HPLC法测定阿维拉霉素预混剂中阿维拉霉素的含量
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摘要
目的:采用HPLC法测定10%阿维拉霉素预混剂中阿维拉霉素的含量。方法:测定色谱条件为:流动相:乙腈-0.2%(g/m L)磷酸二氢铵溶液(用磷酸调节p H值至3.0)(51∶49);色谱柱:菲罗门C18(5μm,4.6mm×250mm);检测波长:214nm。结论:阿维拉霉素在0.1~5mg/m L范围内线性关系良好,相关系数R~2为0.9998;阿维拉霉素的回收率为98.7%~101.0%;阿维拉霉素的测定重复性RSD为0.97。结论:该方法具有分析时间短、线性范围宽、定性结果精确和重现性好、样品前处理简便等特点,为阿维拉霉素预混剂的质量控制提供了依据。
Objective:High performance liquid chromatographic method was used for the determination of the contentof Avilamycin in 10% Avilamycin Premixt. Method:Determination of chromatographic conditions:mobile phase:ace-tonitrile:0.2%(g/ml)ammonium dihydrogen phosphate solution(51:49 v/v)with p H value at 3.0(adjusted by phos-phate),column as C18(5μm,4.6mm×250mm),detection wavelength of 214 nm.Conclution:0.1~5mg/m L Avilamy-cin has good linearity,the correlation coefficient R~2 is 0.9998;the recovery rate of avilamycin is 98.7%~101.0%;the detection repeatability RSD of avilamycin is 0.97. Conclution:With features of short testing time,wide linearity,precise assay result,good repeatability,easy pre-treatment of samples and so on,such HPLC assay method providesgood basis for avilamycin premix quality control.
Objective:High performance liquid chromatographic method was used for the determination of the contentof Avilamycin in 10% Avilamycin Premixt. Method:Determination of chromatographic conditions:mobile phase:ace-tonitrile:0.2%(g/ml)ammonium dihydrogen phosphate solution(51:49 v/v)with p H value at 3.0(adjusted by phos-phate),column as C18(5μm,4.6mm×250mm),detection wavelength of 214 nm.Conclution:0.1~5mg/m L Avilamy-cin has good linearity,the correlation coefficient R~2 is 0.9998;the recovery rate of avilamycin is 98.7%~101.0%;the detection repeatability RSD of avilamycin is 0.97. Conclution:With features of short testing time,wide linearity,precise assay result,good repeatability,easy pre-treatment of samples and so on,such HPLC assay method providesgood basis for avilamycin premix quality control.
引文
[1]Irina Treede,Lene Jakobsen,Finn Kirpekar,et al.The avermectins resistance determinants Avi Ra and Avi Rb methylate 23S r RNA at the guanosine 2535 base and ridine 2479 ribose[J].Molecular Microbioligy,2003,49(2):309-318.
[2]陈永辉,刘波,曾兆国,等.阿维拉霉素研究进展[J].饲料工业,2007,28(14):9-11.
[3]任健,童应凯,吴兆亮.卑霉素的合成途径及分子生物学进展[J].天津农学院学报,2008,15(3):47-51.
[4]魏德宝,鲍恩东.饲料中阿维拉霉素含量反相HPLC检测方法的建立[J].南京农业大学学报,2010,33(3):99-103.
[2]陈永辉,刘波,曾兆国,等.阿维拉霉素研究进展[J].饲料工业,2007,28(14):9-11.
[3]任健,童应凯,吴兆亮.卑霉素的合成途径及分子生物学进展[J].天津农学院学报,2008,15(3):47-51.
[4]魏德宝,鲍恩东.饲料中阿维拉霉素含量反相HPLC检测方法的建立[J].南京农业大学学报,2010,33(3):99-103.