响应面法优化太空茄子叶总黄酮的提取工艺
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摘要
【目的】优化太空茄子叶中总黄酮的提取工艺.【方法】以太空茄子叶为试材,在单因素试验的基础上,取乙醇体积分数、料液比、提取时间为自变量,太空茄子叶总黄酮得率为响应值,以响应面法得到二次多项式模型,优化其提取条件,并采用HPLC对总黄酮进行分析和鉴定.【结果】在温度(80℃)固定的条件下,太空茄子叶总黄酮的最佳工艺条件为:提取时间1.8 h、料液比1∶42(g/mL)、乙醇体积分数78%,在此条件下,太空茄子叶总黄酮得率为9.82%,与预测值9.87%相吻合.【结论】响应面分析法优化太空茄子叶总黄酮的提取工艺合理可行.
【Objective】 To optimize the extraction process of total flavonoids from space eggplant leaves.【Method】 On the basis of a single factor experiment,space eggplant leaves were used as the test material,ethanol concentration,solid-to-liquid ratio,and extraction time were used as independent variables,the extraction yield of total flavonoids was used as response value.A quadratic polynomial model was established according to the response surface methodology.By optimizing the extracting condition,the total flavonoids were analyzed and identified by HPLC.【Result】 At the constant ambient temperature of 80 ℃,the optimal conditions for ethanol reflux extraction of total flavonoids were found to be as follows,extraction time 1.8 h,solid-to-liquid ratio 1∶42(g/mL),ethanol concentration 78%.Under these conditions,the extraction yield of total flavonoids was 9.82%,which was fully in agreement with the predicted value of 9.87%.【Conclusion】 It is suggested that response surface analysis to optimazation of extraction process of total flavenoids from space eggplant leaves is feasible.
【Objective】 To optimize the extraction process of total flavonoids from space eggplant leaves.【Method】 On the basis of a single factor experiment,space eggplant leaves were used as the test material,ethanol concentration,solid-to-liquid ratio,and extraction time were used as independent variables,the extraction yield of total flavonoids was used as response value.A quadratic polynomial model was established according to the response surface methodology.By optimizing the extracting condition,the total flavonoids were analyzed and identified by HPLC.【Result】 At the constant ambient temperature of 80 ℃,the optimal conditions for ethanol reflux extraction of total flavonoids were found to be as follows,extraction time 1.8 h,solid-to-liquid ratio 1∶42(g/mL),ethanol concentration 78%.Under these conditions,the extraction yield of total flavonoids was 9.82%,which was fully in agreement with the predicted value of 9.87%.【Conclusion】 It is suggested that response surface analysis to optimazation of extraction process of total flavenoids from space eggplant leaves is feasible.
引文
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[22] 罗慧玉,闫伟伟,曾春萍,等.HPLC 法同时测定杏花中阿魏酸,芦丁和异槲皮苷[J].实用药物与临床,2017,20(6):701-704.
[22] 马智超,翟春梅,怀雪,等.响应面法优化山荆子果实总黄酮的提取工艺[J].中医药学报,2018,46(5):45-49.
[23] 赵利敏,马绍英,杨海荣,等.响应面分析法优化葡萄籽原花青素的提取工艺[J].甘肃农业大学学报,2012,47(2):120-125.
[24] 王娜,王栋梁,范会平,等.不同提取方法对枣渣黄酮抗氧化活性和得率的影响[J].河南农业大学学报,2015,49(6):843-849.
[2] 代明,刘云.山东地区露地茄子种植技术探讨[J].农技服务,2016,33(5):74.
[3] 邱丹缨,罗彩林,温扬敏,等.响应面优化秋茄叶粗多糖提取及其抗肿瘤活性[J].泉州师范学院学报,2017,35(2):53-57.
[4] Dong-Ryeol B,Min-Jee L,Nam-In B,et al.Phenolic compounds from the leaves of eggplant (Solanum melongena L.)[J].Journal of Applied Biological Chemistry,2016,59(2):103-106.
