田间番茄嫁接口上部茎枯病病原鉴定
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摘要
为明确重庆潼南地区田间番茄嫁接口上部茎枯病病原的具体种类,本试验以嫁接口上部茎秆有坏死症状的番茄幼苗为材料,对其室内保湿培养,通过形态学方法和分子生物学方法进行病原鉴定。确定引起番茄嫁接品种嫁接口上部茎秆坏死症状的病害为灰霉病,病原菌为灰葡萄孢菌。该病原的确定为进一步的药剂筛选奠定了基础。
To identify pathogen of tomato stem blight above grafting interface, tomato seedlings with a necrotic symptom on the upper part of grafting interface were used as the material for moisturizing culture. The results showed that the disease was gray mold, and the pathogen was identified as Botrytis cinerea by morphological and molecular biological methods,which could be contributed to further pesticide screening.
To identify pathogen of tomato stem blight above grafting interface, tomato seedlings with a necrotic symptom on the upper part of grafting interface were used as the material for moisturizing culture. The results showed that the disease was gray mold, and the pathogen was identified as Botrytis cinerea by morphological and molecular biological methods,which could be contributed to further pesticide screening.
引文
[1]刘明.重庆市潼南区农旅融合发展的实践启示[J].重庆行政:公共论坛,2016,17(5):17-18.
[2]Thompson A J,Andrews J,Mulholland B J,et al.Overproduction of abscisic acid in tomato increases transpiration efficiency and root hydraulic conductivity and influences leaf expansion[J].Plant Physiology,2007,143:1905-1917.
[3]Rivard C L,Louws F J.Grafting to manage soilborne disease in heirloom tomato production[J].Hort Science,2008,43:2104-2111.
[4]Mudge K,Janick J,Scofeld S,et al.A history of grafting[J].Horticultural Reviews,2009,35:437-493.
[5]Rivard C L,O’Connell S,Peet M M,et al.Grafting tomato with interspecific rootstock to manage diseases caused by Sclerotium rolfsii and southern root-knot nematode[J].Plant Disease,2010,94(8):1015-1021.
[6]Haroldsen V M,Szczerba M W,Aktas H,et al.Mobility of transgenic nucleic acids and proteins within grafted rootstocks for agricultural improvement[J].Frontiers in Plant Science,2012,3:39-51.
[7]郝芳,周国英,李河.经济林植物病原真菌分类鉴定方法研究进展[J].经济林研究,2009,27(1):112-116.
[8]赵杰.ITS序列分析及其在植物真菌病害分子检测中的应用[J].陕西农业科学,2004(4):35-37.
[9]马明,杨克强,郭启荣.改良CTAB法提取林木树种基因组DNA的研究[J].生物技术,2007,17(3):36-38.
[10]王瑞虎,关鑫,陈秀玲,等.番茄灰霉病菌的鉴定及系统发育树分析[J].北方园艺,2013(6):127-131.
[11]李诚,蒋军喜,赵尚高,等.猕猴桃灰霉病病原菌鉴定及室内药剂筛选[J].植物保护,2014,40(3):48-52.
[12]杨琦凤,尹贤贵,潘光辉,等.重庆春季番茄灰霉病的发生与防治[J].西南园艺,2006,34(3):62-62.
[2]Thompson A J,Andrews J,Mulholland B J,et al.Overproduction of abscisic acid in tomato increases transpiration efficiency and root hydraulic conductivity and influences leaf expansion[J].Plant Physiology,2007,143:1905-1917.
[3]Rivard C L,Louws F J.Grafting to manage soilborne disease in heirloom tomato production[J].Hort Science,2008,43:2104-2111.
[4]Mudge K,Janick J,Scofeld S,et al.A history of grafting[J].Horticultural Reviews,2009,35:437-493.
[5]Rivard C L,O’Connell S,Peet M M,et al.Grafting tomato with interspecific rootstock to manage diseases caused by Sclerotium rolfsii and southern root-knot nematode[J].Plant Disease,2010,94(8):1015-1021.
[6]Haroldsen V M,Szczerba M W,Aktas H,et al.Mobility of transgenic nucleic acids and proteins within grafted rootstocks for agricultural improvement[J].Frontiers in Plant Science,2012,3:39-51.
[7]郝芳,周国英,李河.经济林植物病原真菌分类鉴定方法研究进展[J].经济林研究,2009,27(1):112-116.
[8]赵杰.ITS序列分析及其在植物真菌病害分子检测中的应用[J].陕西农业科学,2004(4):35-37.
[9]马明,杨克强,郭启荣.改良CTAB法提取林木树种基因组DNA的研究[J].生物技术,2007,17(3):36-38.
[10]王瑞虎,关鑫,陈秀玲,等.番茄灰霉病菌的鉴定及系统发育树分析[J].北方园艺,2013(6):127-131.
[11]李诚,蒋军喜,赵尚高,等.猕猴桃灰霉病病原菌鉴定及室内药剂筛选[J].植物保护,2014,40(3):48-52.
[12]杨琦凤,尹贤贵,潘光辉,等.重庆春季番茄灰霉病的发生与防治[J].西南园艺,2006,34(3):62-62.