外源SA对盐胁迫下冬小麦幼苗生长的缓解效应及其机理
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摘要
为明确外源SA缓解冬小麦盐胁迫的适宜浓度。本研究以冬小麦(Triticum aestivum L.)品种‘山农22’为试验材料,水杨酸(SA)作为调控物质,采用室内营养液培养方法,研究5个浓度的SA(50,100,200,300和500μmol/L)对120mmol/L NaCl胁迫下冬小麦幼苗生长、生理特性的影响。结果表明:添加SA能够缓解盐胁迫对小麦造成的损害。200μmol/L SA可以显著提高小麦叶片光合色素含量、根系活力,促进小麦的生长发育;200μmol/L SA可显著提高抗氧化酶活性,增加游离脯氨酸和可溶性蛋白等渗透调节物质含量,缓解膜质过氧化伤害。本研究所采用的SA浓度中,以200μmol/L SA对冬小麦幼苗盐胁迫的缓解效果最佳。
In order to clarify the effects and mechanisms of exogenous SA on the growth of winter wheat seedlings under salt stress and optimize the concentration of exogenous SA alleviating the salt stress.In this study,a hydroponic experiment was carried out on cultivating wheat(Triticum aestivumL.)by using SA as regulatory substance to explore the effects of extraneous SA at 5 concentrations(50,100,200,300 and 500μmol/L)on the growth and physiological properties of wheat under salt stress(120 mmol/L NaCl).The results showed that exogenous SA alleviated salt stress effects in winter wheat seedlings.The alleviating effects were not obvious at 50,100,300 and 500μmol/L SA concentrations,while the concentration of 200μmol/L SA significantly enhanced the chlorophyll content and root activity of wheat seedling.The 200 μmol/L SA treatment significantly improved antioxidant enzymes(SOD,POD,CAT)activities and improved proline content and soluble protein content to alleviate lipid peroxidation damage.In conclusion,the effect of 200μmol/L SA was the best in alleviating salt stress effects of winter wheat seedlings.
In order to clarify the effects and mechanisms of exogenous SA on the growth of winter wheat seedlings under salt stress and optimize the concentration of exogenous SA alleviating the salt stress.In this study,a hydroponic experiment was carried out on cultivating wheat(Triticum aestivumL.)by using SA as regulatory substance to explore the effects of extraneous SA at 5 concentrations(50,100,200,300 and 500μmol/L)on the growth and physiological properties of wheat under salt stress(120 mmol/L NaCl).The results showed that exogenous SA alleviated salt stress effects in winter wheat seedlings.The alleviating effects were not obvious at 50,100,300 and 500μmol/L SA concentrations,while the concentration of 200μmol/L SA significantly enhanced the chlorophyll content and root activity of wheat seedling.The 200 μmol/L SA treatment significantly improved antioxidant enzymes(SOD,POD,CAT)activities and improved proline content and soluble protein content to alleviate lipid peroxidation damage.In conclusion,the effect of 200μmol/L SA was the best in alleviating salt stress effects of winter wheat seedlings.
引文
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[11]张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2005Zhang Z Z,Qu W Q.Plant Physiology Experiment Instruction[M].Beijing:Beijing Higher Education Press,2005(in Chinese)
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[16]Patra H K,Kar M,Mishra D.Catalase activity in leaves and cotyledons during plant development and senescence[J].Biochemie Und Physiologie Der Pflanzen,1978,172(4):385-390
[17]Bradford M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle ofprotein-dye binding[J].Analytical Biochemistry,1976,72(S1/S2):248-254
[18]Khan M N,Siddiqui M H,Mohammad F,Naeem M.Interactive role of nitric oxide and calcium chloride in enhancing tolerance to salt stress[J].Nitric Oxide,2012,27(4):210-218
[19]Horvath E,Szalai G,Janda T.Induction of abiotic stress tolerance by salicylic acid signaling[J].Journal of Plant Growth Regulation,2007,26(3):290-300
[20]Guo Q,Meng L,Mao P C,Jia Y Q,Shi Y J.Role of exogenous salicylic acid in alleviating cadmium-induced toxicity in Kentucky bluegrass[J].Biochemical Systematics and Ecology,2013,50,50:269-276
