国内外产西洋参挥发性成分的HS-SPME/GC-MS比较
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摘要
目的:对不同产地与不同年限的西洋参的挥发性化学成分进行测定分析,为西洋参的进一步开发和利用提供理论依据。方法:采用顶空固相微萃取(HS-SPME)技术结合气相色谱-质谱联用法(GC-MS)对西洋参的挥发性成分进行提取、分析与鉴定,采用色谱峰面积归一化法确定各成分的相对含量。结果:西洋参样品中共鉴定出151种成分,包括烃类99种、醇酚类21种、醛类7种,酮类8种,酯类1种,杂环类及其他成分15种。3年生吉林白山产西洋参根中鉴定出68种成分,质量分数98. 27%; 4年生吉林白山产西洋参根中鉴定出67种成分,质量分数98. 79%; 3年生美国产西洋参根中鉴定出65种成分,质量分数95. 81%; 4年生美国产西洋参根中鉴定出63种成分,质量分数99. 67%。4个西洋参样品的挥发油成分中的特有成分分别为24,23,19和23种,共有成分为16种。结论:不同产地的不同年限的西洋参的挥发性化学成分的含量和组成存在很大差异,该实验结果为未来西洋参药材质量评价研究以及资源的充分合理开发利用提供借鉴。
Objective: To determine the volatile chemical constituents of Panacix Quinquefolii Radix of different origins and different growth years,in order to provide the theoretical basis for the further development and utilization of Panacix Quinquefolii Radix. Method: Headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS) was used to extract, analyze and identify the volatile constituents of Panacix Quinquefolii Radix. The chromatographic peak area normalization method was used to determine the relative amount of ingredients. Result: A total of 151 compounds were identified in Panacix Quinquefolii Radix samples,including 99 hydrocarbons,21 alcohol phenols,7 aldehydes,8 ketones,1 ester,15 heterocyclics and other compounds. Totally 68 kinds of compounds were identified in the roots of three-year-old Jilin Baishan,and the mass fraction accounted for 98. 27%,67 compounds were identified in the roots of fouryear-old Jilin Baishan,and the mass fraction accounted for 98. 79%,65 compounds were identified in 3-year-old Panacix Quinquefolii Radix root,and the mass fraction accounted for 95. 81%,and 63 compounds were identified in 4-year-old Panacix Quinquefolii Radix root, and the mass fraction accounted for 99. 67%. The specific components of the volatile oil components of the four Panacix Quinquefolii Radix samples were 24,23,19,and23,respectively,and the total composition was 16 species. Conclusion: The content and composition of volatile chemical constituents of Panacix Quinquefolii Radix of different origins are very different. This experiment provides a reference for the future quality evaluation of Panacix Quinquefolii Radix medicinal materials, rational development and utilization of resources.
Objective: To determine the volatile chemical constituents of Panacix Quinquefolii Radix of different origins and different growth years,in order to provide the theoretical basis for the further development and utilization of Panacix Quinquefolii Radix. Method: Headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS) was used to extract, analyze and identify the volatile constituents of Panacix Quinquefolii Radix. The chromatographic peak area normalization method was used to determine the relative amount of ingredients. Result: A total of 151 compounds were identified in Panacix Quinquefolii Radix samples,including 99 hydrocarbons,21 alcohol phenols,7 aldehydes,8 ketones,1 ester,15 heterocyclics and other compounds. Totally 68 kinds of compounds were identified in the roots of three-year-old Jilin Baishan,and the mass fraction accounted for 98. 27%,67 compounds were identified in the roots of fouryear-old Jilin Baishan,and the mass fraction accounted for 98. 79%,65 compounds were identified in 3-year-old Panacix Quinquefolii Radix root,and the mass fraction accounted for 95. 81%,and 63 compounds were identified in 4-year-old Panacix Quinquefolii Radix root, and the mass fraction accounted for 99. 67%. The specific components of the volatile oil components of the four Panacix Quinquefolii Radix samples were 24,23,19,and23,respectively,and the total composition was 16 species. Conclusion: The content and composition of volatile chemical constituents of Panacix Quinquefolii Radix of different origins are very different. This experiment provides a reference for the future quality evaluation of Panacix Quinquefolii Radix medicinal materials, rational development and utilization of resources.