[5] Chen L,Zhang X,Hao T,et al.Research progress on antioxidant activity of natural products[J].European Journal of Bio Medical Research,2016,2(1):36-40.
[6] 周统武,蔡娟.大孔吸附树脂纯化茄子叶中的茄尼醇[J].食品与发酵工业,2010(6):175-179.
[7] 唐岚,姜燕清,计燕萍,等.大孔树脂纯化秋茄叶总黄酮及其抗氧化活性研究[J].浙江工业大学学报,2016,44(5):519-523.
[8] Lv D,Cheng X,Tang L,et al.The cardioprotective effect of total flavonoids on myocardial ischemia/reperfusion in rats[J].Biomedicine & Pharmacotherapy,2017,88:277-284.
[9] Liu X Y,Xu L,Wang Y,et al.Protective effects of total flavonoids of Astragalus against adjuvant-induced arthritis in rats by regulating OPG/RANKL/NF-κB pathway[J].International immunopharmacology,2017,44:105-114.
[10] Zhao X,Zhu K,Yi R,et al.Total flavonoid from Ba lotus leaf protected the reserpine-induced gastric ulcer in mice[J].Biomedical Research,2017,28(1):345-352.
[11] Miao M,Yan X,Guo L,et al.Effects of the Rabdosia rubescens total flavonoids on focal cerebral ischemia reperfusion model in rats[J].Saudi Pharmaceutical Journal,2017,25(4):607-614.
[12] He Q S,Zhang L,Fan Z Y,et al.Protective effects of total flavonoids in Caragana against hypoxia/reoxygenation-induced injury in human brain microvascular endothelial cells[J].Biomedicine & Pharmacotherapy,2017,89:316-322.
[13] 周宝利,李兴宝,李腾飞,等.茄子果实中黄酮提取工艺优化及不同茄子品种黄酮含量比较[J].食品科学,2012,33(10):103-106.
[14] 李睿,李万林,郭卫国,等.微波辅助提取茄子根中总黄酮工艺条件研究[J].饲料与畜牧:新饲料,2014 (8):12-15.
[15] 王治业,刘晓风,王鸣刚,等.茄子皮中黄酮的提取工艺研究[J].粮油加工,2014(3):66-68.
[16] 安红钢,林敏,任雪峰,等.茄蒂黄酮提取工艺及抗氧化性研究[J].中成药,2011,33(11):1900-1904.
[17] 莫天录,薛林贵,高慧,等.大孔吸附树脂纯化绿茄叶黄酮的工艺研究[J].天然产物研究与开发,2015,27(3):534-539.
[18] 高海宁,李彩霞,张勇,等.“黑美人”土豆色素体外抗氧化性研究[J].天然产物研究与开发,2012,24(2):224-228,233
[19] 王未,贾清东,毛日文,等.循环超声提取玛咖生物碱动力学及热力学研究[J].食品与生物技术学报,2017,36(5):512-518.
[20] 王春霞,蒲彪,蒋燕,等.藤椒冷榨油饼粕中黄酮类物质的提取及体外抗氧化活性研究[J].食品与机械,2017,33(1):137-143.
[21] 邹小琳,吕兆林,王媛媛,等.竹叶黄酮不同热加工方式稳定性[J].北京林业大学学报,2016,38(11):111-117.
[22] 罗慧玉,闫伟伟,曾春萍,等.HPLC 法同时测定杏花中阿魏酸,芦丁和异槲皮苷[J].实用药物与临床,2017,20(6):701-704.
[22] 马智超,翟春梅,怀雪,等.响应面法优化山荆子果实总黄酮的提取工艺[J].中医药学报,2018,46(5):45-49.
[23] 赵利敏,马绍英,杨海荣,等.响应面分析法优化葡萄籽原花青素的提取工艺[J].甘肃农业大学学报,2012,47(2):120-125.
[24] 王娜,王栋梁,范会平,等.不同提取方法对枣渣黄酮抗氧化活性和得率的影响[J].河南农业大学学报,2015,49(6):843-849.