分析化学,2013,41(12):1851-1861.
[11]李蓓佳,向诚,杨秀伟,等.应用高效液相色谱-质谱联用技术研究通脉颗粒的指纹图谱[J].药学学报,2010,45(11):1410-1414.
[12]Zhou X W,Fan Z Q,Chen Y,et al. Functional analyses of a flavonol synthase-like gene from Camellia nitidissima reveal its roles in flavonoid metabolism during floral pigmentation[J]. Journal of Biosciences,2013,38(3):593-604.
[13]应震,张晶,殷恒福,等.茶花红叶芽变品种‘金华美女’叶色突变相关主要化学成分含量变化[J].园艺学报,2017,44(4):723-732.
[14]龚加顺,隋华嵩,彭春秀,等.“紫娟”晒青绿茶色素的HPLCESI-MS分离鉴定及其稳定性研究[J].茶叶科学,2012,32(2):179-188.
[15]解东超,戴伟东,李朋亮,等.基于LC-MS的紫娟烘青绿茶加工过程中花青素变化规律研究[J].茶叶科学,2016,36(6):603-612.
[16]Li J B,Hashimoto F,Sakata Y. Anthocyanins from red flowers of Camellia reticulata L[J]. Bioscience Biotechnology&Biochemistry,2007,71(11):2833-2836.
[17] Li J B,Hashimoto F,Shimizu K,et al. Anthocyanins from red flowers of Camellia cultivar‘Dalicha’[J]. Phytochemistry,2008,69(18):3166-3171.
[18]Li J B,Hashimoto F,Shimizu K,et al. A new acylated anthocyan inform the red flowers of Camellia hongkongensis and characterization of anthocyanins in the Section Camellia species[J]. Journal of Plant Ecology,2009,51(6):545-552.
[19]夏星,黄嘉骏,王志萍,等.金花茶叶的降血糖作用及急性毒性研究[J].时珍国医国药,2013,24(5):1281-1282.
[20]柴胜丰,唐健民,陈宗游,等.毛瓣金花茶叶片化学成分及生理活性物质分析[J].食品科技,2016,(3):110-114.
[21]Sangwan N S,Shanker S,Sangwan R S,et al. Plant-derived products as antimutagens[J]. Phytotherapy Research,2015,12(6):389-399.