复方半边莲水溶性药效部位类脂质体制备工艺优化
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摘要
目的:考察影响复方半边莲水溶性药效部位类脂质体制备工艺的各因素,确定复方半边莲水溶性药效部位类脂质体最佳制备工艺条件。方法:以包封率为指标,采用正交试验设计对影响类脂质体质量的司盘-80:胆固醇、载体:药物、水合温度、水合时间进行考察。结果:复方半边莲水溶性药效部位类脂质体最佳制备工艺参数为:司盘-80:胆固醇为1:1,载体:药物为1.05:1,水合介质为PBS(pH=7.6),水合温度为60℃,超声时间10min,水合时间12min。结论:复方半边莲水溶性药效部位类脂质体制备工艺简单,稳定可行,包封率适宜。
Objective:To investigate the factors affecting the preparation process of liposomes from the water-soluble pharmacodynamic fraction of compound lobelia chinensis,and determine its optimum preparation conditions.Methods:Investigated by orthogonal design with encapsulation efficiency as an index.They are respectively the ratio of Sipan 80 to cholesterol,carrier to drug,hydration temperature and hydration time.Results:The optimum preparation parameters of the liposomes of the watersoluble pharmacodynamic fraction of compound lobelia chinensis were as follows:the ratio of Sipan 80 to cholesterol was 1:1,the ratio of carrier to drug was also 1:1,the hydration medium was PBS(pH=7.6),the hydration temperature was 60℃,the ultrasonic time was 10 minutes,and the hydration time was 12 minutes.Conclusion:The preparation process of the liposomes from the water-soluble pharmacodynamics fraction of compound lobelia chinensis is simple,stable and feasible,and the encapsulation efficiency is appropriate.
Objective:To investigate the factors affecting the preparation process of liposomes from the water-soluble pharmacodynamic fraction of compound lobelia chinensis,and determine its optimum preparation conditions.Methods:Investigated by orthogonal design with encapsulation efficiency as an index.They are respectively the ratio of Sipan 80 to cholesterol,carrier to drug,hydration temperature and hydration time.Results:The optimum preparation parameters of the liposomes of the watersoluble pharmacodynamic fraction of compound lobelia chinensis were as follows:the ratio of Sipan 80 to cholesterol was 1:1,the ratio of carrier to drug was also 1:1,the hydration medium was PBS(pH=7.6),the hydration temperature was 60℃,the ultrasonic time was 10 minutes,and the hydration time was 12 minutes.Conclusion:The preparation process of the liposomes from the water-soluble pharmacodynamics fraction of compound lobelia chinensis is simple,stable and feasible,and the encapsulation efficiency is appropriate.
引文
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[2]何建国,许志强,侯云峰.复方半边莲注射液治疗小儿急性支气管炎98例[J].实用中医内科杂志,2004,18(4):366.
[3]吕辉文,许培玲.复方半边莲注射液治疗小儿急性呼吸道感染[J].福建医药杂志,2011,33(5):123-124.
[4]薛宏利.复方半边莲注射液治疗外感高热130例[J].实用中医内科杂志,2002,16(4):235-237.
[5]李娜,于福文.复方半边莲注射液致过敏性休克1例[J].中国医院药学杂志,2005,2(25):191.
[6]严远质.复方半边莲注射液静滴致严重过敏2例[J].药物不良反应杂志,2005,1(7):40.
[7]谭晓黎,慈红丽.复方半边莲注射液致过敏反应4例分析[J].齐鲁药事,2005,7(24):442.
[8]陈琼,李丹,周纪磊.纤维素酶协同超声波法提取复方半边莲注射液的工艺优选[J].中国实验方剂学杂志,2014,7(20):13.
[9]王海燕,叶兆伟,陈琼.纤维素酶法提取制备复方半边莲注射液的工艺条件[J].湖北农业科学,2014,53(20):4946-4948.
[10]国家食品药品监督管理局国家药品标准[S].北京:人民卫生出版社,2002:WS-106091(ZD-0609)-2002.