[21]王弯弯,诸葛玉平,王慧桥,贺明荣,王振林,张吉旺,董元杰.外源NO对盐胁迫下小麦幼苗生长及生理特性的影响[J].土壤学报,2017,54(2):516-524Wang W W,Zhuge Y P,Wang H Q,He M R,Wang Z L,Zhang J W,Dong Y J.Effects of exogenous nitric oxide on growth and physiological characteristics of wheat seedlings under salt stress[J].Acta Pedologica Sinica,2017,54(2):516-524(in Chinese)
[22]王晓立,韩浩章,张颖.水杨酸处理对金钱草光合特性研究[J].江西农业学报,2012,24(10):4-8Wang X L,Han H Z,Zhang Y.Effects of salicylic acid treatment on photosynthetic characteristics of Lysimachia christinae Hance[J].Acta Agriculturae Jiangxi,2012,24(10):4-8(in Chinese)
[23]吴雪霞,朱月林,朱为民,陈建林,刘正鲁.外源一氧化氮对NaCl胁迫下番茄幼苗生理影响[J].中国农业科学,2006,39(3):575-581Wu X X,Zhu Y L,Zhu W M,Chen J L,Liu Z L.Physiological effects of exogenous nitric oxide in tomato seedlings under NaCl stress[J].Scientia Agricultura Sinica,2006,39(3):575-581(in Chinese)
[24]刘庆,董元杰,刘双,张东.外源水杨酸(SA)对NaCl胁迫下棉花幼苗生理生化特性的影响[J].水土保持学报,2014,28(2):165-168Liu Q,Dong Y J,Liu S,Zhang D.Exogenous salicylic acid(SA)on physiological and biochemical characteristics of cotton seedlings under NaCl stress[J].Journal of Soil and Water Conservation,2014,28(2):165-168(in Chinese)
[25]Wang D H,Li X X,Su Z K,Ren H X.The role of salicylic acid in response of two rice cultivars to chilling stress[J].Biologia Plantarum,2009,53(3):545-552
[26]杨瑞红,刘润进,刘成连,王永章,李培环,原永兵.AM真菌和水杨酸对草莓耐盐性的影响[J].中国农业科学,2009,42(5):1590-1594Yang R H,Liu R J,Liu C L,Wang Y Z,Li P H,Yuan Y B.Effects of arbuscular mycorrhizal fungi and salicylic Acid on salt tolerance of strawberry(Fragaria×ananassa Duch)plants[J].Scientia Agricultura Sinica,2009,42(5):1590-1594(in Chinese)
[27]Hoque M A,Banu M N,Nakamura Y,Shimoishi Y,Murata Y.Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells[J].Journal of Plant Physiology,2008,165(8):813-824
[28]周旋,申璐,金媛,毛双双,陈良超,肖斌,肖霄.外源水杨酸对盐胁迫下茶树生长及主要生理特性的影响[J].西北农林科技大学学报:自然科学版,2015,43(7):161-167Zhou X,Sheng L,Jin Y,Mao S S,Chen L C,Xiao B.Effect of exogenous salicylic acid on growth and physiological character of tea plant(Camellia sinensis)under salt stress[J].Journal of Northwest A&F University:Nature Science Edition,2015,43(7):161-167(in Chinese)
[2]周洪华,李卫红.胡杨木质部水分传导对盐胁迫的响应与适应[J].植物生态学报,2015,39(1):81-91Zhou H H,Li W H.Responses and adaptation of xylem hydraulic conductivity to salt stress in Populus euphratica[J].Chinese Journal of Plant Ecology,2015,39(1):81-91(in Chinese)
[3]周万海,冯瑞章,师尚礼,寇江涛.NO对盐胁迫下苜蓿根系生长抑制及氧化损伤的缓解效应[J].生态学报,2015,35(11):3606-3614Zhou W H,Feng R Z,Shi S L,Kou J T.Nitric oxide protection of alfalfa seedling roots against salt-induced inhibition of growth and oxidative damage[J].Acta Ecologica Sinica,2015,35(11):3606-3614(in Chinese)
[4]丁同楼,贾玉辉,鲍敬,王宝山.不同耐盐性小麦根Na+和K+的吸收特性[J].植物生理学报,2013,49(1):34-40Ding T L,Jia Y H,Bao J,Wang B S.The absorption characteristics of Na+and K+in wheat roots with different salt tolerance[J].Plant Physiology Journal,2013,49(1):34-40(in Chinese)
[5]Hayat Q,Hayat S,Irfan M,Ahmad A.Effect of exogenous salicylic acid under changing environment:A review[J].Environmental and Experimental Botany,2010,68(1):14-25
[6]Farheen J,Mansoor S,Abideen Z.Exogenously applied salicylic acid improved growth,photosynthetic pigments and oxidative stability in mungbean seedlings(Vigna radiata)at salt stress[J].Pakistan Journal of Botany,2018,50(3):901-912
[7]Bastam N,Baninasab B,Ghobadi C.Improving salt tolerance by exogenous application of salicylic acid in seedlings of pistachio[J].Plant Growth Regulation,2013,69(3):275-284
[8]Klessig D F,Malamy J.The salicylic acid signal in plants[J].Plant Molecular Biology,1994,26(5):1439-1458
[9]Malamy J,Hennig J,Klessig D F.Temperature-dependent induction of salicylic acid and its conjugates during the resistance response to tobacco mosaic virus infection[J].Plant Cell,1992,4(3):359-366
[10]李天来,李淼,孙周平.钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用[J].应用生态学报,2009,20(3):586-590Li T L,Li M,Sun Z P.Regulation effect of calcium and salicylic acid on defense enzyme activities in tomato leaves under subhigh temperature stress[J].Chinese Journal of Applied Ecology,2009,20(3):586-590(in Chinese)