引文
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[6]王倩.糖脂平方治疗2型糖尿病胰岛素抵抗(气阴两虚兼痰瘀证)的临床研究[D].兰州:甘肃中医药大学,2016.
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[11]唐艳,闫述模,汪静静,等.基于UPLC及多成分分析的西洋参质量评价[J].中国中药杂志,2016,41(9):1678-1684.
[12]张丹,郑开颜,吴兰芳,等.西洋参药材的质量特征与商品规格的相关性分析[J].中国中药杂志,2016,41(18):3329-3335.
[13]郑友兰,张崇禧,李向高,等.国产西洋参与进口西洋参的比较研究一西洋参中挥发油成分的分析[J].药学学报,1989,24(2):118-121.
[14]常宇桐,罗云敬,钱承敬,等.顶空固相微萃取-气相色谱质谱法测定馥郁香型白酒中的挥发性香气成分[J].食品安全质量检测学报,2018,9(17):4618-4627.
[15]吴稚冰,马胜林.β-榄香烯抗肿瘤作用的研究进展[J].中华中医药学刊,2011,29(10):2255-2257.
[16]谢小洋,冯永忠,王得祥. 5种园林树木挥发性成分分析[J].西北农林科技大学学报:自然科学版,2016,44(7):146.
[2]何晓燕,王兵,朱卫兵.西洋参的化学成分与药理作用[J].益寿宝典,2017,5(32):56-57.
[3] ZHUO D R,QUE H T,ZHANG N,et al. In vitro dissolution rate of ginsenoside Rb1in superfine powder of wild Panacis Quinquefolii Radix[J]. Drug Eval Res,2013,36:374-376.
[4] MAO J,Juan M M,Tom C,et al. American ginseng acutely regulates contractile function of rat heart[J].Fronti Pharmacol,2014,5:43.
[5] Assinewe A V,Baum R B,Gagnon D,et al.Phytochemistry of wild populations of Panax quinquefolius L.(North American ginseng)[J]. J Agric Food Chem,2003,51(16):4549-4553.
[6]王倩.糖脂平方治疗2型糖尿病胰岛素抵抗(气阴两虚兼痰瘀证)的临床研究[D].兰州:甘肃中医药大学,2016.
[7] Singh R K,Lui E,Wright D,et al. Alcohol extract of North American ginseng(Panax quinquefolius)reduces fatty liver,dyslipidemia,and other complications of metabolic syndrome in a mouse model[J]. Can J Physiol Pharmacol,2017,95:1046-1057.
[8] Mucalo I,Jovanovski E,Raheli D,et al. Effect of American ginseng(Panax quinquefolius L.)on arterial stiffness in subjects with type-2 diabetes and concomitant hypertension[J]. J Ethnopharmacol,2013,150:148-153.
[9] Dougherty U,Mustafi R,WANG Y,et al. American ginseng suppresses Western dietpromoted tumorigenesis in model of inflammation-associated colon cancer:role of EGFR[J]. BMC Complementary and Alternative Medicine,2011,11:1-11.
[10] Sandra C M,Danielle D,Jacqueline J S. Extract of North American ginseng(Panax Quinquefolius),administered to leukemic,juvenile mice extends their life Span[J]. J Complement Inteqr Med,2011,8:1553-3840.
[11]唐艳,闫述模,汪静静,等.基于UPLC及多成分分析的西洋参质量评价[J].中国中药杂志,2016,41(9):1678-1684.
[12]张丹,郑开颜,吴兰芳,等.西洋参药材的质量特征与商品规格的相关性分析[J].中国中药杂志,2016,41(18):3329-3335.
[13]郑友兰,张崇禧,李向高,等.国产西洋参与进口西洋参的比较研究一西洋参中挥发油成分的分析[J].药学学报,1989,24(2):118-121.
[14]常宇桐,罗云敬,钱承敬,等.顶空固相微萃取-气相色谱质谱法测定馥郁香型白酒中的挥发性香气成分[J].食品安全质量检测学报,2018,9(17):4618-4627.
[15]吴稚冰,马胜林.β-榄香烯抗肿瘤作用的研究进展[J].中华中医药学刊,2011,29(10):2255-2257.
[16]谢小洋,冯永忠,王得祥. 5种园林树木挥发性成分分析[J].西北农林科技大学学报:自然科学版,2016,44(7):146.