[11]张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2005Zhang Z Z,Qu W Q.Plant Physiology Experiment Instruction[M].Beijing:Beijing Higher Education Press,2005(in Chinese)
[12]Knudson L L,Tibbitts T W,Edwards G E.Measurement of ozone injury by determination of leaf chlorophyll concentration[J].Plant Physiology,1977,60(4):606-608
[13]Heath R L,Packer L.Photo peroxidation in isolated chloroplasts:I Kinetics and stoichiometry of fatty acid peroxidation[J].Archives Biochemistry Biophysics,1968,125(1):189-198
[14]Stewart R C,Bewley J D.Lipid peroxidation associated with accelerated aging of soybean axes[J].Plant Physiology,1980,65(2):245-248
[15]Nickel R S,Cunningham B A.Improved peroxidase assay method using Ieuco 2,3,6-trichloroindophenol and application to comparative measurements of peroxidase catalysis[J].Analytical Biochemistry,1969,27(2):292-299
[16]Patra H K,Kar M,Mishra D.Catalase activity in leaves and cotyledons during plant development and senescence[J].Biochemie Und Physiologie Der Pflanzen,1978,172(4):385-390
[17]Bradford M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle ofprotein-dye binding[J].Analytical Biochemistry,1976,72(S1/S2):248-254
[18]Khan M N,Siddiqui M H,Mohammad F,Naeem M.Interactive role of nitric oxide and calcium chloride in enhancing tolerance to salt stress[J].Nitric Oxide,2012,27(4):210-218
[19]Horvath E,Szalai G,Janda T.Induction of abiotic stress tolerance by salicylic acid signaling[J].Journal of Plant Growth Regulation,2007,26(3):290-300
[20]Guo Q,Meng L,Mao P C,Jia Y Q,Shi Y J.Role of exogenous salicylic acid in alleviating cadmium-induced toxicity in Kentucky bluegrass[J].Biochemical Systematics and Ecology,2013,50,50:269-276
[21]王弯弯,诸葛玉平,王慧桥,贺明荣,王振林,张吉旺,董元杰.外源NO对盐胁迫下小麦幼苗生长及生理特性的影响[J].土壤学报,2017,54(2):516-524Wang W W,Zhuge Y P,Wang H Q,He M R,Wang Z L,Zhang J W,Dong Y J.Effects of exogenous nitric oxide on growth and physiological characteristics of wheat seedlings under salt stress[J].Acta Pedologica Sinica,2017,54(2):516-524(in Chinese)
[22]王晓立,韩浩章,张颖.水杨酸处理对金钱草光合特性研究[J].江西农业学报,2012,24(10):4-8Wang X L,Han H Z,Zhang Y.Effects of salicylic acid treatment on photosynthetic characteristics of Lysimachia christinae Hance[J].Acta Agriculturae Jiangxi,2012,24(10):4-8(in Chinese)
[23]吴雪霞,朱月林,朱为民,陈建林,刘正鲁.外源一氧化氮对NaCl胁迫下番茄幼苗生理影响[J].中国农业科学,2006,39(3):575-581Wu X X,Zhu Y L,Zhu W M,Chen J L,Liu Z L.Physiological effects of exogenous nitric oxide in tomato seedlings under NaCl stress[J].Scientia Agricultura Sinica,2006,39(3):575-581(in Chinese)
[24]刘庆,董元杰,刘双,张东.外源水杨酸(SA)对NaCl胁迫下棉花幼苗生理生化特性的影响[J].水土保持学报,2014,28(2):165-168Liu Q,Dong Y J,Liu S,Zhang D.Exogenous salicylic acid(SA)on physiological and biochemical characteristics of cotton seedlings under NaCl stress[J].Journal of Soil and Water Conservation,2014,28(2):165-168(in Chinese)
[25]Wang D H,Li X X,Su Z K,Ren H X.The role of salicylic acid in response of two rice cultivars to chilling stress[J].Biologia Plantarum,2009,53(3):545-552
[26]杨瑞红,刘润进,刘成连,王永章,李培环,原永兵.AM真菌和水杨酸对草莓耐盐性的影响[J].中国农业科学,2009,42(5):1590-1594Yang R H,Liu R J,Liu C L,Wang Y Z,Li P H,Yuan Y B.Effects of arbuscular mycorrhizal fungi and salicylic Acid on salt tolerance of strawberry(Fragaria×ananassa Duch)plants[J].Scientia Agricultura Sinica,2009,42(5):1590-1594(in Chinese)
[27]Hoque M A,Banu M N,Nakamura Y,Shimoishi Y,Murata Y.Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells[J].Journal of Plant Physiology,2008,165(8):813-824
[28]周旋,申璐,金媛,毛双双,陈良超,肖斌,肖霄.外源水杨酸对盐胁迫下茶树生长及主要生理特性的影响[J].西北农林科技大学学报:自然科学版,2015,43(7):161-167Zhou X,Sheng L,Jin Y,Mao S S,Chen L C,Xiao B.Effect of exogenous salicylic acid on growth and physiological character of tea plant(Camellia sinensis)under salt stress[J].Journal of Northwest A&F University:Nature Science Edition,2015,43(7):161-167(in Chinese)